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Bifidobacteria lysate and preparation method thereof
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A technology of bifidobacteria and cell lysis, applied in the field of cell lysates, to achieve the effects of maintaining integrity, increasing yield, and improving fermentation efficiency
Active Publication Date: 2020-05-12
GUANGZHOU RIDGEPOLE BIOLOGICAL TECH CO LTD
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Currently, there is little research on how to strengthen epidermal cells
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preparation example Construction
[0025] In order to solve the above problems, the present invention provides a preparation method of bifidobacteria lysate, comprising the following steps: fermenting, centrifuging and crushing bifidobacteria in a culture solution containing millet extract.
[0026] Preferably, its preparation method comprises the following steps:
[0027] (1) Preparation of culture solution: take a certain weight of millet, add 12-18 times deionized water by weight, then heat and reflux for extraction for 1.5-2.5 hours, and cool to 55-75°C to obtain an extract; then in the extract Add enzymatic hydrolysis for 10-14h, centrifuge to obtain supernatant; take supernatant and add MRS medium at 40-60g / L, mix well and sterilize to obtain culture medium;
[0028] (2) Strain activation: Bifidobacteria were activated on the MRS slant medium, cultured at 35-39°C for 45-50 hours, and then the slant-activated strains were added to the MRS liquid test tube at an added amount of 0.5-1.5% for culture. In the...
Embodiment 1
[0063] A preparation method of bifidobacteria lysate, comprising the following steps:
[0064] (1) Preparation of culture solution: take a certain weight of millet, add 15 times deionized water by weight, then heat and reflux for extraction for 2 hours, and cool to 65-70°C to obtain an extract; then add enzymatic hydrolysis to the extract for 12h , centrifuge at 6000r / min for 15min to obtain supernatant; take supernatant and add MRS medium at 54g / L, mix well and sterilize to obtain culture medium, sterilization conditions are 121 ℃, 15min;
[0065] The enzymes are high temperature resistant α-amylase and composite protease; the weight percentage of high temperature resistant α-amylase in the extract is 0.2%, and the weight percentage of composite protease in the extract is 0.1%.
[0066] (2) Strain activation: Bifidobacterium bifidum was inoculated on MRS slant medium for activation, cultured at 37°C for 48 hours, and then the slant-activated strain was added to MRS liquid tes...
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Abstract
The application belongs to the technical field of lysates and skin care, and particularly relates to a bifidobacteria lysate and a preparation method thereof. The preparation method of the bifidobacteria lysate provided by the invention comprises the following steps of performing fermentation on bifidobacteria in culture fluid containing millet extracting solutions, performing centrifuging and performing crushing, so that the bifidobacteria lysate is obtained. The bifidobacteria lysate disclosed by the invention can effectively promote expression of intermediate filament relevant proteins between keratinocytes, including filaggrin FLG, loricrin LOR, involucrin IVL, keratin KRT-10 and para-transglutaminase TGM-1, further contributes to transformation of the keratinocytes to cuticle, and isquite important in the respect of maintaining integrity of skin barriers.
Description
technical field [0001] The application belongs to the technical field of lysates, and in particular relates to a bifidobacteria lysate and a preparation method thereof. Background technique [0002] Sensitive skin is referred to as "sensitive skin", also known as intolerant skin, reactive skin, hyper-reactive skin. Sensitive skin is not a disease but a state. Teacher Wang Xuemin defined sensitive skin in "New Progress in Dermatology and Venereology 2004" as a type of skin that is more reactive than normal skin and has the characteristics of violent reaction to the weak influence of external factors. It has high sensitivity , poor tolerance, easy reactivity characteristics. Factors such as the deterioration of the living environment, the increase of work pressure and the improper use of cosmetics have gradually increased the proportion of people with sensitive skin. According to statistics, sensitive skin has become the fastest-rising skin problem among consumers. According...
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