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Lactobacillus parafarraginis GBW-HB1903 and application thereof

A technology of Lactobacillus pastauropa, cgmccno.18391, applied in the field of microorganisms, can solve problems such as difficulties, and achieve the effects of ensuring stable operation, promoting and total nitrogen removal efficiency, and broad application prospects

Active Publication Date: 2020-05-12
QINGDAO SHANGDE BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are not many microorganisms that can significantly and efficiently use nitrate nitrogen in the system. It is difficult to quickly remove the total nitrogen in wastewater and make it up to the standard in a short period of time. However, it can be used efficiently by adding it to the wastewater system. Microbial agents that degrade nitrate nitrogen have become an important method and measure to solve this problem

Method used

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  • Lactobacillus parafarraginis GBW-HB1903 and application thereof
  • Lactobacillus parafarraginis GBW-HB1903 and application thereof
  • Lactobacillus parafarraginis GBW-HB1903 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1: Screening, isolation and identification of Lactobacillus pasteri GBW-HB1903

[0034] 1. Screening and purification of strains of Lactobacillus pasteri GBW-HB1903

[0035]Take 2 mL each of municipal sewage, industrial sewage and landfill leachate water samples, add them to 50 mL of PBS buffer solution, shake for 5 minutes to fully mix the samples, centrifuge at 1000 rpm for 5 minutes, collect the sample supernatant, and set aside. Take 10mL of the supernatant of the above samples and add them to conical flasks filled with 200mL nitrate nitrogen degrading bacteria enrichment and screening culture medium, culture at 30°C, 150rpm constant temperature shaker for 2 days, and enrich for 3 times. After gradient dilution of the cultured bacterial suspension, 100 μL was spread on the solid medium for isolation and purification of nitrate nitrogen degrading bacteria, and cultured in a 30°C incubator. After 48 hours, single colonies of different shapes were picked and s...

Embodiment 2

[0040] Example 2: Growth characteristics and physiological and biochemical characteristics of Lactobacillus pasteri GBW-HB1903

[0041] 1. Growth determination of Lactobacillus pasteri GBW-HB1903

[0042] Inoculate the psychrophilic Bacillus GBW-HB1903 cultured on the slant into the improved NB medium, and culture it on a constant temperature shaker at pH 6.5-7.5 and 25-35°C for 24 hours to prepare GBW-HB1903 bacterial liquid, and sample it every 2 hours. The absorbance value was measured at OD600nm, and the growth curve was drawn. Such as figure 2 As shown, the experimental results show that GBW-HB1903 is in the growth retardation phase in the first 4 hours of culture, and then enters the logarithmic growth phase. After 16-18 hours of culture, it reaches the end of logarithmic growth, and the number of bacteria reaches 5.0×10 9 cfu / mL, 18-22h is the stable growth period, and then enters the decline period, thus completing the entire growth cycle.

[0043] 2. Physiological...

Embodiment 3

[0048] Embodiment 3: Growth comparison test of bacterial strain GBW-HB1903 under different oxygen content conditions

[0049] In order to test the survival performance of Lactobacillus pasteri GBW-HB1903 under different oxygen content conditions, its growth under different oxygen content was detected. Inoculate equal amounts of Lactobacillus pasterneri GBW-HB1903 into the NB liquid medium under different oxygen content conditions, control its pH to 6.5-7.5, and obtain Lactobacillus pasterneri GBW-HB1903 bacterial liquid after culturing for 20 hours, and observe its growth in different Growth in media under conditions of oxygen content. The results are shown in Table 2, Lactobacillus pasteri GBW-HB1903 can grow normally in the range of dissolved oxygen less than 0.5mg / L or greater than 1.0mg / L, the most suitable dissolved oxygen is 2-3mg / L, and Strain GBW-HB1903 belongs to facultative anaerobic bacteria.

[0050] Table 2 Lactobacillus pasteri GBW-HB1903 growth in different ox...

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Abstract

The invention provides a lactobacillus parafarraginis GBW-HB1903 and an application thereof. The classification name of the lactobacillus parafarraginis GBW-HB1903 is lactobacillus parafarraginis, andthe preservation number is CGMCC No.18391. The bacterial colony of the lactobacillus parafarraginis GBW-HB1903 is circular and milky white, has a diameter of 1-2 microns, has a smooth, moist and glossy surface, has a slightly convex middle part, is opaque, has a uniform edge and is free of halo. The lactobacillus parafarraginis GBW-HB1903 is facultative anaerobe, and can grow under a condition that the dissolved oxygen content is less than 0.5mg / L or more than 1.0mg / L, and the lactobacillus palmati GBW-HB1903 has capability of efficiently degrading nitrate nitrogen in wastewater under an aerobic or anoxic condition, can be applied to removing total nitrogen in wastewater, promotes and improves removal efficiency of total nitrogen in a sewage treatment system, and has a wide application prospect.

Description

technical field [0001] The invention belongs to the technical field of microbes, and relates to a strain of Lactobacillus pasteri GBW-HB1903 and its application. Background technique [0002] With the continuous improvement of social economy and living standards, the output of domestic and industrial wastewater is increasing, so the amount of sewage treatment in various regions is also continuously increasing, which brings great pressure to sewage treatment units. A large amount of sewage discharge will also seriously affect and destroy the health of the ecological environment, causing eutrophication of a large number of rivers, lakes and coastal waters, causing a large number of algae outbreaks, and damaging the health and ecological balance of animals and plants in the water. In addition to the large amount of COD, nitrogen is also one of the main culprits in the sewage that causes the deterioration of the water body. The nitrogen-containing organic matter in the sewage t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C02F101/16C12R1/225
CPCC12N1/20C02F3/34C02F2101/16C12R2001/225C12N1/205
Inventor 王熙涛袁绍辉赵金超
Owner QINGDAO SHANGDE BIOTECH