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A method for improving the efficiency of cellulose enzymatic hydrolysis of rice straw by using lactic acid and guanidine hydrochloride

A technology of rice straw and cellulase, applied in the direction of fermentation, etc., can solve the problems of unfriendly environment and high cost, and achieve the effect of overcoming unfriendly environment, low cost, reducing equipment requirements and operation difficulty

Inactive Publication Date: 2020-02-21
GUANGDONG UNIV OF TECH
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Problems solved by technology

Using lactic acid / guanidine hydrochloride as the lignocellulose pretreatment solvent overcomes the disadvantages of the traditional ionic liquid pretreatment process, such as unfriendly environment and high cost

Method used

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  • A method for improving the efficiency of cellulose enzymatic hydrolysis of rice straw by using lactic acid and guanidine hydrochloride
  • A method for improving the efficiency of cellulose enzymatic hydrolysis of rice straw by using lactic acid and guanidine hydrochloride
  • A method for improving the efficiency of cellulose enzymatic hydrolysis of rice straw by using lactic acid and guanidine hydrochloride

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Embodiment 1

[0021] Lactic acid / guanidine hydrochloride (molar ratio 1:1) pretreatment improves rice straw enzymatic hydrolysis efficiency

[0022] a) Pretreatment: Accurately weigh 300mg of rice straw powder (the rice straw is dried and crushed into rice straw powder with an average particle size of 150-350μm) and 6g of lactic acid / guanidine hydrochloride deep eutectic solvent with a molar ratio of 1:1 , placed together in a 25mL Erlenmeyer flask, refluxed and stirred at 120°C for 6 hours; then cooled to room temperature, diluted with 6g of warm water, filtered, and then washed with 18g of deionized water for 4 times, and the filter residue was pretreated after drying After the rice straw.

[0023] b) Enzymolysis: Accurately weigh 20 mg of the above-mentioned pretreated rice straw, place it in a 50 mL Erlenmeyer flask, add 7 mL of citrate buffer solution (50 mmol / L, pH 4.8) and 160 U of the commercial product derived from Trichoderma reesei Cellulase was sealed and placed in a constant tem...

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Abstract

The invention belongs to the fields of lignocellulose utilization and energy and chemical application, and discloses a green method for increasing a cellulose enzymolysis efficiency in rice straws by utilizing lactic acid / guanidine hydrochloride to extract hemicellulose. The green method includes: taking the lactic acid / the guanidine hydrochloride as a solvent to preprocessing the rice straws, filtering and separating to obtain residues, and drying to obtain preprocessed rice straws; taking the rice straw residues as a base material, and subjecting the base material to enzymolysis by cellulose so as to obtain a liquid sugar which mainly comprises glucose. The green method has the advantages that the enzymolysis efficiency of the rice straws can be increased effectively, a yield rate of fermentable reducing sugar is increased, and the defects of high cost and environmental unfriendliness of an ionic liquid preprocessing process are overcome.

Description

technical field [0001] The invention belongs to the field of lignocellulose utilization and energy chemical industry application, and specifically relates to a green method for extracting hemicellulose by using lactic acid / guanidine hydrochloride to improve the enzymatic hydrolysis efficiency of cellulose in rice straw. Background technique [0002] With the outbreak of the global energy crisis and the intensification of environmental pollution, the replacement of fossil energy and fossil products with clean and renewable biomass fuels and biomass-based platform compounds has attracted extensive attention. Among them, the production of biofuels and related platform compounds from a wide range of sources, cheap and renewable lignocellulosic biomass such as agricultural wastes is the most promising. The main components of lignocellulosic biomass are cellulose (40-50 wt%), hemicellulose (20-40 wt%) and lignin (15-30 wt%). Due to differences in species, sources, and parts, the ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/14C12P19/02
CPCC12P19/02C12P19/14C12P2201/00C12P2203/00
Inventor 侯雪丹冯国坚林凯鹏李奥林
Owner GUANGDONG UNIV OF TECH
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