Cryptomeria fortunei CfCCR gene and application thereof
A cedar and gene technology, applied in the field of plant molecular biology and genetic engineering, can solve the problems of lower lignin content, loose secondary wall structure, abnormal plant development, etc., to increase wood density, improve quality and quality, The effect of improving plant stress resistance
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[0028] 1. Cloning and expression analysis of Cryptomeria CfCCR gene
[0029] 1. Cloning of Cryptomeria CfCCR gene
[0030] Select Cedar cedar growing well in the Arboretum of Nanjing Forestry University, remove the epidermis, scrape the vascular tissue with a blade, extract total RNA and reverse transcribe it into cDNA. According to the conserved sequence analysis data of the CCR gene of Chinese fir and the differentially expressed genes analyzed by the high-throughput sequencing data of the Cedar transcriptome in our laboratory, a CCR homologous sequence was screened out using the NCBI online tool Blast, and primers were designed.
[0031] Design specific amplification primers CCR-F and CCR-R, carry out PCR amplification with cDNA as template, the recovery of PCR product, connect pBI121 carrier ( figure 1 ).
[0032] The specific amplification primers CCR-F and CCR-R are:
[0033] CCR-F: 5′-TAATTGTTGTAAATACGCTTG-3′
[0034] CCR-R: 5′-AATTACAGTTCTTATTGGCAT-3′
[0035] The...
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