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A kind of cancer biomarker, use

A biomarker and cancer technology, applied in the field of tumor molecular biology, can solve the problems of delaying the best treatment time for patients, increasing the risk, increasing the pain of patients, etc., and achieve the effect of reducing treatment risk, prolonging life, and reducing pain

Active Publication Date: 2021-07-16
SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Therefore, the technical problem to be solved by the present invention is to overcome that there is no predictive biomarker for PD-1 antibody treatment of tumors in the prior art, and it is impossible to evaluate tumor patients who may not be suitable for PD-1 antibody treatment, resulting in tumor The risk of patients being treated with PD-1 antibody increases, which increases the suffering of patients and easily delays the best treatment opportunity for patients, and then proposes a cancer biomarker that can be used to predict, evaluate or identify PD -1 antibody is effective in the treatment of tumor patients, predicts tumor patients who are not suitable for PD-1 antibody treatment, provides more effective medication and treatment options for cancer patients, reduces the risk of treatment for tumor patients, reduces the suffering of patients, and prolongs patient life

Method used

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  • A kind of cancer biomarker, use
  • A kind of cancer biomarker, use
  • A kind of cancer biomarker, use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] In this example, the RT-PCR method was used to detect the mRNA levels of PD-1 in 12 cancer types, including 35 cell lines.

[0060] Cancer cells are: blood cancer cells include Raji, K-562, Jeko-1, U-937 and Jurkat; breast cancer cells include MCF-7, SK-BR-3 and T47D; intestinal cancer cells include HT-29 , RKO, SW480, and HCT116; pancreatic cancer cells include HPDE6C, BxpC-3, and AsPC-1; prostate cancer cells include PC-3, 22Rv1, DU145, and LNCaP; bone cancer cells include CRL8303 or U-2OS; Cells for skin cancer include ACHN, 769-P, 786-O, and Caki-1; cells for skin cancer include B16F10 and A-375; cells for cervical cancer include Ca Ski and Hela; cells for brain cancer include M059J, SKNSH, M059K, and SK-N-BE(2); liver cancer cells include Huh-7 and HepG2; gastric cancer cells include HGC-27. The above-mentioned cancer cells are all commercially available products.

[0061] Described RT-PCR method comprises the steps:

[0062] 1. Extraction of total cellular RNA ...

Embodiment 2

[0085] In this example, the fluorescent real-time quantitative PCR (Q-PCR) technology was used to detect the mRNA levels of PD-1 in 12 cancer types, including 35 cell lines.

[0086] The cancer cells are as the cancer cells in Example 1.

[0087] Described Q-PCR method comprises the steps:

[0088] 1. Q-PCR primers, the primers are as follows in Table 3:

[0089] Table 3 Primers

[0090]

[0091] 2. Q-PCR reaction system: SYBR Green dye (Takara) was used, and each cDNA was tested in three multiple wells. Each well was prepared according to the reaction system in Table 4 below. The cDNA in Table 4 was RT in Example 1. -Reaction solution containing cDNA obtained by PCR:

[0092] Table 4 reaction system

[0093]

[0094] 3. Q-PCR reaction program: 95°C, 10min; 35 cycles: 95°C, 15s; 60°C, 1min.

[0095] The results of Q-PCR test are attached Figure 1B , Figure 1B The ordinate in the graph represents the Delta CT value (the CT value of the target gene minus the CT va...

Embodiment 3

[0097] In this example, immunoblotting (Immunoblot) technology, RT-PCR and fluorescent real-time quantitative PCR were used to detect the expression of PD-1 in four lung cancer cell lines.

[0098] The four lung cancer cell lines are A549, HCC827, Calu-1 and NCI-H1299. The above-mentioned cancer cells are all commercially available products.

[0099] 1. Western blot detection of PD-1 expression in four lung cancer cell lines

[0100] Including the following steps:

[0101] (1) Protein extraction: Observe the cell state and density under a microscope. After meeting the experimental requirements, remove the supernatant, wash it once with PBS, remove the PBS, blot dry as much as possible, add an appropriate amount of cell lysate, and gently scrape the cells with a cell scraper. Scrape off from the bottom of the dish, then transfer the scraped cells to an EP tube, lyse on ice for 20min, then centrifuge at 12,000rpm at 4°C for 20min, take the supernatant, and set aside.

[0102]...

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Abstract

The present invention relates to the field of tumor molecular biology, in particular to a cancer biomarker and its use, the cancer biomarker including programmed death molecule PD‑1, gene PDCD1 and / or PD‑1 mRNA in tumor cells; PD‑1 1 is widely expressed in tumor cells and plays a role in inhibiting the growth of tumor cells. The above-mentioned cancer biomarkers are used to predict, evaluate or identify the effectiveness of PD-1 antibody treatment for immunodeficiency or immunocompromised tumor patients, predict tumor patients who are not suitable for PD-1 antibody treatment, and provide tumor patients with Suggestions for more effective medication and therapy selection provide important warnings for the clinical application of immunotherapy in the future, reduce the risk of treatment for tumor patients, reduce the pain of patients, and prolong the life of patients. A predictive biomarker for tumors treated with PD‑1 antibodies, unable to assess issues in patients with tumors who may not be eligible for PD‑1 antibody therapy.

Description

technical field [0001] The invention relates to the field of tumor molecular biology, in particular to a cancer biomarker and its application. Background technique [0002] Programmed cell death protein 1 (PD-1), a type I transmembrane glycoprotein, is an important negative regulator of immune response. Under normal circumstances, PD-1 inhibits the function of T lymphocytes by binding to its ligands PD-L1 and PD-L2, thereby inhibiting the autoimmune response. The therapeutic mechanism of PD-1 antibody and PD-L1 antibody is to bind to PD-1 on the surface of T cells and PD-L1 on the surface of tumor cells respectively, so that PD-L1 on the surface of tumor cells cannot bind to PD-1 on the surface of T cells. T cells are then activated to eventually recognize and kill tumor cells. Currently, immune checkpoint blocker anti-PD-1, anti-PD-L1 therapeutic antibodies have been used to treat a variety of human tumors, such as melanoma, renal cell carcinoma, NSCLC and Hodgkin lymphom...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68G01N33/574C12Q1/6886A61K45/00A61P35/00
CPCA61K45/00A61P35/00C12Q1/6886C12Q2600/106G01N33/57407G01N33/57415G01N33/57419G01N33/57423G01N33/57426G01N33/5743G01N33/57434G01N33/57438G01N33/57442G01N33/68G01N2333/70521
Inventor 高山王晓冬
Owner SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
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