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Composite hydrogel for promoting osteomyelitis healing and preparation method thereof

A composite hydrogel and osteomyelitis technology, applied in the field of biomedical engineering materials, to achieve the effect of shortening the treatment cycle, reducing physical and economic burden, and controlling infection

Active Publication Date: 2020-03-31
BEOGENE BIOTECH GUANGZHOU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are currently few reports of matrix systems that can both effectively control infection and achieve effective bone regeneration without additional surgery

Method used

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  • Composite hydrogel for promoting osteomyelitis healing and preparation method thereof
  • Composite hydrogel for promoting osteomyelitis healing and preparation method thereof
  • Composite hydrogel for promoting osteomyelitis healing and preparation method thereof

Examples

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Effect test

preparation example Construction

[0041] The embodiment of the present invention provides a method for preparing a composite hydrogel that promotes the healing of osteomyelitis, and the specific steps are as follows:

[0042] (1) CMCS and HA are mixed according to the mass ratio of (0.5~2.0):1, and dissolved in PBS solution to obtain a mixed solution A with a mass fraction of 1.5~2.5wt%;

[0043] (2) Add bone morphogenetic protein sustained-release microspheres and antibiotics to PBS solution at 4°C to obtain mixed solution B; the concentration of antibiotics in mixed solution B is 60-150 mg / mL, and the concentration of bone morphogenetic protein sustained-release microspheres The concentration is 1.5~2.5μg / mL;

[0044] (3) The mixed solution A and the mixed solution B were mixed at a volume ratio of 1:1, then genipin was added in an amount of 50 μg / mL, left at room temperature for 4-6 hours for cross-linking, and the composite hydrogel was prepared.

[0045] The preparation method of the bone morphogenetic p...

Embodiment 1

[0055] Preparation of bone morphogenetic protein sustained-release microspheres (hereinafter referred to as PLGA@BMP-2 sustained-release microspheres):

[0056] (1) Dissolve 0.25g of PVA in 250mL of deionized water, stir at 70°C until the PVA is completely dissolved and then cool to room temperature to obtain a PVA solution;

[0057] (2) Weigh 0.3g PLGA and dissolve it in 3mL of dichloromethane to obtain PLGA solution;

[0058] (3) Dissolving BMP-2 in PBS solution to make 1.0 μg / mL BMP-2 solution;

[0059] (4) Take 500 μL of BMP-2 solution and drop it into 3 mL of PLGA solution to obtain mixed solution C;

[0060] (5) PVA solution and mixed solution C are put into respectively in the beaker that fills ice cube and carry out ice bath and make it cool down;

[0061] (6) Take 500 μL of PBS solution and add it to the mixed solution C, and use an ultrasonic cell disruptor to ultrasonically disperse for 15 seconds at a power of 200 W until the liquid is milky white to obtain a col...

Embodiment 2

[0067] Preparation of CMCS(0.5) / HA(1) / PLGA@BMP-2 hydrogel:

[0068] (1) CMCS and HA are mixed in a mass ratio of 0.5:1, dissolved in a PBS solution, to obtain a mixed solution A with a mass fraction of 2.0 wt %;

[0069] (2) Prepare a mixed solution B with a concentration of 2.0 μg / mL with cold PBS solution (4° C.) and the PLGA@BMP-2 sustained-release microspheres of Example 1;

[0070] (3) Mix the mixed solution A and the mixed solution B at a volume ratio of 1:1, then add genipin in an amount of 50 μg / mL, transfer 400 uL of the uniform liquid into a 48-well plate, place it at room temperature for 5 hours, CMCS(0.5) / HA(1) / PLGA@BMP-2 hydrogel was obtained.

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Abstract

The invention discloses a composite hydrogel for promoting osteomyelitis healing, and a preparation method of the composite hydrogel comprises the following steps: mixing a mixed solution A containingcarboxymethyl chitosan and hyaluronic acid with a mixed solution B containing antibiotics and bone morphogenetic protein sustained-release microspheres, adding a crosslinking agent, and crosslinkingto obtain the composite hydrogel. The composite hydrogel disclosed by the invention is of a uniform porous reticular structure, can effectively promote wound healing, and has good bionic performance,biocompatibility, mechanical property and degradability. Moreover, the composite hydrogel disclosed by the invention can continuously release antibacterial antibiotics for a long time and induce bonemorphogenetic protein formed by new bones, infection can be effectively controlled, bone regeneration can be effectively promoted under the condition that no extra operation is conducted, the operation frequency is reduced, the treatment period is shortened, the physical and economic burdens of a patient are relieved, and the composite hydrogel has a good application prospect in osteomyelitis treatment.

Description

technical field [0001] The invention relates to the technical field of biomedical engineering materials, in particular to a composite hydrogel for promoting healing of osteomyelitis and a preparation method thereof. Background technique [0002] Osteomyelitis, a bacterial infection of the bone or bone marrow, is a problem for orthopedic surgeons. Hip prosthesis, knee replacement, environmental exposure fractures, orthopedic surgery, and soft tissue infection are major risk factors for osteomyelitis. Although healthy human bones are highly resistant to infection, the incidence of osteomyelitis is gradually increasing in the immunocompromised elderly population with osteoarthritis, rheumatoid arthritis, osteoporosis, etc. Bacteria within the lesion release osteolytic cytokines and osteonecrosis factors, causing severe pain and bone loss. [0003] In recent years, local drug delivery systems based on polymethylmethacrylate (PMMA)-based bone binders containing antibiotics have...

Claims

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Application Information

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IPC IPC(8): A61L27/20A61L27/52A61L27/54A61L27/56A61L27/58
CPCA61L27/20A61L27/52A61L27/54A61L27/56A61L27/58A61L2430/02A61L2300/406A61L2300/414A61L2300/602A61L2300/622C08L5/08
Inventor 王梦颖冯龙宝蓝咏刘玉
Owner BEOGENE BIOTECH GUANGZHOU
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