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Method for preserving and activating ganoderma spores

A technology of ganoderma spores and ganoderma fruiting bodies, applied in the field of preservation and activation of ganoderma spores, can solve the problems of low germination rate and difficult preservation of ganoderma spores, and achieve the effects of increasing germination rate, preventing dryness due to water shortage, and high germination rate

Pending Publication Date: 2020-05-08
GUANGXI XIANCAOTANG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a method for preserving and activating Ganoderma lucidum spores in view of the low germination rate of Ganoderma lucidum spores and difficulty in preservation

Method used

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Examples

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Effect test

Embodiment 1

[0030] A method for preserving and activating ganoderma spores, including the following steps:

[0031] a. Take the spores just collected from the Ganoderma lucidum fruiting body and immediately soak them in liquid nitrogen for 30 seconds, then take them out, and soak them in a deactivation solution at -20°C for 220 seconds to get deactivated spores;

[0032] Among them, the deactivation solution includes the following raw materials in parts by weight: 25 parts of Anabaena, 27 parts of Phaeodactylum tricornutum, 5 parts of hijiki, 8 parts of kelp, 0.13 parts of cellulase, 0.06 parts of protease, sodium alginate lysis 0.03 parts of enzymes, 26 parts of fresh aloe vera, and 0.75 parts of iris.

[0033] The preparation method of deactivation solution is: take Anabaena, Phaeodactylum tricornutum, kelp, hijiki, and kelp, add cellulase and sodium alginate lyase to enzymatically hydrolyze for 200 minutes to obtain enzymatic hydrolysate, and take fresh Aloe, remove the leaf flesh, mash, add...

Embodiment 2

[0041] A method for preserving and activating ganoderma spores, including the following steps:

[0042] a. Take the spores just collected from the fruit body of Ganoderma lucidum, immediately soak them in liquid nitrogen for 10 seconds, then take them out, and soak them in a deactivation solution at -40°C for 120 seconds to get deactivated spores;

[0043] Among them, the deactivation solution includes the following raw materials in parts by weight: 15 parts of Anabaena, 13 parts of Phaeodactylum tricornutum, 1 part of hijiki, 4 parts of kelp, 0.07 parts of cellulase, 0.02 parts of protease, sodium alginate lysis 0.01 part of enzyme, 16 part of fresh aloe vera, 0.15 part of iris.

[0044] The preparation method of the deactivation solution is: take Anabaena, Phaeodactylum tricornutum, kelp, hijiki, and kelp, add cellulase and sodium alginate lyase to enzymatically hydrolyze for 100 minutes to obtain the enzymatic hydrolysate, and take fresh Aloe vera, remove the leaf flesh, mash, ad...

Embodiment 3

[0052] A method for preserving and activating ganoderma spores, including the following steps:

[0053] a. Take the spores just collected from the Ganoderma lucidum fruiting body and immediately soak them in liquid nitrogen for 15 seconds, then take them out, and soak them in a deactivation solution at -35°C for 145 seconds to get deactivated spores;

[0054] Among them, the deactivation liquid includes the following raw materials in parts by weight: 17 parts of Anabaena, 16.5 parts of Phaeodactylum tricornutum, 2 parts of hijiki, 5 parts of kelp, 0.09 parts of cellulase, 0.03 parts of protease, sodium alginate lysis 0.02 parts of enzymes, 18 parts of fresh aloe vera, 0.3 parts of iris.

[0055] The preparation method of the deactivation solution is: take Anabaena, Phaeodactylum tricornutum, kelp, hijiki, and kelp, add cellulase and sodium alginate lyase to enzymatically hydrolyze for 125 minutes to obtain the enzymatic hydrolysate, and take fresh Aloe vera, remove the leaf flesh, m...

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Abstract

The invention provides a method for preserving and activating ganoderma spores and particularly relates to the technical field of edible mushroom breeding. According to the method, ganoderma spores which are just collected are subjected to liquid nitrogen freezing and deactivating by adopting a deactivating solution, are refrigerated in improved liquid paraffin and can be stored for 3-5 years; andthe ganoderma spores are activated by adopting microwave and activating liquid, and are resuscitated to achieve a high germination rate. The method is simple, is easy to operate and controllable in process, and is applicable to spore storage and germination promotion in ganoderma spore breeding studies.

Description

【Technical Field】 [0001] The invention relates to the technical field of edible fungus breeding, in particular to a method for preservation and activation of Ganoderma lucidum spores. 【Background technique】 [0002] Ganoderma has always been regarded as an elite of plants and trees in China. It has the reputation of "Seocho" and "Xiancao". In Japan, it is called Wannian velvet and is a precious medicinal (edible) fungus. "Shen Nong's Materia Medica" pointed out: Chi Zhi (i.e. Ganoderma lucidum) is "bitter, calm, and non-toxic", and is mainly used to "knot in the chest, benefit the heart, nourish the middle, increase wisdom, and not forget", "long eating, light body, immortality, and prolong life." . "Compendium of Materia Medica" records that Ganoderma lucidum has magical effects such as "nourishing and strengthening, prolonging life, strengthening joints, and curing deafness", and it is listed as the top-grade medicine. [0003] It is precisely because of the magical effect of G...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/04C12N1/38C12N13/00
CPCC12N1/04C12N1/38C12N13/00
Inventor 刘长斯龙丹萍石卢宁黄宗延
Owner GUANGXI XIANCAOTANG PHARMA
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