Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

High glucoraphanin and sulforaphane synthesis and releasing cultivation method for Brassica oleracea L.var.italic Planch. hairy roots

A technology of sulforaphane and a culture method is applied in the field of broccoli hairy root culture system to achieve the effects of promoting transformation rate, reducing feedback inhibition effect and good antioxidant capacity

Active Publication Date: 2020-04-21
GANSU AGRI UNIV
View PDF12 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Melatonin has been widely used and studied as a new plant hormone, but there is no report of melatonin being used in hairy root culture system

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • High glucoraphanin and sulforaphane synthesis and releasing cultivation method for Brassica oleracea L.var.italic Planch. hairy roots
  • High glucoraphanin and sulforaphane synthesis and releasing cultivation method for Brassica oleracea L.var.italic Planch. hairy roots
  • High glucoraphanin and sulforaphane synthesis and releasing cultivation method for Brassica oleracea L.var.italic Planch. hairy roots

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1 Broccoli hairy root glucoraphanin and sulforaphane synthesis and release culture method:

[0031] PCR detection of genes in broccoli hairy roots after sterilization rol B and rol C , choose to exist rol B and rol C The hairy root of the gene was used as the raw material, and the raw material was inoculated in the MS liquid medium containing methionine at pH = 6 and the concentration was 0.1 mg / L according to the inoculum amount of 0.5 g / L. / min constant temperature shaking culture for 14 days, and then add melatonin solution with a concentration of 10 mmol / L according to the dosage of 20 μmol / L. After 18 days of culture, it was detected that the biomass of hairy roots in each bottle (300 mL) was 4.5 g dry weight, The multiplication factor was 50.7, the contents of glucoraphanin and sulforaphane in hairy roots were 0.3 mg / g dry weight and 0.1 mg / g dry weight respectively, and the contents of glucoraphanin and sulforaphane in the medium were 61.5 mg / bott...

Embodiment 2

[0040] Example 2 Cultivation method for the synthesis and release of glucoraphanin and sulforaphane in broccoli hairy roots: the sterilized broccoli hairy roots are subjected to PCR detection of genes rol B and rol C , choose to exist rol B and rol C The hairy root of the gene was used as the raw material, and the raw material was inoculated in the MS liquid medium containing methionine at pH = 6 and the concentration was 0.5 mg / L according to the inoculum amount of 0.5 g / L. / min constant temperature shaking culture for 16 days, and then add melatonin solution with a concentration of 10 mmol / L according to the dosage of 60 μmol / L. After 18 days of culture, it was detected that the biomass of hairy roots in each bottle (300 mL) was 6.2 g dry weight, The multiplication factor is 70.4, the contents of glucoraphanin and sulforaphane in the hairy root are 0.2 mg / g dry weight and 0.3 mg / g dry weight respectively, and the contents of glucoraphanin and sulforaphane in the medium a...

Embodiment 3

[0043] Example 3 Cultivation method for the synthesis and release of glucoraphanin and sulforaphane in broccoli hairy roots: the sterilized broccoli hairy roots are subjected to PCR detection of genes rol B and rol C , choose to exist rol B and rol C The hairy root of the gene was used as the raw material, and the raw material was inoculated in the MS liquid medium containing methionine at pH = 6 and the concentration was 0.3 mg / L according to the inoculum amount of 0.5 g / L. / min constant temperature shaking culture for 15 days, and then add melatonin solution with a concentration of 10 mmol / L according to the dosage of 40 μmol / L. After 18 days of culture, it was detected that the biomass of hairy roots in each bottle (300 mL) was 3.4 g dry weight, The multiplication factor is 40.3, the contents of glucoraphanin and sulforaphane in the hairy root are 0.2 mg / g dry weight and 0.2 mg / g dry weight respectively, and the contents of glucoraphanin and sulforaphane in the medium a...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a high glucoraphanin and sulforaphane synthesis and releasing cultivation method for Brassica oleracea L.var.italic Planch. hairy roots. The method comprises the following steps of: carrying out PCR (polymerase chain reaction) detection on the genes rol B and rol C of sterilized Brassica oleracea L.var.italic Planch. hairy roots; selecting the hairy root containing the genes rol B and rol C as a raw material; inoculating the raw material into an MS liquid culture medium containing methionine; carrying out constant-temperature shake cultivation for 14-16 d; then, addinga melatonin solution; after cultivation for 18 d, detecting the biomass and the proliferation multiple of the hairy root; and detecting the content of glucoraphanin and sulforaphane in the hairy root, as well as a glucoraphanin and sulforaphane releasing amount in the culture medium. By use of the method, the multiplication capacity of the hairy root and the synthetic ability of a secondary metabolism substance are improved, in addition, through the control of physical factors, the releasing of secondary metabolites, i.e., the glucoraphanin and the sulforaphane, in the hairy root to the culture medium as well as a conversion rate between the glucoraphanin and the sulforaphane can be improved, and a feedback inhibition effect caused by the accumulation of the glucoraphanin and the sulforaphane can be lowered.

Description

technical field [0001] The invention relates to a broccoli hairy root culture system, in particular to a method for synthesizing and releasing glucoraphanin and sulforaphane in the broccoli hairy root. Background technique [0002] Sulforaphane in broccoli is one of the single active ingredients with the strongest and best effect among the natural anti-cancer substances discovered so far, and it has unique anti-cancer and anti-cancer effects. Myrosinase and glucoraphanin are located in separate cells. When plants are subjected to exogenous mechanical damage, diseases and insect pests, myrosinase reacts with glucoraphanin under specific conditions (pH 5~8) to generate sulforaphane. Therefore, a large amount of glucoraphanin synthesis and the promotion of glucoraphanin to sulforaphane conversion are beneficial to increase the production of sulforaphane. The accumulation of glucoraphanin and sulforaphane tends to lead to the occurrence of feedback inhibition effect, so releas...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A01H4/00
CPCA01H4/00A01H4/001
Inventor 马绍英李胜卢旭马蕾包金玉张秀民田鹏张晓玲杨洁
Owner GANSU AGRI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com