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A Colorimetric Sensing Method Based on Terephthalaldehyde Acetal 2-Amino-4-Methylphenol Schiff Base for the Detection of Dopamine

A technology of terephthalaldehyde and methyl phenol, which is applied in the measurement of color/spectral properties, preparation of test samples, etc., can solve the problems of complex pretreatment process, inconvenient and sensitive detection of dopamine, low sensitivity, etc., and achieve good application Prospect, low cost, high sensitivity effect

Active Publication Date: 2022-03-18
FUJIAN MEDICAL UNIV
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Problems solved by technology

However, these methods require complex pretreatment processes, and their sensitivity is low, making it difficult to quickly and sensitively detect dopamine.

Method used

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  • A Colorimetric Sensing Method Based on Terephthalaldehyde Acetal 2-Amino-4-Methylphenol Schiff Base for the Detection of Dopamine
  • A Colorimetric Sensing Method Based on Terephthalaldehyde Acetal 2-Amino-4-Methylphenol Schiff Base for the Detection of Dopamine
  • A Colorimetric Sensing Method Based on Terephthalaldehyde Acetal 2-Amino-4-Methylphenol Schiff Base for the Detection of Dopamine

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Embodiment Construction

[0017] The present invention is described in detail below in conjunction with accompanying drawing and embodiment:

[0018] Method among the present invention, specifically comprises the following steps:

[0019] (1) Visible light-induced oxidase-like activity of MAPTPA

[0020] Light-induced simulation of terephthalaldehyde acetal 2-amino-4-methylphenol Schiff base (MAPTPA) using 3,3',5,5'-tetramethylbenzidine (TMB) as chromogenic substrate Enzyme activity was studied. In 400 μL of 10 mM NaAc-HAc (pH = 4.0) buffer system, add 10 μL of 2.5 mM MAPTPA and 25 μL of mM TMB. After the solutions were mixed evenly at room temperature, they were placed under visible light for 6 min. Finally, the ultraviolet-visible absorption spectra were measured with a microplate reader. The visible light source in this experiment comes from a 300 W xenon lamp (λ≥400 nm). Such as figure 1 As shown, under the irradiation of visible light, phenol base can catalyze the oxidation of TMB to turn it...

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Abstract

The invention discloses a MAPTPA-based colorimetric sensing method for the detection of dopamine, which is characterized in that 50 μM MAPTPA is used as a visible light-induced oxidase, 500 μM TMB is used as a chromogenic substrate, and 500 μL of 10 mM pH = 4.0 NaAc-HAc buffer solution, and different concentrations of dopamine were added to the above solution, after mixing, they were illuminated with visible light for 6 min, and finally, the absorbance change value at 652 nm was measured with a microplate reader. With the increase of dopamine concentration, the absorbance value gradually decreases. In the concentration range of 0.01-100 μM, the absorbance and the logarithm of the dopamine concentration are linearly related, while the normal concentration of dopamine in human blood is 1.06-2.11 μM, which shows that the present invention can be applied Based on the actual clinical dopamine detection, a simple, stable, economical and rapid colorimetric sensing method was constructed.

Description

technical field [0001] The invention relates to a colorimetric sensing method based on terephthalaldehyde acetal 2-amino-4-methylphenol Schiff base (MAPTPA) for the detection of dopamine, adding different concentrations of dopamine to the prepared MAPTPA and TMB solution Among them, the absorbance value at 652 nm is detected, and the corresponding concentration is obtained by using the obtained working curve, so as to achieve the purpose of quantitative detection of dopamine, which belongs to the field of analytical chemistry and sensing materials. Background technique [0002] Dopamine (Dopamine, DA, 3,4-dihydroxy-β-phenylethylamine), a catecholamine neurotransmitter, plays an important role in the central nervous system, cardiovascular system, kidney system and endocrine system. It is a precursor for the biosynthesis of norepinephrine or adrenaline in the body and an important transmitter of information in the brain. The normal mass concentration of dopamine in human bloo...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/31G01N1/38
CPCG01N21/31G01N1/38
Inventor 韩志钟翁清花黄思琦陈舒昱王家烨尚兴王飞康杰
Owner FUJIAN MEDICAL UNIV
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