Hematoxylin dye liquor as well as preparation method and application method thereof

A technology of hematoxylin and dyeing methods, applied in the preparation of test samples, etc., can solve the problems of cumbersome methods and uneven quality, and achieve the effects of short operation time, good dyeing quality, and simple operation steps

Inactive Publication Date: 2020-03-24
SUZHOU CANCERCELL BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The quality of the existing hematoxylin staining solution is uneven, and the use method is relatively cumbersome, which causes more inconvenience to the user

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Prepare hematoxylin stain:

[0035] Mix 100 parts of absolute ethanol, 24 parts of glacial acetic acid and 4 parts of hematoxylin to obtain liquid a;

[0036] Heat 400 parts of distilled water and 22 parts of potassium aluminum sulfate to 90°C, mix well to obtain liquid b;

[0037] Mix liquid a, liquid b, and 0.4 parts of sodium iodate evenly, cool to room temperature under running water, then add 40 parts of glycerin, and shake for 1 hour.

[0038] Tissue staining: use brain tissue.

[0039] The tissue is frozen and sectioned, then placed in a fixative for fixation, and the fixed tissue section is washed to remove excess fixative;

[0040] The washed tissue sections were stained with the hematoxylin staining solution for 2-4 minutes, washed with water after staining, the washed tissue sections were differentiated with hydrochloric acid ethanol for 1-5 seconds, and then washed with water, and the washed tissue sections were turned blue with dilute ammonia for 10 secon...

Embodiment 2

[0042] Prepare hematoxylin stain:

[0043] Mix 80 parts of absolute ethanol, 28 parts of glacial acetic acid and 2 parts of hematoxylin to obtain liquid a;

[0044] Heat 600 parts of distilled water and 16 parts of potassium aluminum sulfate to 95°C, mix well to obtain liquid b;

[0045] Mix liquid a, liquid b, and 0.1 part of sodium iodate evenly, cool to room temperature under running water, then add 60 parts of glycerin, and shake for 1 hour.

[0046] Tissue staining: use brain tissue.

[0047] The tissue is frozen and sectioned, then placed in a fixative for fixation, and the fixed tissue section is washed to remove excess fixative;

[0048] The washed tissue sections were stained with the hematoxylin staining solution for 2-4 minutes, washed with water after staining, the washed tissue sections were differentiated with hydrochloric acid ethanol for 1-5 seconds, and then washed with water, and the washed tissue sections were turned blue with dilute ammonia for 10 seconds...

Embodiment 3

[0050] Prepare hematoxylin stain:

[0051] Mix 90 parts of absolute ethanol, 26 parts of glacial acetic acid and 3 parts of hematoxylin to obtain liquid a;

[0052] Heat 500 parts of distilled water and 19 parts of potassium aluminum sulfate to 90°C, mix well to obtain liquid b;

[0053] Mix liquid a, liquid b, and 0.25 parts of sodium iodate evenly, cool to room temperature under running water, then add 50 parts of glycerin, and shake for 1 hour.

[0054] Tissue staining: use brain tissue.

[0055] The tissue is frozen and sectioned, then placed in a fixative for fixation, and the fixed tissue section is washed to remove excess fixative;

[0056] The washed tissue sections were stained with the hematoxylin staining solution for 3 minutes, washed with water after staining, and the washed tissue sections were differentiated with hydrochloric acid ethanol for 3 seconds, and then washed with water. The washed tissue sections were turned blue with dilute ammonia water for 10 sec...

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PUM

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Abstract

The invention discloses hematoxylin dye liquor as well as a preparation method and an application method thereof. The preparation method of the hematoxylin dye liquor comprises the following steps ofmixing 80-100 parts of absolute ethyl alcohol, 24-28 parts of glacial acetic acid and 2-4 parts of hematoxylin to obtain liquor a; heating and uniformly mixing 400-600 parts of distilled water and 16-22 parts of aluminum potassium sulfate to obtain a solution b; and uniformly mixing the solution a, the solution b and 0.1-0.4 part of sodium iodate, cooling to room temperature by the flowing water,then adding 40-60 parts of glycerol, and oscillating for 1 hour. A staining method comprises the following steps of freezing and slicing the tissues, then putting the frozen and sliced tissues into astationary liquid to be fixed, and washing the fixed tissue slices with water; then dyeing with the hematoxylin dye liquor, washing with water, differentiating with hydrochloric acid ethanol, washingwith water, returning to blue for 10s with dilute ammonia water, washing with water, slightly washing with 95 wt% ethanol, dehydrating with absolute ethyl alcohol, transparentizing with xylene, and sealing with neutral gum. The dyeing time is only 2-4 minutes, the use is convenient, the dyeing effect is better, and the dyed cell nucleus is bright blue and bright.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a hematoxylin dye solution, a preparation method and an application method thereof. Background technique [0002] Hematoxylin is a staining agent extracted from Hematoxylin wood, which is oxidized to produce hematoxylin, which is widely used in the staining of tissue sections or cultured cells. Hematoxylin is a basic dye, which mainly makes the chromatin in the nucleus and the ribosome in the cytoplasm purple blue, and the mature hematoxylin staining solution is purple red. [0003] Hematoxylin staining is the most commonly used staining method in tissue sections. There are many formulations of hematoxylin staining solutions, and the staining effects are also different. There are many factors that affect the staining effect. The quality of the existing hematoxylin staining solution is uneven, and the use method is relatively cumbersome, which causes more inconvenience to the user. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/30
CPCG01N1/30
Inventor 王守立赵飞沈刚
Owner SUZHOU CANCERCELL BIOTECH
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