A three-channel electrochemiluminescent sensor and its application in cell detection
A technology of luminescence detection and electrochemistry, which is applied in the direction of chemiluminescence/bioluminescence, analysis of materials through chemical reactions, and electrochemical variables of materials, etc. and other problems, to achieve the effect of improving detection throughput, rapid detection, and simple design
Active Publication Date: 2022-01-07
NANJING UNIV
View PDF0 Cites 0 Cited by
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
For example, on the one hand, because cancer cells have multiple characteristic markers, and most current reports are based on the detection of a single marker, the reliability of cancer cell diagnosis is not high; on the other hand, using a single ECL probe labeled on the cell surface However, due to changes in certain analytical environments and light-sensitive efficiency of ECL instruments, the absolute value of a single ECL signal will also change, which may cause certain measurement errors, especially in low-level analysis
Therefore, it is difficult to establish a cell analysis method with stable ECL signal and reliable detection results
[0003] Due to the poor conductivity of the cells and the small space between the cells and the electrodes, the cells have serious steric hindrance on the electrodes, causing the ECL probes below the cells to not fully respond, and it is difficult to provide a reference signal that is not affected by the cells. Limits the application of ratiometric ECL technology in cell detection
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View moreImage
Smart Image Click on the blue labels to locate them in the text.
Smart ImageViewing Examples
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0039] 1) Drug and instrument information:
[0040] Luminol, aniline, phytic acid, tripropylamine (TPA), 3-aminopropyltriethoxysilane (APTES), potassium persulfate (K 2 S 2 o 8 ), hyaluronic acid (HA), tris(2,2'-bipyridyl) ruthenium(II) trichloride hexahydrate (Ru(bpy) 3 Cl 2 ·6H 2 O), folic acid (FA), ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC), and hydroxysuccinimide (NHS) were purchased from Sigma-Aldrich (USA). Hydrogen tetrachloroaurate trihydrate (HAuCl 4 ·3H 2 O) Synthesized by Shanghai Yuanye Biotechnology Co., Ltd. Melamine, ammonium persulfate ((NH 4 ) 2 S 2 o 8 ), triton X-100, tetraethylorthosilicate (TEOS), ammonia solution (NH 3 ·H 2 (25%) and 1-hexanol were obtained from Aladdin Industrial Co., Ltd. (Shanghai, China). Epidermal growth factor (EGF) was purchased from Cell Signaling Technology, USA. All solutions used deionized water.
[0041] MPI-E detection system (Xi'an Ruimai) was used for electrochemiluminescence analysis. Scanning Ele...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More PUM
Login to View More Abstract
The invention belongs to the field of biotechnology, and particularly relates to a three-channel electrochemiluminescence sensor and its application in cell detection. The sensor includes a polyaniline-based working electrode, an Ag / AgCl electrode and an electrochemiluminescence analyzer; Among them, three working electrodes and three Ag / AgCl electrodes were respectively inserted into three independent electrolytic cells, and connected with the electrochemiluminescence analyzer in parallel; among them, one working electrode was modified with luminol-functionalized gold Nanoparticles, providing a reference signal, while performing Ru(bpy) on the cells on the other two working electrodes 3 2+ and g‑C 3 N 4 Labeling, providing two detection signals; finally, the cells are measured according to the ratio between the detection signal and the reference signal; the present invention obtains three independent electrochemiluminescent signals during one potential cycle scanning process, and simultaneously provides two A method for detecting target cells. The electrochemiluminescent three-channel design of the invention is simple, improves the detection flux, is easy to operate, and has strong practicability.
Description
technical field [0001] The invention belongs to the field of biotechnology, in particular to a three-channel electrochemiluminescent sensor and its application in cell detection. Background technique [0002] Electrochemiluminescence (ECL) combines the advantages of electrochemiluminescence and chemiluminescence. Due to its low background interference, simple equipment, and good spatiotemporal controllability, ECL has become a powerful analytical method in the fields of drug analysis, immunoassay, environmental analysis, and clinical diagnosis. However, when using ECL technology to detect cancer cells, there are often certain limitations. For example, on the one hand, because cancer cells have multiple characteristic markers, and most current reports are based on the detection of a single marker, the reliability of cancer cell diagnosis is not high; on the other hand, using a single ECL probe labeled on the cell surface However, due to changes in certain analytical environ...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More Application Information
Patent Timeline
Login to View More Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/76G01N27/48
CPCG01N21/76G01N27/48
Inventor 朱俊杰刘根陈子轩马诚
Owner NANJING UNIV