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Nano cerium peroxide catalyst and method for catalyzing TMB color development by using same

A technology of cerium peroxide and catalyst, applied in the field of analysis and detection, to achieve the effect of overcoming dependence, short cycle and mild reaction conditions

Inactive Publication Date: 2020-04-24
SHANDONG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] However, in the prior art, there is no report about cerium peroxide as a catalyst to catalyze the hydrogen peroxide generated by itself under acidic conditions by cerium ions to generate hydroxyl radicals.

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  • Nano cerium peroxide catalyst and method for catalyzing TMB color development by using same
  • Nano cerium peroxide catalyst and method for catalyzing TMB color development by using same
  • Nano cerium peroxide catalyst and method for catalyzing TMB color development by using same

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Embodiment 1

[0030] A kind of nano cerium peroxide catalyst, its preparation method comprises the steps:

[0031] Under the action of stirring, dissolve 1.012 g of cerium sulfate tetrahydrate and 5 g of polyvinylpyrrolidone in 250 mL of deionized water, then add 0.3 g of sodium hydroxide and 5 mL of 30% hydrogen peroxide in sequence, and react for 30 minutes to obtain the red Brown nanometer cerium peroxide catalyst.

[0032] The nano cerium peroxide catalyst obtained in this example is used to catalyze the color development of TMB, and the specific steps include: adding 1.6 mg nano cerium peroxide to 8 mL of phosphate buffer solution with different pH values ​​containing 0.125 mg / mL TMB (pH is respectively 7.4, 7.0, 6.4, 6.0, 5.4), form a mixed solution I, incubate at room temperature for 5 minutes to obtain a solution system II, and use a UV spectrophotometer (scanning at 652 nm) to observe the absorbance change of the obtained solution system II.

[0033] figure 1 It is the X-ray diff...

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Abstract

The invention discloses a nano cerium peroxide catalyst which is prepared from cerium sulfate tetrahydrate, polyvinylpyrrolidone, sodium hydroxide and hydrogen peroxide as raw materials at room temperature. The nano cerium peroxide catalyst can catalyze and oxidize 3, 3 ', 5, 5'-tetramethyl benzidine (TMB) under a certain pH condition to generate a colorless-to-blue color development reaction. Thenano cerium peroxide catalyst disclosed by the invention has the advantages of pH response color development, simple preparation process, short period, low synthesis cost and the like, and has important application prospects in the fields of biology, medicine, detection and the like.

Description

technical field [0001] The invention belongs to the technical field of analysis and detection, and in particular relates to a method for catalyzing TMB color development with a nanometer cerium peroxide catalyst. Background technique [0002] Chemodynamic therapy is an emerging tumor treatment technology, which uses nanoparticles to catalyze hydrogen peroxide in tumor cells to generate highly active and highly cytotoxic hydroxyl radicals (•OH) to kill tumor cells, which can overcome tumor Drug resistance in hypoxic environments. For example, nano-ferric oxide, nano-manganese oxide, etc. are often used as catalysts for chemodynamic therapy. However, endogenous hydrogen peroxide in cells will be gradually decomposed by catalase in cells, resulting in a decrease in concentration, which is not enough to be catalyzed by nanoparticles to generate sufficient hydroxyl radicals, which limits the development of chemodynamic therapy. [0003] Metal peroxides refer to compounds contai...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78B01J23/10C01B15/047
CPCB01J23/10C01B15/047G01N21/78
Inventor 李晓伟周浩周雨锋温广武
Owner SHANDONG UNIV OF TECH
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