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Application of shell powder of mytilus coruscus in aspect of bacteriostasis of marine bacteria

A technology of thick-shelled mussels and marine bacteria, which is applied in the field of antibacterial properties, can solve the problem of few types of shells, and achieve a stable antibacterial effect

Inactive Publication Date: 2020-05-08
SHANGHAI OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Seashells have a wide range of sources and great potential for development, but there are few shell species involved in antibacterial research

Method used

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  • Application of shell powder of mytilus coruscus in aspect of bacteriostasis of marine bacteria
  • Application of shell powder of mytilus coruscus in aspect of bacteriostasis of marine bacteria
  • Application of shell powder of mytilus coruscus in aspect of bacteriostasis of marine bacteria

Examples

Experimental program
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Effect test

Embodiment 1

[0032] In this example, Shewanella loihica, Vibrio splendidus and Pseudoalteromonasatlantica were inoculated in LB liquid medium for activation, and cultured on a shaker at 25° C. for 12 hours for later use. Add the shell powder of the calcined thick-shelled mussel into the LB liquid medium, and the concentration of the shell powder is 1mg / mL, 2mg / mL, 4mg / mL, 6mg / mL, 8mg / mL and 10mg / mL; The medium was placed on a magnetic stirrer and operated at 1000 rpm for 2 hours at 25° C.; the pH value of the liquid medium was measured and sterilized at 121° C. for use.

[0033] According to V 菌液 :V 培养基 = The ratio of 1:100 was added to the liquid culture medium. Control group 1 was pure LB liquid medium, control group 2 was LB liquid medium with different concentrations of shell powder added, experimental group 1 was pure LB liquid medium inoculated with experimental bacteria, and experimental group 2 was added different concentrations of shell powder and LB liquid medium of experiment...

Embodiment 2

[0036] The antibacterial time change experiment of the mussel calcined shell powder of this embodiment is as follows. Shewanellaloihica and Vibrio splendidus were inoculated in LB liquid medium for activation, and cultured on a shaker at 25°C for 12 hours for later use. Add the shell powder of the calcined thick-shelled mussel to the LB liquid medium, and the concentration of the shell powder is 10mg / mL; put the medium on a magnetic stirrer, run it at 1000rpm, 25°C for 2h; measure the liquid The pH value of the culture medium was sterilized at 121°C for use.

[0037] According to V 菌液 :V 培养基= The ratio of 1:100 was added to the liquid culture medium. Control group 1 was pure LB liquid medium, control group 2 was LB liquid medium with shell powder concentration of 10 mg / mL, experimental group 1 was pure LB liquid medium inoculated with experimental bacteria, and experimental group 2 was added shell powder with a concentration of 10mg / mL and LB liquid medium of experimental ...

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Abstract

The invention provides an application of shell powder of mytilus coruscus in the aspect of bacteriostasis of marine bacteria, which comprises the following steps: cleaning, drying, grinding and calcining waste shells of mytilus coruscus to obtain the shell powder; inoculating marine bacteria to be detected into the liquid culture medium for activation, then adding the shell powder into the liquidculture medium for reaction, and sterilizing the reaction product for later use In the invention, the shell powder disclosed by the invention has different antibacterial effects on shewanella, vibriosplendidus and pseudoalteromonas at different concentrations, and the 24-hour antibacterial rates respectively reach 93.6%, 92.1% and 95.7%; the antibacterial effects of the calcined shell powder on shewanella bacteria and vibrio splendidus tend to be stable within 3 hours and 6 hours respectively, and the antibacterial rates of the shell powder are not remarkably different along with the increaseof time; therefore, the shell powder of the mytilus coruscus has antibacterial activity on certain marine bacteria, the antibacterial effect can tend to be stable in a short time, and a new directionis provided for resource utilization of shells of waste mytilus coruscus.

Description

technical field [0001] The invention belongs to the technical field of antibacterial materials, and in particular relates to the application of mussel shell powder in the antibacterial properties of marine bacteria. Background technique [0002] Bacteriostasis is the process of inhibiting the activity of microorganisms in the system to be treated, reducing their reproductive ability or resisting reproduction. In the marine environment, some marine bacteria are pathogenic bacteria of marine organisms; some bacteria will participate in the process of corrosion, denaturation, pollution and destruction of various marine materials; under certain conditions, the accumulation of marine bacterial metabolites will poison the culture. The environment, such as the hazards of ammonia and hydrogen sulfide to biological farming. As the environmental damage to marine bacteria has been paid more and more attention, many researchers have paid attention to non-toxic, efficient and safe biolo...

Claims

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Application Information

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IPC IPC(8): A01N63/10A01P1/00
Inventor 梁萧孙悦彭莉华冯丹丹杨金龙
Owner SHANGHAI OCEAN UNIV
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