Biotransformation method for efficient synthesis of gamma-aminobutyric acid
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A technology of aminobutyric acid and biotransformation, applied in the field of fermentation engineering, can solve the problems such as the yield to be improved, and achieve the effects of simple ingredients, good culture effect, and increased GABA output
Active Publication Date: 2020-04-10
NANCHANG UNIV
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[0005] The purpose of the present invention is to address the technical defects of the prior art and provide a biotransformation method for efficiently synthesizing γ-aminobutyric acid to solve the problem that the yield of conventional lactic acid bacteria strains and conventional fermentation processes needs to be improved in the process of lactic acid bacteria fermentation to produce GABA technical problem
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Embodiment 1
[0022] After the activation of Lactobacillus brevis CD0817, it was transferred to the liquid seed medium, inoculated into the fermentation medium with a 10% inoculation amount, and cultured statically at 30° C. for 36 hours.
[0023] Seed medium (g / L) includes: yeast extract powder 25, glucose 40, L-sodium glutamate 28, Tween 80 1, MnSO 4 ·H 2 O 0.1 mM, pH 5.0.
[0024] Fermentation medium (g / L) includes: yeast extract powder 25, glucose 2.5, Tween 80 1.5, MnSO 4 ·H 2 O 0.2mM, L-glutamic acid 650. The fermented culture medium was tested, and the obtained GABA content reached 280g / L.
Embodiment 2
[0026] After the activation of Lactobacillus brevis CD0817, it was transferred to the liquid seed medium, inoculated into the fermentation medium with a 10% inoculation amount, and cultured statically at 35° C. for 36 hours.
[0028] Fermentation medium (g / L) includes: yeast extract powder 35, glucose 5, Tween 80 1.5, MnSO 4 ·H 2 O 0.4mM, L-glutamic acid 650. The fermented culture medium was tested, and the obtained GABA content reached 299g / L.
Embodiment 3
[0030] After the activation of Lactobacillus brevis CD0817, it was transferred to the liquid seed medium, inoculated into the fermentation medium with a 10% inoculation amount, and cultured statically at 32° C. for 36 hours.
[0032] Fermentation medium (g / L) includes: yeast extract powder 35, glucose 5, Tween 80 2, MnSO 4 ·H 2 O 0.6mM, L-glutamic acid 650. The fermented culture medium was tested, and the obtained GABA content reached 311g / L.
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Abstract
The invention provides a biotransformation method for efficient synthesis of gamma-aminobutyric acid. According to the technical scheme, Lactobacillus brevis CCTCC M2018462 selected in the early stageis used as a fermentation strain, and abnormally high GABA yield is achieved through the cooperation of the strain, culture medium and culture process. Specifically, the method comprises the following steps: firstly inoculating Lactobacillus brevis CCTCC M2018462 into a seed culture medium with specific components, cultivating in a shaker at a temperature of 30-35 DEG C for 7-9h so that the OD600value of the culture solution reaches 4-6 to obtain a seed culture solution; then inoculating 10% inoculum into a fermentation medium with specific components, standing and culturing at the temperature of 30-35 DEG C for 36-72 h. Therefore, high content of GABA is accelerated in the culture solution. Experimental verification shows that the GABA content in the lactic acid bacteria fermentation broth obtained by the invention is as high as 280-320 g/L, which is the highest level as ever reported. According to the invention, the output of GABA is significantly improved, and a good foundation islaid for large-scale application of lactic acid bacteria fermentation for production of GABA.
Description
technical field [0001] The invention relates to the technical field of fermentation engineering, in particular to a biotransformation method for efficiently synthesizing γ-aminobutyric acid. Background technique [0002] γ-Aminobutyric acid (GABA) is a natural non-protein amino acid that widely exists in animals, plants and microorganisms. As an important inhibitory neurotransmitter in the mammalian central nervous system, it participates in various physiological and biochemical reactions. It has various physiological functions such as diuresis, lowering blood pressure, anti-anxiety, invigorating the liver and kidney, promoting brain reflux, and nourishing nerve cells. [0003] At present, the synthesis methods of GABA mainly include chemical synthesis, animal and plant enrichment and microbial transformation. The chemical synthesis method involves toxic reagents, the reaction conditions are severe, and the safety is low; the animal and plant enrichment method has defects s...
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