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A kind of Chrysobacterium aureus ddw4-2 strain and its application in degrading tetracycline antibiotics

A technology of tetracyclines and Chryseobacterium, applied in the direction of microorganism-based methods, bacteria, microorganisms, etc., can solve the problem of few types and achieve good degradation effect

Active Publication Date: 2021-07-02
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the types of tetracycline antibiotic-degrading microorganisms reported so far are still small, and new strain resources are urgently needed. Therefore, mining more tetracycline antibiotic-degrading bacteria has important application value

Method used

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  • A kind of Chrysobacterium aureus ddw4-2 strain and its application in degrading tetracycline antibiotics
  • A kind of Chrysobacterium aureus ddw4-2 strain and its application in degrading tetracycline antibiotics
  • A kind of Chrysobacterium aureus ddw4-2 strain and its application in degrading tetracycline antibiotics

Examples

Experimental program
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Effect test

Embodiment 1

[0025] Isolation, purification and identification of embodiment 1 Chryseobacterium (C.jejuense) DDW4-2 bacterial strain

[0026] (1) Separation and purification: In July 2017, a sewage sample was collected from a pig farm in Fujian, and 0.1 mL of fresh sample was added to 1 mL of 0.9% normal saline, and vortexed to mix. Pipette 20uL of the suspension into 4mL LB broth and incubate at 30°C and 180rpm for 12-16 hours. Subsequently, the above-mentioned culture solution was divided into LB agar medium containing 64ug / mL tetracycline, and cultured at 30° C. for 16-24 hours. Pick the purified single colony, use the same LB agar medium for further purification, and culture at 30°C for 16-24 hours to obtain a purified single colony.

[0027] (2) Identification of 16S rDNA strains: Scrape a ring of the above-mentioned purified single colony, and extract the genome of the strain according to the instructions of the Tiangen Bacteria Genomic DNA Extraction Kit (Tiangen Biochemical Techno...

Embodiment 2

[0029] Colony morphological characteristics and physiological and biochemical characteristics of embodiment 2 Chryseobacterium aureus (C.jejuense) DDW4-2 bacterial strain

[0030] Chryseobacterium (C.jejuense) DDW4-2 strain belongs to Chryseobacterium (Chryseobacterium), Gram-negative, short bacillus, no flagella, no spores. Obligate aerobics, the optimum growth temperature is 35°C, the nutrient requirements are not high, and it can grow on ordinary medium, but it grows weakly or does not grow at all on MacConkey medium. After 24 hours of cultivation, the bacteria formed smooth, neat, moist, yellow or orange colonies on the blood plate without hemolysis. Add a drop of 3% KOH solution to the colony formed by Chryseobacterium, and the yellow orange will turn red.

Embodiment 3

[0031] Example 3 Preservation of Chryseobacterium (C.jejuense) DDW4-2

[0032] The preservation information of Chryseobacterium (C.jejuense) DDW4-2 is as follows:

[0033] Preservation time: November 22, 2019;

[0034] Name of depository unit: Guangdong Microbial Culture Collection Center (GDMCC);

[0035] Deposit number: GDMCC No: 60821;

[0036] Address of Preservation Unit: 5th Floor, Building 59, Compound, No. 100 Xianlie Middle Road, Guangzhou;

[0037] Classification name: Chryseobacterium jejuense, abbreviated as C.jejuense.

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Abstract

The invention belongs to the technical field of biodegradation of antibiotics, and relates to a degrading microorganism, in particular to a strain of Bacillus aureus DDW4-2 and its application in degrading tetracycline antibiotics. In order to discover more tetracycline antibiotic degrading bacteria, the invention provides A kind of Chryseobacterium jejuense DDW4-2 bacterial strain, this bacterial strain was preserved in Guangdong Province Microorganism Culture Collection Center on November 22, 2019, and the preservation number is GDMCC No: 60821, the 16S rDNA of this bacterial strain has such as SEQ ID NO : The nucleotide sequence shown in 3 has a good degrading effect on tetracycline antibiotics, and can degrade 48.4% of tetracycline after 16 hours, which provides a safe and environmentally friendly solution to the residual problem of tetracycline drugs.

Description

technical field [0001] The invention belongs to the technical field of biodegradation of antibiotics, and relates to a degrading microorganism, in particular to a strain of Chryseobacterium aureus DDW4-2 and its application in degrading tetracycline antibiotics. Background technique [0002] Tetracycline antibiotics are a class of broad-spectrum antibiotics. Their structures all contain naphthacene basic skeletons. The main types include tetracycline, oxytetracycline, chlortetracycline, doxycycline, minocycline, tigecycline and Iravacycline and others. Tetracycline antibiotics have been widely used all over the world due to their low cost, convenient use, and wide antibacterial spectrum. They are widely used in the prevention and treatment of livestock and poultry diseases and the promotion of livestock and poultry growth. At present, tetracycline antibiotics are one of the most widely used antibiotics in my country. According to statistics, the amount used in 2013 has reac...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20B09C1/10C02F3/34C12R1/01C02F101/38
CPCB09C1/10C02F3/34C02F2101/38C12N1/20C12N1/205C12R2001/01
Inventor 孙坚吴小亭沈祥广崔超月陈冲廖晓萍刘雅红
Owner SOUTH CHINA AGRI UNIV
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