Application of exosome derived from carcass in skin regulation product

A technology of skin regulation and exosomes, applied in skin care preparations, skin diseases, applications, etc.

Inactive Publication Date: 2020-02-28
PHARCHOICE THERAPEUTICS INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Therefore, there is no prior art disclosing or suggesting the use of ex

Method used

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  • Application of exosome derived from carcass in skin regulation product
  • Application of exosome derived from carcass in skin regulation product
  • Application of exosome derived from carcass in skin regulation product

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0084] Embodiment 1, the preparation of fetal goat serum exosome

[0085] Filter the fetal sheep serum of 4-month-old alpine black sheep with a 0.45 μm filter membrane, centrifuge at 1000g for 10 minutes at 4°C, and collect the supernatant; collect the supernatant by centrifuging at 2000g for 20 minutes at 4°C; Centrifuge at 10,000g for 30min at 4°C to collect the supernatant; centrifuge the collected supernatant at 110,000g for 90min, discard the supernatant, and resuspend the pellet with phosphate buffer; centrifuge again at 110,000g for 90min, discard the supernatant, and resuspend with a small amount of phosphate buffer The precipitate was filtered through a 0.45 μm filter membrane to obtain exosomes. The total protein content of exosomes was detected by Bradford method (Bio-Rad Protein Assay Reagent). Exosomes were lyophilized and stored at -80°C. Adult sheep serum was used to extract exosomes by the same method as control exosomes.

Embodiment 2

[0086] Example 2, Preparation of Fetal Camel Serum Exosomes

[0087] Filter the 11-month-old Australian alpaca serum with a 0.45 μm filter membrane, centrifuge at 4°C, 1000g for 10min, and collect the supernatant; collect the supernatant by centrifuging at 4°C, 2000g for 20min, and collect the supernatant; , centrifuge at 10000g for 30min, collect the supernatant; centrifuge the collected supernatant at 110000g for 90min, discard the supernatant, and resuspend the pellet with phosphate buffer; Filter with a 0.45 μm filter membrane to obtain exosomes. The total protein content of exosomes was detected by Bradford method (Bio-Rad Protein Assay Reagent). Exosomes were lyophilized and stored at -80°C. The same method was used to extract exosomes from adult alpaca serum, which was used as control exosomes.

Embodiment 3

[0088] Embodiment 3, the preparation of fetal bovine serum exosome

[0089] The 8-month-old Australian fetal bovine serum was filtered with a 0.22 μm sterile membrane, and the supernatant was collected by centrifugation at 1000 g at 4 ° C for 10 min; the collected supernatant was centrifuged at 4 ° C and 2000 g for 20 min, and the supernatant was collected; Centrifuge at 10000g for 30min at ℃, collect the supernatant; centrifuge the collected supernatant at 110000g for 90min, discard the supernatant, and resuspend the pellet with phosphate buffer; centrifuge again at 110000g for 90min, discard the supernatant, and resuspend the pellet with a small amount of phosphate buffer , and filtered through a 0.45 μm filter membrane to obtain exosomes. The total protein content of exosomes was detected by Bradford method (Bio-Rad Protein Assay Reagent). Exosomes were lyophilized and stored at -80°C.

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Abstract

The invention provides an application of an exosome derived from a carcass in a skin regulation product. Experiments prove that the exosome realizes an anti-aging function by promoting cell collagen expression, increasing cell myofibrillar protein synthesis, maintaining stem cell dryness and stimulating stem cell proliferation; the exosome realizes a pharmaceutical function of anti-acne by inhibiting products of gene expression of inflammatory mediators or matrix protease in keratinocytes and inhibiting the synthesis and release of VEGF in keratinocytes; the exosome realizes a dermatological application of enhancing hair follicles and resisting alopecia by promoting ATP synthesis in hair follicle papilla fibroblasts or products for enhancing mitochondrial metabolic activity, increasing cell viability of micro hair follicles or reducing the damage of micro hair follicle membranes; and the exosome provides a new approach for skin care such as anti-aging skin care, acne resistance, alopecia prevention, dust impact prevention and the like by removing and eliminating toxins, increasing the expression of skin antimicrobial peptide hBD3, inhibiting the production of active oxygen and preventing skin problems caused by Asian dust.

Description

technical field [0001] The invention belongs to the technical field of biological skin regulation products, and specifically relates to the use of exosomes derived from carcass in skin regulation products and the skin regulation products containing the exosomes. Background technique [0002] In the field of cosmetics, the concept and biomedical effects of "sheep placenta" are controversial. The earlier record is that in 1931, Dr. Paul Niehans of Switzerland injected fresh parathyroid cells derived from lamb fetuses for the first time. This is the original origin of fetal sheep cell activation therapy. The discovery of Niehans has a milestone significance for the development of human medicine in the field of anti-aging. Therefore, Paul Niehans is known as the "father of living cells" and "the founder of fetal sheep cell activation therapy". .Paul Niehans tribute, conferring Paul Niehans as a member of the Vatican Academy of Sciences. [0003] The descriptive literature does...

Claims

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Application Information

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IPC IPC(8): A61K8/98A61Q19/08A61P17/10A61P17/14A23L33/10A23C19/09A23C15/12A23C9/152A23G9/36A23C9/13A23D7/005A23D9/007A21D13/06A21D2/34A23G3/36A23G4/12A23L2/52
CPCA21D2/34A21D13/06A23C9/13A23C9/152A23C15/12A23C19/09A23D7/005A23D9/007A23G3/364A23G4/12A23G9/36A23L2/52A23V2002/00A61K8/982A61Q19/08A23L33/10A61P17/10A61P17/14A23V2200/302A23V2200/318A23V2250/204
Inventor 傅文燕胡适
Owner PHARCHOICE THERAPEUTICS INC
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