Exosome transdermal delivery preparation and use thereof
A technology of transdermal exosomes and exosomes, which is applied in the field of transdermal exosome delivery preparations to achieve the effect of improving skin absorption and expanding the scope of application
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Embodiment 1
[0024] Example 1 The promotion effect of bee spongy bone on the skin absorption of model exosomes
[0025] In vitro transdermal test: Take out the frozen skin, use a puncher to take a round skin (28 mm in diameter) and thaw it at room temperature for half an hour, and wash the skin surface with distilled water. In this experiment, the integrity of the skin is evaluated by the conductivity of the skin. If the skin resistance is greater than 30KΩ-cm2, the skin surface is considered complete and suitable for subsequent penetration experiments. The skin surface was placed on the Franz diffusion cell, and the receiving cell was filled with PBS solution. Exosomes are delivered in the following four ways:
[0026] Exosome group: add 150 μL of fluorescently labeled exosome solution to the administration pool;
[0027] Exosome (massage) group: use an electric massage stick to massage the skin for 2 minutes, and add 150 μL of fluorescently labeled exosome solution;
[0028] Exosome (...
Embodiment 2
[0033] Using protein-labeled fluorescent exosomes from human umbilical cord mesenchymal stem cells as model exosomes, the skin absorption of exosomes in living mice was compared with or without the use of bee sponge bone needles to promote penetration, as shown in image 3 shown.
[0034] method:
[0035] Under isoflurane anesthesia, an electric shaver was used to remove the hair of six-week-old SPF Kunming mice at the administration site, and the Franz administration pool was fixed at the administration site with animal tissue glue. Exosome (bone needle) group: add 10 mg / 100 μL bee sponge bone needle PBS suspension into the administration pool, and use an electric massage stick to massage for 2 minutes. The residual bone spicules on the skin surface were washed with PBS, and 150 μL of fluorescently labeled exosome solution was added. Exosome group: Add 150 μL of fluorescently labeled exosome solution directly into the dosing pool. After the mice were transdermally anesthet...
Embodiment 3
[0037] Using human umbilical cord mesenchymal stem cell exosomes as the activity test exosomes, through the ultraviolet-induced mouse skin photoaging model, to evaluate the exocytosis of umbilical cord mesenchymal stem cells with or without the use of bee sponge bone needles to promote infiltration The anti-aging effect of the body, the specific evaluation criteria include skin wrinkle changes ( Figure 4 ), pathological changes of skin tissue (including epidermal thickness) ( Figure 4 , 5), the expression changes of related physiological factors ( Figure 6 ).
[0038] method:
[0039] Establishment of mouse skin photoaging model and drug administration in groups: six-week-old SPF Kunming mice were put into a self-made airtight irradiation box and fixed. The light source is about 43cm away from the skin. The lamp tube needs to be preheated for 15-30 minutes, the irradiated animals are exposed to ultraviolet radiation for irradiation, and the ultraviolet meter measures th...
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