Method for inactivating viruses in plasma

A technology of virus inactivation and blood plasma, applied in disinfection, irradiation, chemistry, etc., to achieve the effect of overcoming thermal effects and high safety

Active Publication Date: 2020-02-21
蓝铂医疗科技发展(苏州)有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The above two most commonly used plasma virus inactivation methods (S / D inactivation method, methylene blue photochemical treatment method) only inactivate enveloped viruses, but have no inactivation effect on non-enveloped viruses ; Even after being treated with these two methods, there is still a certain virus safety risk in plasma

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  • Method for inactivating viruses in plasma
  • Method for inactivating viruses in plasma
  • Method for inactivating viruses in plasma

Examples

Experimental program
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Effect test

Embodiment 1

[0032] Embodiment 1 main reagent, raw material and instrument

[0033] The plasma used in the experiment was fresh frozen dog plasma purchased from Beijing Tianqin Yihe Biotechnology Co., Ltd.;

[0034] Pseudorabies virus and encephalomyocarditis virus were purchased from China Veterinary Microbiological Culture Collection and Management Center;

[0035] PK-15 and BHK-21 cells were purchased from China Veterinary Microbiology Culture Collection and Management Center;

[0036] Medicinal rutin is produced by Sichuan Xieli Pharmaceutical Co., Ltd.;

[0037] Rutin is produced by SIGMA / MERCK;

[0038] The plasma virus inactivator was developed by Zhongke Shisheng (Beijing) Pharmaceutical Technology Co., Ltd.;

[0039] The rest of the reagents and instruments are conventional domestic models.

Embodiment 2

[0040] The virus inactivation and detection of embodiment 2 blood plasma

[0041] 1 Preparation of poisoned plasma: Thaw frozen plasma, take 100ML and put it in a plasma bag, add medicinal rutin or rutin in proportion, and then mix the indicator virus liquid and plasma at a ratio of 1:9; with capsule indication Pseudorabies virus (PRV) was selected as the virus, and encephalomyocarditis virus (EMCV) was selected as the non-enveloped indicator virus.

[0042] 2 inactivation process: use 25 # The silicone tube connects the plasma bag with the sample inlet of the plasma virus inactivation instrument, and the sample outlet is also connected with 25 # Connect the silicone tube to the plasma collection bag; turn on the UVC light source (UVC intensity: 500-5000μw / cm 2 ) After the light intensity is stable, turn on the power of the peristaltic pump, pump the poisoned plasma into the spiral quartz glass inactivation tube, so that the plasma is completely treated with radiation inacti...

Embodiment 3

[0052] Each group of sample processing of embodiment 3

[0053] Due to space reasons, all combinations of proportional concentration orthogonal experiments are not shown, and only some representative results are shown to show the basic trend. Plasma was exposed to the virus in proportion, each sample was added with different amounts of medicinal rutin or rutin, and different irradiation methods were used to verify the inactivation effect on pseudorabies virus and encephalomyocarditis virus.

[0054] 1 The inactivation treatment method of adding medicinal rutin as a protective agent and enhancer is as follows:

[0055] (Sample 1) Add rutin injection 0.3g / L to the poisoned plasma, mix well and put it into the inactivation instrument. 2 , irradiation treatment for 1200s.

[0056] (Sample 2) Add rutin injection 0.3g / L to the poisoned plasma, put it into the inactivator after mixing, and put it into the inactivation instrument under the light intensity of 5000μw / cm 2 , irradiati...

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Abstract

The invention provides a method for inactivating viruses in plasma. The method comprises the step of adding medicinal rutin of 0.3-0.8 g/L or rutin sophorin of 0.5-1.5 mM to the plasma, wherein the rutin or rutin sophorin is adopted as an enhancer of to-be-inactivated viruses and a protective agent for plasma protein. Envelope viruses and non-enveloped viruses in the plasma can be inactivated effectively through UVC irradiation of certain intensity, and the problem that non-enveloped viruses cannot be inactivated through a methylene blue photochemical method is solved. Through the method, lossof protein during plasma virus inactivation can be reduced, especially loss of clotting factors is reduced, and the activity of the clotting factors in the plasma can be protected effectively.

Description

[0001] a technical field [0002] The invention belongs to the field of blood products and the field of virus inactivation. Specifically, the invention provides a plasma virus inactivation method, which comprises adding 0.3g / L-0.8g / L of medicinal rutin or 0.5mM rutin to plasma -1.5mM, as a protein protectant and synergist; this method can effectively reduce the loss of proteins, especially blood coagulation factors, in the photochemical inactivation of plasma viruses, and effectively kill enveloped viruses and non-enveloped viruses. [0003] Two background technology [0004] Plasma is a necessity in many links such as surgery and rescue in medical treatment, and it is also a raw material for preparing various blood products. At present, plasma is mainly obtained through human donation, and virus contamination is generally controlled through screening methods during donation. Due to the certain window period and false negative rate of screening, in order to further increase the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L2/10A61L2/16A61L101/44
CPCA61L2/0047A61L2/0082A61L2202/22
Inventor 吕茂民武岳朱凤宣梁梦雅孙珍珠董秋瑛屈洒洒方迟张梦君李庆保
Owner 蓝铂医疗科技发展(苏州)有限公司
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