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Method for promoting microorganisms to induce calcium carbonate precipitation by using algae-bacteria co-cultivation system

A technology of co-cultivation system and microorganisms, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of unseen technology research and application, and achieve the effect of increasing biomass

Pending Publication Date: 2020-05-01
NANCHANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the laboratory research is the pure culture of microorganisms to induce calcium carbonate precipitation. So far, there has been no technical research and application on the microbial induction of calcium carbonate through the artificial construction of microalgae and bacteria co-culture system.

Method used

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  • Method for promoting microorganisms to induce calcium carbonate precipitation by using algae-bacteria co-cultivation system
  • Method for promoting microorganisms to induce calcium carbonate precipitation by using algae-bacteria co-cultivation system
  • Method for promoting microorganisms to induce calcium carbonate precipitation by using algae-bacteria co-cultivation system

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Effects of co-cultivation of Chlorella and Bacillus pasteuriani on the growth of Chlorella

[0031] (1) Preparation of Bacillus pasteurii bacteria liquid: Bacillus pasteurii ATCC 6453 ( Sporosarcina Pasteurii (purchased from the American Microorganism Collection Center) was activated according to the conventional activation method, and the specific operation was as follows: inoculate Bacillus pasteurianus in NH 4 -YE solid culture and placed in a constant temperature incubator at 30°C for static culture for 24 to 72 hours, on a sterile workbench, use an inoculation loop to pick an appropriate amount of colonies and inoculate them into 10 mL of NH 4 -YE liquid medium for the first expansion culture, inoculate 100mL NH 4-YE liquid medium for the second expansion culture, so co-expansion culture 3 times. The culture condition is 30° C. in a constant temperature shaking incubator, the rotation speed is 120 rpm, and the culture time is 24 to 48 hours. The growth curve ...

Embodiment 2

[0038] Induced calcium carbonate precipitation process using algae-bacteria co-culture system

[0039] (1) if image 3 As shown in the flow chart, after chlorella and Bacillus pasteurian are activated and amplified, a cell suspension of chlorella and Bacillus pasteurian is prepared, and the volume ratio of the algae and the bacteria suspension is 3:2 (v / v) Inoculate into 100mL sterile modified BBM medium, culture for 3-5 days, until the chlorophyll concentration of Chlorella in the algae-bacteria co-cultivation system is 16-20mg / L.

[0040] (2) Preparation of calcification solution: prepare by weighing 11.1g of anhydrous calcium chloride and 6g of urea in 1L of water to prepare Ca 2+ Calcification solution with a concentration of 4g / L. The solution was sterilized by suction filtration.

[0041] (3) Induction process such as Figure 4 As shown, in the aseptic workbench, pipette 50 mL of the algae solution prepared in step (1) and the calcification solution in step (2) respe...

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Abstract

The present invention discloses a method for promoting microorganisms to induce calcium carbonate precipitation by using an algae-bacteria co-cultivation system. The method comprises the following steps: microalgae and urease bacteria are co-cultivated under a condition of adding a certain concentration of glucose, and growth of the microalgae is reflected by measuring chlorophyll in the co-cultivation system; and the algae-bacteria co-cultivation system is mixed with a calcification solution after shake cultivation to achieve a purpose of algae-bacteria co-inducing of calcium carbonate precipitation. By artificially constructing the microalgae and urease bacteria co-cultivation system, the method can increase biomass of the microalgae, can also promote the microorganisms to induce the calcium carbonate precipitation, and provide a novel and unique inducing precipitation method for microorganism mineralization.

Description

technical field [0001] The invention discloses a method for promoting microorganism-induced calcium carbonate precipitation through an algae-bacteria co-cultivation system, and specifically belongs to the technical field of microorganisms and microorganism-induced calcite precipitation. Background technique [0002] It is well known that microalgae and bacteria coexist in ecosystems for a long time, and there are many complex relationships between symbiosis and parasitism. There have been studies (Zhou Wenli, et al. Differential analysis of the susceptibility of different marine bait microalgae to antibiotics [J]. Journal of Wuhan University (Natural Science Edition), 2007(02):128-133.) It has been shown that some bacteria can detoxify water bodies The organic matter is decomposed into small molecules of organic acids, amino acids and ammonia, which provide nutrition for single-celled algae and promote the growth of algae. And many microalgae are co-cultivated with bacteria...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12N1/20C12P3/00C12R1/89C12R1/01
CPCC12N1/12C12N1/20C12P3/00
Inventor 周文广徐品品陈甫风钟凤陈思华戈成祥李晶晶
Owner NANCHANG UNIV
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