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The method of increasing the content of secondary metabolites of black rice tree by infecting with Megacystis maxima

A technology of secondary metabolites and urchins, applied in the fields of soil microorganisms, environment, and food additives, can solve the problem of low content of secondary metabolites

Active Publication Date: 2022-03-11
宜兴乾元黑色食品科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the content of endogenous secondary metabolites in the leaves of Wufan tree grown in a natural state is low, and needs to be further improved.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] The root system of the black rice tree was sterilized by 0.01% mercury liter, washed with distilled water, soaked in 1 spore / ml G. gigantea strain culture solution for 0.01h, and the infected black rice tree was cultured in normal soil for 1 day, and the leaves were measured by HPLC method The content of orientin, a secondary metabolite of flavonoids, showed that the content of orientin increased by 0.01% compared with that before G. gigantea infection.

Embodiment 2

[0033] The root system of the black rice tree was sterilized by 0.25% KMnO4, washed with sterilized water, soaked in 250 spores / ml G. gigantea strain culture solution for 0.5h to infect, and the inoculated black rice tree was cultured in normal soil for 3 months Adopt HPLC method to measure the content of secondary metabolite 10-O-p-hydroxycinnamoyl-dihydrocrystal aglycone in its plant, the result shows that the content of 10-O-p-hydroxycinnamoyl-dihydrocrystal aglycon is higher than that of G . gigantea strains increased by 0.5% before infection.

Embodiment 3

[0035]Adopt 10 milliliters that contain 500 spores / ml G. gigantea bacterial classification culture solution, pour in the soil around the root of the black rice tree planted in soil to infect, after infecting 1 month, adopt HPLC method to measure the number of times in the black rice tree plant Raw metabolites vitexin, isorientin, orientin, quercetin-3-O-β-D-glucuronide, (trans) 10-O-p-hydroxycinnamoyl-dihydro crystal blue Aglycone, (cis) 10-O-p-hydroxycinnamoyl-dihydrocrystal aglycone, (trans) 10-O-p-hydroxycinnamoyl-chrysanthemum aglycon, (cis) 10-O - the content of p-hydroxycinnamoyl-galinagenin, the results showed that the content of vitexin increased by 0.1%, the content of isoorientin increased by 0.5%, the content of orientin increased by 0.3%, quercetin- The content of 3-O-β-D-glucuronide increased by 0.01%, the content of (trans) 10-O-p-hydroxycinnamoyl-dihydrocrystal aglycone increased by 0.02%, (cis) 10-O - The content of p-hydroxycinnamoyl-dihydrocrystal aglycone i...

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Abstract

The invention discloses a method for increasing the content of secondary metabolites in the black rice tree by infecting with Megacystis maxima. The present invention adopts Megacystis maxima to infect the root system of the black rice tree to form a symbiosis system of the black fan tree and the giant giant cyst G. gigantea. Through the mutual influence and mutual benefit and mutual assistance relationship between G. gigantea and the soil of the root system of the black rice plant, the content of secondary metabolites in the black rice plant is significantly increased. The invention can remarkably increase the content of secondary metabolites in the plant of the black rice plant, wherein the content of flavonoids is increased by no less than 0.01%, the content of iridoid glycosides is increased by no less than 0.01%, and the content of proanthocyanidins is increased by no less than 0.01%. At 0.01%, the content of other secondary metabolites increases by not less than 0.01%. The present invention can increase the content of iridoid glycosides, the functional components of black rice tree branches and leaves, and improve its coloring ability; it can also increase the content of flavonoids and proanthocyanidins secondary metabolites, thereby increasing the nutritional content of black rice .

Description

technical field [0001] The invention belongs to the field of soil microbes and the environment, and also belongs to the field of food additives, and relates to a method for increasing the content of secondary metabolites of acacia plants by infecting with Mycocystis maxima. [0002] technical background [0003] Gigaspora gigantea (hereinafter referred to as G. gigantea) is an Arbuscular mycorrhiza fungi. Arbuscular mycorrhizal fungi can form a symbiotic relationship with plants, but the infection of arbuscular mycorrhizal fungi is exclusive, and only when they establish symbiosis with the host plant can they complete their own life cycle. The existence of arbuscular mycorrhizal fungi plays an important role in the survival of host plants. It can significantly increase the adaptability of host plants to adversity and enhance their survival competitiveness. [0004] Black rice tree, also known as Nanzhu, is an evergreen shrub or tree of Rhododendronaceae and Vaccinium genus. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G17/00A01G7/06
CPCA01G17/005A01G7/06
Inventor 张琳付红伟
Owner 宜兴乾元黑色食品科技有限公司
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