Mouse embryo test blastocyst cell staining and counting kit for assisted reproductive technology
An assisted reproductive technology and blastocyst cell technology, which is applied in the field of blastocyst cell staining and counting kits for mouse embryo test for assisted reproductive technology, and can solve the problems of inability to identify the effects of embryo development and the like
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Embodiment 1
[0068] The mouse embryo test blastocyst cell staining and counting kit for assisted reproductive technology of the present invention is used to detect the influence of several embryo culture fluids commonly used in clinical practice on the in vitro culture of embryos, and compare the quality of different culture fluids.
[0069] 1. The tested culture medium is the three commonly used embryo culture medium for assisted reproductive technology in the market: (1) M16 culture medium; (2) Vitrolife (www.vitrolife.com) culture medium, ( G-1 TM PLUS, G-2 TM PLUS) and (3) Cook (COOK, www.cookmedical.com) culture medium (K-SICM, K-SIBM).
[0070] 2. Detection process:
[0071] 1) Collection of fertilized eggs in mice: 20 hours after injection of hCG, female mice were killed by neck dislocation, and fertilized eggs were collected in the ampulla of oviduct. The collected flocculent fertilized egg masses were placed in hyaluronidase (150 μg / mL), and the cumulus and granulosa cells arou...
Embodiment 2
[0090] The embryo toxicity of the newly developed embryo transfer catheter for assisted reproductive technology is detected by using a mouse embryo test blastocyst cell staining and counting kit for assisted reproductive technology of the present invention, so as to determine whether it meets the quality requirements of consumables for assisted reproductive technology.
[0091] The test consumable is the embryo transfer catheter, which can consist of several different parts. The raw materials of these parts may also vary. The detection process is as follows:
[0092] 1. According to different parts, make the extraction liquid preparation.
[0093] According to the Chinese national standard (YYT1434-2016), under aseptic conditions, cut the catheter into sections with sterile instruments, cut them into pieces, put them in culture bottles and weigh them respectively; add M16 embryo culture medium at a ratio of 0.2 g / ml , on a shaker for 72 hours at 37°C in the dark, to leach po...
Embodiment 3
[0114] Use a mouse embryo test blastocyst cell dyeing and counting kit for assisted reproductive technology of the present invention to detect the influence of a certain specific embryo culture fluid composition on embryo development, to determine whether it meets the quality requirements of the culture fluid for assisted reproductive technology, Development of new embryo culture medium for assisted reproductive technology.
[0115] The components of embryo culture fluid detected are amino acids. Based on the traditional KSOM culture medium, compare the effects of adding all essential amino acids and non-essential amino acids to the KSOM culture medium on embryo development to determine whether amino acids can be used as a component of embryo culture medium to improve the embryo culture system of assisted reproductive technology . The detection process is as follows:
[0116] 1. The tested product is KSOM culture fluid plus essential and non-essential amino acids; the contro...
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