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In-capillary microextraction-nanoliter electrospray ion source system and application

An electrospray ion source and capillary technology, applied in the field of chemistry, to achieve ideal reproducibility and high sensitivity

Inactive Publication Date: 2020-03-17
GUANGDONG INST OF ANALYSIS CHINA NAT ANALYTICAL CENT GUANGZHOU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although atmospheric open mass spectrometry provides a new technical means for rapid and direct characterization of biological lipids, establishing a systematic and complete lipidomics approach using atmospheric open mass spectrometry is still a daunting task

Method used

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  • In-capillary microextraction-nanoliter electrospray ion source system and application
  • In-capillary microextraction-nanoliter electrospray ion source system and application

Examples

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Comparison scheme
Effect test

Embodiment 1

[0025] Example 1: A micro-extraction-nanoliter electrospray ion source system in a capillary

[0026] A micro-extraction-nano-electrospray ion source system in a capillary, the system includes a micro-scale sampling probe, a nano-electrospray needle and a high-voltage power supply; the micro-scale sampling probe directly performs micro-scale sampling on biological tissue samples and collects The trace samples obtained are directly brought into the nano-electrospray needle, and the solid-liquid microextraction is carried out in the capillary of the nano-electrospray needle, and then the field-induced nano-electrospray mass spectrometry is directly carried out under the condition of open atmospheric pressure. The micro-scale sampling probe is a surface-treated tungsten needle with a tip diameter in the range of 1 μm.

Embodiment 2

[0027] Example 2: A micro-extraction-nanoliter electrospray ion source system in a capillary

[0028] The system includes a micro-scale sampling probe, a nano-electrospray needle, a UV lamp with a wavelength of 254nm that can be used for Paternò-Büchi photochemical reactions, and a high-voltage power supply; the micro-scale sampling probe directly performs micro-scale sampling on biological tissue samples and collects A small amount of sample is directly brought into the nano-electrospray needle, and the solid-liquid microextraction is carried out in the capillary of the nano-electrospray needle. The PB online photochemical reaction is then directly carried out under the condition of normal pressure and open field-induced nano-electrospray. Mass Spectrometry.

Embodiment 3

[0029] Embodiment 3: the method for the direct analysis of biological sample

[0030] Using a mass spectrometry device, the mass spectrometry device includes the micro-extraction-nanoliter electrospray ion source system in the capillary described in Example 2, the nano-liter electrospray ion source system that is used to fix the micro-extraction-nanoliter electrospray ion source system in the capillary. The tip of the electrospray needle is aimed at the entrance of the mass spectrometer and is 5-15mm away from the entrance of the mass spectrometer, preferably a support of 10mm; it includes the following steps: using a micro-scale sampling probe for direct sampling of biological tissue samples, and provoking it through the tip of the probe And adhere a certain micro amount of biological tissue sample (volume not more than 0.5mm×0.5mm×0.5mm) to the tip of the probe. Then, the probe and the biological tissue sample adhered to the tip of the probe are inserted together into a tube c...

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Abstract

The invention discloses an in-capillary microextraction-nanoliter electrospray ion source system and an application. The in-capillary microextraction-nanoliter electrospray ion source system comprisesa micro-scale sampling probe, a nanoliter electrospray needle and a high-voltage power supply. The in-capillary microextraction-nanoliter electrospray ion source system achieves the online combination of three technologies of solid-liquid micro-extraction, Paterno-Buchi online photochemical reaction and normal-pressure open type field-induced nanoliter electrospray ionization mass spectrometry, can be used as a novel nanoliter electrospray ionization source for mass spectrometry, has extremely high sensitivity, and can be used for in-situ, direct and micro-scale lipid analysis and omics research of complex biological samples.

Description

Technical field: [0001] The invention relates to the field of chemistry, in particular to a capillary micro-extraction-nano-electrospray ion source system and its application. Background technique: [0002] Lipid is an important active substance in living organisms. It is not only the main constituent material of cell membrane, but also the medium molecule of cell energy storage and signal transmission. The emergence and development of Alzheimer's disease, atherosclerosis, diabetes and other diseases are closely related to abnormal lipid metabolism. The lipid composition of organisms is complex and diverse, consisting of thousands of molecules with different chemical structures, different physical and chemical properties, and different substance concentrations. Lipidomics is an important method to study the structure, composition and function of lipids in organisms, and it is also an important field of analytical chemistry and biological research in recent years. Chromatog...

Claims

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Application Information

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IPC IPC(8): G01N27/62G01N1/34G01N1/40H01J49/16
CPCG01N27/62G01N1/4055G01N1/34H01J49/167G01N2001/4061
Inventor 杨运云邓洁薇何凯丽向章敏
Owner GUANGDONG INST OF ANALYSIS CHINA NAT ANALYTICAL CENT GUANGZHOU