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Rhodopseudomonas palustris high-density fermentation method and application thereof

A swamp rhodopseudomonas, high-density fermentation technology, applied in the field of microorganisms, can solve the problems that products cannot be guaranteed from contamination by miscellaneous bacteria, product quality cannot be guaranteed, etc., and is conducive to mass production, low cost, and high social benefits. Effect

Pending Publication Date: 2020-04-10
XINJI DAHE CHEM CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this type of method cannot guarantee that the product will not be contaminated by miscellaneous bacteria, and production will be affected under cloudy or low temperature conditions. The bacterial density of the fermentation liquid is not higher than 5 billion / mL, and the quality of the final product cannot be guaranteed.

Method used

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  • Rhodopseudomonas palustris high-density fermentation method and application thereof
  • Rhodopseudomonas palustris high-density fermentation method and application thereof
  • Rhodopseudomonas palustris high-density fermentation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0050] Embodiment 1 prepares activated seed solution

[0051] 1) Preparation of liquid seed medium. Accurately weigh each component according to the ratio of each component in the liquid seed culture medium described in the claims, sodium acetate 10g / L, yeast powder 10g / L, dipotassium hydrogen phosphate 1.0g / L, magnesium sulfate heptahydrate 0.5 g / L, adjust the pH to 7.0-8.0, and sterilize at 121°C for 30 minutes.

[0052] 2) Activate the seed solution. The production seeds were added to this medium in an amount of 1%, and cultivated in a constant temperature shaker at a temperature of 28° C., a humidity of 60%, and a shaker speed of 120 rpm. After culturing for 3 days, the activated seed solution was obtained, such as figure 1 shown.

Embodiment 2

[0053] Embodiment 2 prepares a kind of liquid

[0054] 1) Accurately weigh each component according to the proportioning of each component in the liquid seed medium according to the claim, sodium acetate 10g / L, yeast powder 10g / L, dipotassium hydrogen phosphate 1.0g / L, sulfuric acid heptahydrate Magnesium 0.5g / L, adjust the pH to 7.0-8.0, and sterilize at 121°C for 30 minutes.

[0055] 2) Inoculate the activated seed solution into a primary fermenter equipped with a liquid seed medium, and cultivate it for 3 days at a constant temperature of 28°C, stirring at 120rpm, and with a ventilation rate of 1:0.5, to obtain the primary fermenter of Rhodopseudomonas palustris. kind of liquid, such as figure 2 shown.

Embodiment 3

[0056] Embodiment 3 high-density fermentation

[0057] 1) Accurately weigh each component according to the proportioning of each component in the liquid fermentation medium described in the claim, sodium acetate 30g / L, yeast powder 10g / L, ammonium sulfate 10g / L, dipotassium hydrogen phosphate 2.0g / L, magnesium sulfate heptahydrate 1.0g / L, ferrous sulfate heptahydrate 0.4g / L, adjust the pH to 7.0-8.0, and sterilize at 121°C for 30 minutes.

[0058] 2) Accurately weigh each component according to the proportioning of each component in the liquid feed medium described in the claim, sodium acetate 300g / L, yeast powder 100g / L, ammonium sulfate 100g / L, and adjust pH 5.0- 8.0, sterilized at 121°C for 30 minutes.

[0059] 3) Inoculate all the primary seed liquid into a fermenter equipped with a liquid fermentation medium, and cultivate it for 5 days at a constant temperature of 28°C, stirring at 120rpm, and ventilation at a rate of 1:0.5. Feed the culture medium to finally obtain a...

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Abstract

The invention belongs to the technical field of microorganisms, and relates to a rhodopseudomonas palustris high-density fermentation method and an application thereof. The rhodopseudomonas palustrisstrain is sourced from China General Microbiological Culture Collection Center, and a preservation number is CICC 23812. According to the rhodopseudomonas palustris high-density fermentation method, shake flasks are sequentially adopted for seed activation, the activated shake flask seed liquid is inoculated into a first-stage fermentation tank for expanding culture to prepare first-stage seeds, the first-stage seeds are transferred into a second-stage fermentation tank for fermentation production, fed-batch feeding is employed in the fermentation process to improve the bacterial density of the fermentation liquid, and the bacterial density can at least reach 50 billion CFU / ml after fermentation for 5 days. The problems that in the prior art, illumination is needed, the period is long, theinfectious microbe rate is high, and the product quality is not easy to control are solved. The method has the advantages of short fermentation period, stable product quality, low production cost andthe like. The rhodopseudomonas palustris fermentation liquor obtained by the method can be further processed into a micro-ecological feed additive, a microbial fertilizer and a microbial water purifying agent.

Description

technical field [0001] The invention relates to a high-density fermentation method of Rhodopseudomonas palustris and an application thereof, belonging to the technical field of microbes. Background technique [0002] Rhodopseudomonas palustris (Rhodop seudomonas palustris) is a photosynthetic bacterium that is widely studied and used. At the same time, it can secrete a variety of beneficial substances, such as B vitamins, coenzyme Q and carotenoids. In view of the above characteristics, the application of Rhodopseudomonas palustris in wastewater treatment, aquaculture, agriculture and animal husbandry has great potential. Irreplaceable biological effects, especially in recent years, the abuse of antibiotics, excessive use of pesticides and fertilizers, and poor wastewater treatment have emerged one after another. The green and environmental protection of Rhodopseudomonas palustris as a biological product has attracted more attention from people from all walks of life. [00...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23K10/18C05F11/08C02F3/34C12R1/01
CPCC12N1/20A23K10/18C05F11/08C02F3/34
Inventor 刘伯雅何立肖
Owner XINJI DAHE CHEM CO LTD
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