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Method for improving saponin yield by low-acid pretreatment of dioscorea composita

A technology of diosminum chrysanthemum and acid pretreatment, applied in the direction of fermentation, etc., can solve the problems of pollution, low hydrolysis yield, etc., achieve the effect of improving hydrolysis yield, avoiding long pretreatment period, and good promotion and application value

Inactive Publication Date: 2020-02-28
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to provide a method for improving saponin yield by low-acid pretreatment of Dioscorea chrysanthemi in order to overcome the problems of low hydrolysis yield and serious pollution faced by the current diosgenin hydrolysis production in the prior art

Method used

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  • Method for improving saponin yield by low-acid pretreatment of dioscorea composita
  • Method for improving saponin yield by low-acid pretreatment of dioscorea composita
  • Method for improving saponin yield by low-acid pretreatment of dioscorea composita

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] The optimization of the acid hydrolysis condition of embodiment 1 diosgenin

[0051] One, the acid hydrolysis method of diosgenin

[0052] Take Dioscorea chrysanthemum freeze-dried powder and put it into a 50mL reaction kettle, add sulfuric acid solution according to the ratio of solid to liquid of 1:6, heat at 120°C, after the hydrolysis reaction is completed, cool to room temperature, then neutralize with NaOH, filter, and dry to obtain hydrolysis thing. The hydrolyzate was extracted with a Soxhlet extractor, petroleum ether (boiling range 60-90°C), and refluxed in a water bath at 90°C. After the solvent was recovered, the extract was dissolved in methanol to constant volume, and the content of diosgenin was determined by HPLC.

[0053] 2. Effect of sulfuric acid concentration on the acid hydrolysis of diosgenin

[0054] Select different concentrations of sulfuric acid (0.6mol / L, 0.8mol / L, 1.0mol / L, 1.2mol / L and 1.6mol / L) respectively, according to the solid-liquid ...

Embodiment 2

[0057] Example 2 Production of diosgenin by acid hydrolysis of Dioscorea chrysanthemum leaf after low-acid pretreatment

[0058] Select sulfuric acid with a concentration of 0.1mol / L, the temperature is 105°C, and the ratio of solid to liquid is 1:6. Pre-hydrolyze. g (water / raw material), centrifuge solid-liquid separation again, measure residual solids content, then, carry out the acid hydrolysis of diosgenin with the solid-to-liquid ratio of 1:6, pre-acidolysis is to the acid that diosgenin hydrolysis consumes and The COD in the waste acid water was analyzed,

[0059] Specifically, select sulfuric acid with a concentration of 0.1mol / L, the temperature is 105°C, the ratio of solid to liquid is 1:6, pre-hydrolyze for 6 hours, and directly centrifuge for solid-liquid separation, take the solid, and then make up to 6ml / g with deionized water (water / raw material), re-centrifuge solid-liquid separation, measure remaining solid content, then, carry out the acid hydrolysis of diosg...

Embodiment 3

[0060] The impact of embodiment 3 prehydrolysis on saponin

[0061] 1. Experimental method

[0062] Sulfuric acid with a concentration of 0.1mol / L is selected, the temperature is 105°C, and the ratio of solid to liquid is 1:6 for prehydrolysis treatment. Detect the content of steroidal saponin content after pretreatment.

[0063] 2. Experimental results

[0064] The change result of steroidal saponin content (1.0g Dioscorea chrysanthemi hydrolyzate system) after pretreatment is as follows image 3 .

[0065] The reduction of furostane saponin content and the increase of spirosterane saponin are the key to the improvement of diosgenin hydrolysis yield. In the process of low-acid hydrolysis, furostane saponin ( image 3 b and image 3 d) The content in the system is all reduced, wherein the content of 25-O-protodioscin is basically zero at 4 hours, and the furostane saponin asperoside with a lower content is also completely transformed after 3 hours; Alkanosides ( image ...

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Abstract

The invention discloses a method for improving saponin yield by low-acid pretreatment of dioscorea composita. The method comprises the steps of pretreating dioscorea composita with low-concentration sulfuric acid, carrying out solid-liquid separation, and hydrolyzing diosgenin in a solid phase part. The problems of long pretreatment period of natural fermentation and potential risk of generating impurities are avoided; meanwhile, in a process of direct hydrolysis of the diosgenin, because starch and lignocellulose are hydrolyzed and further dehydrated, the content of organic matters in the hydrolyzed waste acid water is sharply increased, and serious environmental pollution problems are caused; and in addition, the hydrolysis yield of the diosgenin is obviously improved. The method has obvious advantages and good popularization and application values.

Description

technical field [0001] The invention relates to the technical field of diosgenin extraction, and more specifically, relates to a method for improving saponin yield through low-acid pretreatment of dioscin chrysanthemum leaf. Background technique [0002] Dioscorea chrysanthemum is a plant of the genus Dioscorea in the Dioscoreaceae family. Originally produced in Mexico, it was successfully introduced to China's Yunnan Province in the 1970s for trial planting, and is currently widely planted in my country. Chrysanthemum leaf diosgenin content is relatively high, and its saponin content is about twice that of diosminum shield leaf. It is an important raw material for extracting saponin and synthesizing other corticosteroids. [0003] The steroidal structure of diosgenin is simple and easy to chemically modify. It is the main raw material for the synthesis of steroid hormone drugs, the second largest drug after antibiotics. It is called medical gold and has a high market value...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P33/20
CPCC12P33/20
Inventor 谢君叶广英毕桂灿
Owner SOUTH CHINA AGRI UNIV
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