Antibacterial biofilm composite microneedle and application thereof

A biofilm and anti-bacterial technology, applied in microneedles, needles, drug delivery, etc., can solve the problems of difficult to completely remove bacterial biofilm and wound healing, achieve good broad-spectrum and biocompatibility, and improve performance , the effect of a wide range of application prospects

Inactive Publication Date: 2020-05-01
GUANGDONG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is to overcome the defects and deficiencies that the bacterial biofilm is difficult to completely remove and thus cause the wound to heal, and to provide an anti-bacterial biofilm composite microneedle and its application

Method used

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  • Antibacterial biofilm composite microneedle and application thereof
  • Antibacterial biofilm composite microneedle and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Embodiment 1 antibacterial biofilm composite microneedle

[0037] A kind of antibacterial biofilm composite microneedle, its preparation method comprises the following steps:

[0038] S1. Dissolve chitosan and zinc nitrate with a molecular weight of 50KDa in water (the quality of chitosan, the quality of zinc nitrate and the volume ratio of water are 0.3:2:50), vacuum defoaming, to obtain chitosan zinc nitrate compound solution;

[0039] S2. the chitosan zinc nitrate composite solution that step S1 obtains is 0.2mL / cm 2 Spread on the PDMS microneedle mold, evacuate to 0.06MPa, hold the pressure for 2 minutes, deflate, repeat 3 times, dry, and peel off to obtain the antibacterial biofilm composite microneedle; wherein, the microneedle body The length of the microneedle is 340 μm, the diameter of the top of the microneedle body is 250 μm, and the diameter of the bottom of the microneedle body is 25 μm.

[0040] The schematic diagram of the antibacterial biofilm composi...

Embodiment 2

[0041] Example 2 Bacterial Biofilm Composite Microneedles

[0042] A kind of antibacterial biofilm composite microneedle, its preparation method comprises the following steps:

[0043] S1. Dissolve chitosan and zinc nitrate with a molecular weight of 10KDa in water (the quality of chitosan, the quality of zinc nitrate and the volume ratio of water are 0.1:1:50), vacuum defoaming, to obtain chitosan zinc nitrate compound solution;

[0044] S2. the chitosan zinc nitrate composite solution that step S1 obtains is 0.1mL / cm with consumption 2 Spread on the PDMS microneedle mold, evacuate to 0.04MPa, hold the pressure for 3min, deflate, repeat 4 times, dry, and peel off to obtain the antibacterial biofilm composite microneedle; wherein, the length of the microneedle The diameter of the top of the microneedle body is 100 μm, and the diameter of the bottom of the microneedle body is 10 μm.

Embodiment 3

[0045] Example 3 Bacterial Biofilm Composite Microneedles

[0046] A kind of antibacterial biofilm composite microneedle, its preparation method comprises the following steps:

[0047] S1. dissolving chitosan and zinc nitrate with a molecular weight of 80KDa in water (the quality of chitosan, the quality of zinc nitrate and the volume ratio of water are 0.1:1:10), vacuum defoaming, to obtain chitosan zinc nitrate compound solution;

[0048] S2. the chitosan zinc nitrate composite solution that step S1 obtains is 0.4mL / cm 2 Spread on the PDMS microneedle mold, evacuate to 0.08MPa, hold the pressure for 2.5min, deflate, repeat 3 times, dry, and peel off to obtain the antibacterial biofilm composite microneedle; wherein, the microneedle The length is 500 μm, the diameter of the top of the microneedle body is 300 μm, and the diameter of the bottom of the microneedle body is 50 μm.

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Abstract

The invention discloses an antibacterial biofilm composite microneedle and application thereof. The preparation method of the microneedle comprises the following steps: S1, dissolving chitosan and zinc nitrate in water, and performing defoaming in vacuum to obtain a chitosan-zinc nitrate composite solution; and S2, coating a PDMS microneedle mold with the chitosan-zinc nitrate composite solution obtained in the step S1, and carrying out filling, drying and stripping so as to obtain the antibacterial biofilm composite microneedle. The microneedle can pierce a bacterial biofilm and promote chitosan and Zn<2+> with antibacterial performance to be conveyed into the bacterial biofilm, the performance of the chitosan and Zn<2+> antibacterial biofilm is remarkably improved, the bacterial biofilmis effectively removed, and then rapid healing of chronic difficult-to-heal wounds is promoted. In addition, the preparation method of the microneedle is simple, low in cost, rich in raw material source and high in safety. Therefore, the microneedle has a wide application prospect in removing a bacterial biofilm or preparing a bacterial biofilm scavenger and preparing a chronic difficult-to-heal wound repair material.

Description

technical field [0001] The invention belongs to the technical field of micro-nano manufacturing. More specifically, it relates to an antibacterial biofilm composite microneedle and its application. Background technique [0002] Chronic wounds mainly include diabetic foot ulcers, pressure sores, venous ulcers of lower extremities and non-healing surgical site infections, which cannot be cured within a predetermined period of time, not only causing great pain to patients, but also facing the risk of amputation. Bacterial biofilm is a membrane structure formed by bacteria and their products, necrotic tissue, extracellular matrix, etc. embedded in the wound. Once formed, bacterial biofilm will greatly hinder wound healing, and has been identified as the main cause of chronic wounds. It is a difficult problem in clinical treatment. Therefore, timely destruction and elimination of bacterial biofilm on the wound surface has a positive effect on the healing of difficult-to-heal wo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61M37/00A61K9/00A61K47/36
CPCA61K9/0021A61K47/36A61M37/0015A61M2037/0046A61M2037/0053A61M2037/0061
Inventor 袁志山易新王成勇
Owner GUANGDONG UNIV OF TECH
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