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Cryptomeria fortunei CfCCR gene and application thereof

A cedar and gene technology, applied in the field of plant molecular biology and genetic engineering, can solve the problems of lower lignin content, loose secondary wall structure, abnormal plant development, etc., to increase wood density, improve quality and quality, The effect of improving plant stress resistance

Active Publication Date: 2020-05-12
NANJING FORESTRY UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Previous studies have found that: the faster the inhibition of CCR activity decreases, the more the corresponding lignin content decreases, which affects the growth and development of plants
Inhibiting CCR activity will reduce lignin content, reduce secondary wall deposition, and relax secondary wall structure. In severe cases, it will cause abnormal plant development

Method used

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  • Cryptomeria fortunei CfCCR gene and application thereof
  • Cryptomeria fortunei CfCCR gene and application thereof
  • Cryptomeria fortunei CfCCR gene and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] 1. Cloning and expression analysis of Cryptomeria CfCCR gene

[0029] 1. Cloning of Cryptomeria CfCCR gene

[0030] Select Cedar cedar growing well in the Arboretum of Nanjing Forestry University, remove the epidermis, scrape the vascular tissue with a blade, extract total RNA and reverse transcribe it into cDNA. According to the conserved sequence analysis data of the CCR gene of Chinese fir and the differentially expressed genes analyzed by the high-throughput sequencing data of the Cedar transcriptome in our laboratory, a CCR homologous sequence was screened out using the NCBI online tool Blast, and primers were designed.

[0031] Design specific amplification primers CCR-F and CCR-R, carry out PCR amplification with cDNA as template, the recovery of PCR product, connect pBI121 carrier ( figure 1 ).

[0032] The specific amplification primers CCR-F and CCR-R are:

[0033] CCR-F: 5′-TAATTGTTGTAAATACGCTTG-3′

[0034] CCR-R: 5′-AATTACAGTTCTTATTGGCAT-3′

[0035] The...

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Abstract

The invention discloses a cryptomeria fortunei CfCCR gene and an application thereof, belonging to the technical fields of plant molecular biology and gene engineering. The cryptomeria fortunei ligninsynthesis regulation CfCCR gene provided by the invention has a nucleotide sequence as shown in SEQ ID NO. 1, and the amino acid sequence of an expression protein of the cryptomeria fortunei lignin synthesis regulation CfCCR gene is as shown in SEQ ID NO. 2. The CfCCR gene is a key rate-limiting enzyme of a lignin synthesis specific pathway, regulates the expression of related genes for synthesizing lignin in plants through encoding protein, and plays an important role in biosynthesis and stress resistance of lignin. The tobacco transformed with the CfCCR gene is increased in lignin expression and developed in cell walls. Thus, the thickness of xylem cell walls in plant stems like cryptomeria fortunei can be changed by expressing the CfCCR gene, so the wood density is increased, and the wood quality and plant stress resistance are improved.

Description

technical field [0001] The invention belongs to the technical fields of plant molecular biology and genetic engineering, and specifically relates to a cedar CfCCR gene and its application Background technique [0002] Cryptomeria fortunei (Cryptomeria fortunei) belongs to the Taxodiaceae (Taxodiaceae) Cryptomeria (CryptomeriaD.Don) plant, also known as the long-leaved peacock pine, tree, conical crown; bark reddish brown, fibrous, large branches in whorls, twigs thin Long, often drooping, with subulate leaves slightly curved inwards, curved inwards at the apex, and stomatal lines on the four sides. It is a unique tree species in my country and an important timber species. Its wood has a wide range of value and can also be used as ornamental plants and medicinals. The degree of lignification of cedar will affect the density of cedar wood, thus affecting the quality of cedar. [0003] CCR plays a key role in the specific pathway of lignin synthesis. It is the first key enzyme ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/53C12N9/02C12N15/84C12N1/21A01H5/00A01H6/82
CPCC12N9/0008C12N15/8255C12Y102/01044
Inventor 徐进华慧胡海亮郭臻昊杨俊杰
Owner NANJING FORESTRY UNIV
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