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Oridonin callus culture medium with increased content of oridonin and rosmarinic acid

A technology of oridonin A and rosmarinic acid is applied in the fields of botanical equipment and methods, gardening, gardening tools/equipment, etc., which can solve the problems of unexplored culture process and the accumulation of metabolites, etc., and achieve shortened induction time. The effect of generating cycle time, increasing yield and reducing losses

Active Publication Date: 2021-05-04
PROYA COSMETICS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, in "Study on Suspension Culture Technology of Rubescens japonicus Cells", through the research of various key factors affecting the suspension culture system of Rubescens japonicus cells, the suspension culture system of Rubescens japonicus cells was established, but the culture process and metabolites were not discussed. Correlation between accumulation; "Effects of Nitrogen and Carbon Sources on Callus Growth and Accumulation of Rosmarinic Acid in Rubescens japonicus", by changing the ratio of nitrogen and carbon sources in the medium to increase the growth of rosemary acid production, but the study did not effectively solve the problem of browning, which will seriously affect the survival rate of callus

Method used

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  • Oridonin callus culture medium with increased content of oridonin and rosmarinic acid
  • Oridonin callus culture medium with increased content of oridonin and rosmarinic acid
  • Oridonin callus culture medium with increased content of oridonin and rosmarinic acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031]A concrete formulation of Winter Lingchen Callus, the specific formulation method is as follows: 3% MS medium (composition is shown in Table 1), 2.5% 1,5-dehydrated-D-glucohyl alcohol, 2.5% KNO3(NH)4)2SO4Mixtures, 0.1% Plant polyamine, 2% bitter elements, 0.002% lauroyl arginine ethyl hydrochloride, 0.4% agar, margin deionized water. Kno3(NH)4)2SO4The molar ratio is 1.8: 1.

Embodiment 2

[0033]An inducing medium induced by Winter Linggrass, the specific formulation method is as follows: including 4% MS medium (consisting of Table 1), 2% 1,5-dehydrated-D-glucool, 3% KNO3(NH)4)2SO4Mixtures, 2% plant polyamine, 5% bitter elements, 0.004% lauroyl arginine ethyl hydrochloride, 0.35% agar, marga deionized water. Kno3(NH)4)2SO4The molar ratio is 3: 1.

Embodiment 3

[0035]The induction medium of Winter Linggrass, the specific formulation method is as follows: including 5% MS medium (consisting of Table 1), 2.5% 1,5-dehydrated-D-glucohydrol, 2% KNO3(NH)4)2SO4Mixtures, 0.6% plant polyamine, 3% bitter elements, 0.003% lauroyl arginine ethyl hydrochloride, 0.35% agar, margin deionized water. Kno3(NH)4)2SO4The molar ratio is 2: 1.

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Abstract

The invention relates to an oridonium callus induction culture medium for increasing the content of oridonin A and rosmarinic acid, which is characterized in that it is composed of components with the following mass ratio: 3%~5% MS medium, 2%~ 2.5% 1,5‑Anhydro‑D‑Glucitol, 2~3% KNO 3 with (NH 4 ) 2 SO 4 0.1%~2% plant polyamine, 2%~5% matrine, 0.002%~0.004% lauroyl arginine ethyl ester hydrochloride, 0.3%~0.5% agar, the balance is deionized water . The invention has the beneficial effect of simultaneously increasing the contents of oridonin and rosmarinic acid in the callus tissue of the oridonium grass.

Description

Technical field[0001]The present invention belongs to the field of plant cell culture, and in particular, the present invention relates to Winter Lining Callus Cancer medium capable of improving Winter Lingli and aqueous acid content.Background technique[0002]Winter Ling is also known as ice grass, taking grass, for lip-shaped fragrant tea dishes, is widely growing in central Henan, Shanxi, Shaanxi and other central regions, suitable for the growth environment of subtropical and temperate monsoon climate, plants It is rich in winter and rosemary, both are antioxidant, anti-inflammatory and antibacterial efficacy. Winter Lingnicin is a seashell-type four-ring diterpenoid natural organic compound, which is mainly used in clinical anti-tumor therapy drugs, and the efficacy is obvious; the rosemary acid is a polyphenol compound, which is widely used in the field of cosmetics. By removing free radicals to antioxidant effects, the lipid peroxidation can inhibit, and has a broad-spectrum a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001
Inventor 崔丽涛黄洁芳陈强李芡芡徐晓明王丽娜毕永贤蒋丽刚
Owner PROYA COSMETICS
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