Burkholderia arboria MP5 strain with antibacterial effect and application thereof
A Burkholderia and bacteriostatic technology, applied in the field of Burkholderia forest MP5 strains, can solve problems such as affecting the sustainable and healthy development of agriculture, endangering the health of consumers, and excessive pesticide residues. , to achieve the effect of good market application prospects
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[0024] 1. Isolation and screening of strains
[0025] On June 20, 2017, 20 tomato root samples were collected in Laoshan, Qingdao. The samples were transferred to a sterilized mortar for grinding, diluted with sterile water to 10ml, and then diluted to 10ml with sterile water. -4 -10 -5 times, take 100ul respectively and apply LB plates (tryptone 10 g / L, yeast extract 5 g / L, sodium chloride 5 g / L, pH 7.0), and culture at 28°C.
[0026] Pick a single colony, and inoculate a single bacterial colony in YEPD (yeast extract 3g / l, peptone 10g / l, glucose 20g / l) liquid medium for shaking at 25°C-28°C for 96h, then 4°C at 9000rpm After centrifugation, the supernatant was filtered through a filter with a diameter of 0.22um for later use. According to different proportions, the filtrate was added to the YPD medium, and the conidia of Botrytis cinerea and Fusarium oxysporum were inoculated for static culture, and the spores were inoculated into YEPD as a control. When the germination ra...
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