Unlock instant, AI-driven research and patent intelligence for your innovation.

Chromosome inversion co-separated from peach fruit shape traits and application thereof

A technology of co-segregation and chromosome, which is applied in the field of plant molecular biology, can solve the problems of unclear genetic basis of peach flat fruit shape, and achieve the effects of improving breeding efficiency, saving breeding time, and rapid detection

Active Publication Date: 2020-04-17
WUHAN BOTANICAL GARDEN CHINESE ACAD OF SCI
View PDF3 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the genetic basis of peach flat fruit shape is still unclear and needs further in-depth research

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Chromosome inversion co-separated from peach fruit shape traits and application thereof
  • Chromosome inversion co-separated from peach fruit shape traits and application thereof
  • Chromosome inversion co-separated from peach fruit shape traits and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Example 1, Illumina next-generation resequencing library construction and data processing

[0052] Library preparation: First, high-quality genomic DNA was extracted from leaves using the CTAB method (Porebski et al. 1997). After passing the test, it was implemented according to the Illumina standard library construction protocol, including sample quality inspection, library construction, library quality inspection, and library sequencing. After the genomic DNA of the sample is qualified, the DNA is fragmented by mechanical fragmentation (ultrasonic wave), and then the fragmented DNA is purified, repaired, added A at the 3′ end, connected to the sequencing adapter, and then analyzed by agarose gel. Electrophoresis was used to select the size of the fragment, and PCR amplification was performed to form a sequencing library. The built library was first subjected to library quality inspection, and the library that passed the quality inspection was sequenced by Illumina. Th...

Embodiment 2

[0054] Example 2, Pacbio three-generation resequencing library construction and data processing

[0055] Library construction: Qualified DNA samples are randomly broken into fragments by the Covaris ultrasonic breaker; large fragments of DNA are enriched and purified by magnetic beads; fragmented DNA is repaired, damage repaired, and end repaired; stem-loops are connected at both ends of the DNA fragments The adapters were sequenced and exonucleases were used to remove fragments that failed to ligate. The constructed library was sequenced by Pacbio Sequel.

[0056] Data processing: Using MaSuRCA software, Illumina next-generation sequencing data was used to correct the PacBio third-generation data. Valid high-quality sequencing data were aligned to the reference genome by ngmlr software (parameter: -t 4). Use SAMTOOLs (mpileup-m 2-F 0.002-d 1000) to detect small fragment insertions and deletions (InDels) with a length less than 50 bp, and then use ANNOVAR software to annotat...

Embodiment 3

[0057] Example 3, Development of Molecular Markers for Chromosomal Inversions at S Site

[0058]According to the information of chromosomal inversion breakpoints at the S site, we designed three pairs of primers, P1F / P1R, P2F / P2R and P3F / P3R, for screening and identifying flat peach traits, which can amplify the regions of RefPB, PanPB and PanDB, respectively. The primer sequences are shown in SEQ ID NO.5, 6...10.

[0059] The PCR enzyme was selected from Qingke Biological Gold Medal Mix (green), the annealing temperature in the cycle was 58°C, and the extension time was 30s, a total of 35 cycles. After PCR is finished, use 1% agarose gel electrophoresis.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses chromosome inversion co-separated from peach fruit shape traits and an application thereof, which relate to a plant molecular biology technology. The nucleotide sequence near the reverse near breakpoint of the chromosome co-separated from the peach fruit shape traits is shown as SEQ ID NO.1, and the nucleotide sequence near the far breakpoint is shown as SEQ ID NO.2. Molecular markers are designed by utilizing nucleotide sequences on two sides of a reverse breakpoint and are used for marker-assisted screening in breeding, and fruit shape traits can be screened in advance. Wherein the sequences of the primers are shown as SEQ ID NO.5 to No.10. The invention has the following advantages and positive effects: (1) the S-locus large-fragment chromosome inversion disclosed by the invention is more likely to be a key reason for causing flat mutation of peach fruit shapes; (2) the molecular marker containing three pairs of primers can be used for accurately judging or predicting individual fruit shape traits; and (3) the chromosome inversion for controlling the flat fruit shape is used for marker-assisted selection to guide cross breeding of peaches, screening can be performed in advance, the breeding time is saved, and the breeding efficiency is improved.

Description

technical field [0001] The invention relates to plant molecular biology technology, which belongs to the study of genome structure variation and its influence on plant phenotype, and in particular relates to a chromosome inversion co-segregated with peach fruit shape traits and its application. Background technique [0002] Peach is one of the most important fruit trees in the world. It originated in the southwestern Qinghai-Tibet Plateau of my country about 2.5 million years ago (Yu Y. et al., Genome re-sequencing reveals the evolutionary history of peachfruit edibility. Nat Commun 2018; 9 :5404). The cultivation and domestication of peaches in my country can be traced back at least 4,000 years ago (Scorza R. and Okie W.R., Peaches (Prunus). Acta Hortic 1991, 290:177-234), and then spread to Europe along the Silk Road during the Roman period. It has become one of the important economic fruit trees in the temperate zone. The external fruit quality traits of peach have wide v...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/11C12Q1/6895
CPCC12Q1/6895C12Q2600/13C12Q2600/156Y02P60/87
Inventor 韩月彭周晖廖燎郑蓓蓓
Owner WUHAN BOTANICAL GARDEN CHINESE ACAD OF SCI