A method, device and control system for insulin release and recovery
A recovery system, insulin technology, applied in the field of insulin release and recovery, to reduce the frequency, increase availability, and avoid damage
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Embodiment 1
[0044] Example 1: Preparation method of magnetically labeled insulin
[0045] In the present invention, we used EDC / NHS to activate the surface carboxyl group-modified MNPs to react with the amino group of insulin, and achieved good results. Below is our method for labeling insulin with nanomagnetic particles.
[0046] (1) Dissolve 8 mg of EDC and 1 mg of NHS in 2 mL of deionized water, add 2 mL of carboxylated ferric oxide nanoparticles, and stir at room temperature for 20 min.
[0047] (2) Put the stirred small beaker or small centrifuge tube containing the mixed solution on a magnet (or magnetic stirrer) to help the magnetic nanoparticles to precipitate, then pour out the supernatant, add deionized water to wash, Repeat this 2-3 times.
[0048] (3) Add the washed nano-magnetic particles to 5 mL of insulin solution (insulin solution preparation: 5 mg of insulin is dissolved in 1 mL of 0.01 mol / L hydrochloric acid, and then diluted to 5 mL with PBS pH 7.4 - if any precipita...
Embodiment 2
[0054] like Figure 4 As shown, an insulin recovery and release device includes a recovery system I, a magnetic separation system II and a magnetically labeled insulin storage chamber III,
[0055] The recovery system 1 includes: an insulin enrichment tube 3 and an electromagnetic coil I2 arranged around the insulin enrichment tube 3;
[0056] The magnetic separation system II includes: a Y-shaped pipeline, which includes an A inlet 5, a B outlet 7 and a C outlet 8, and a permanent magnet 6 is provided on one side of the B outlet 7 of the Y-shaped pipeline;
[0057] The magnetically labeled insulin storage chamber III comprises: a housing 15, a piston 12 is arranged in the housing 15, an electromagnetic coil III10 is arranged at the left end, and a micromotor 11 is arranged at the right end, and the piston 12 divides the inner space of the housing 15 into a left chamber 17 and a left chamber. The right chamber 18, the piston 12 is provided with a reversing valve 9 that commun...
Embodiment 3
[0061] like Figure 5 As shown, a control system for an insulin recovery and release device includes:
[0062] Blood sugar sensor, used to detect blood sugar concentration in blood;
[0063] The controller is used to receive the blood sugar concentration signal of the blood sugar sensor. When the blood sugar concentration is higher than the set threshold, the electromagnetic coil I and the electromagnetic coil III are not energized, start the micromotor, make the piston reciprocate, and release the magnetically labeled insulin; When the blood glucose concentration is lower than the set threshold, the electromagnetic coil I and the electromagnetic coil III are energized, the micromotor is started, the piston reciprocates, and the magnetically labeled insulin is recovered; when the blood glucose concentration is normal, the electromagnetic coil I and the electromagnetic coil III are not energized, Turn off the micromotor.
[0064] The working principle of the device for insuli...
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Abstract
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