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Method for increasing yield of chitin deacetylase through mixed bacterium fermentation

A technology of deacetylase and mixed bacteria fermentation, applied in the biological field, can solve the problems of unrealized industrialization, long fermentation time, and less bacteria, and achieve the effect of wide industrial application prospects and rapid accumulation

Active Publication Date: 2020-05-15
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are only a small amount of literature reports on chitin deacetylase-producing bacteria at home and abroad, and chitin deacetylase from microbial sources is mainly fungi, and bacteria are less
Moreover, due to a series of problems such as long fermentation time and low enzyme yield in the production of chitin deacetylase by microbial fermentation, the enzyme has not been industrialized.

Method used

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  • Method for increasing yield of chitin deacetylase through mixed bacterium fermentation
  • Method for increasing yield of chitin deacetylase through mixed bacterium fermentation
  • Method for increasing yield of chitin deacetylase through mixed bacterium fermentation

Examples

Experimental program
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Effect test

Embodiment 1

[0019] Embodiment 1 A kind of mixed-bacteria fermentation produces the method for chitin deacetylase

[0020] (1) Seed culture (enzyme-producing bacteria and associated bacteria are cultured separately under the same seed culture conditions):

[0021] Culture conditions: stirring speed is 200rpm, temperature is 37°C, fermentation is 24h;

[0022] Seed medium: peptone 10g / L, yeast extract powder 5g / L, sodium chloride 10g / L, pH7.0;

[0023] (2) Fermentation culture

[0024] Mix the cultured enzyme-producing bacteria and associated bacteria seed liquids according to the mixing ratio of 1:1 and then inoculate and ferment; the inoculum amount is 10%, the stirring speed is 200rpm, the temperature is 37°C, and the fermentation is 48h. The fermentation liquid is regularly taken for chitin Deacetylase enzyme activity detection, and at the same time make separate strains (Rhodococcus equi CGMCC No.14861, Staphylococcus epidermidis ATCC12228) fermentation enzyme production control, the...

Embodiment 2

[0038] Embodiment 2 A method for producing chitin deacetylase by mixed bacteria fermentation

[0039] (1) Seed culture (enzyme-producing bacteria and associated bacteria are cultured separately under the same seed culture conditions):

[0040] Culture conditions: stirring speed is 180rpm, temperature is 34°C, fermentation is 24h;

[0041] Seed medium: peptone 8g / L, yeast extract powder 5g / L, sodium chloride 8g / L, pH6.5;

[0042] (2) Fermentation culture

[0043] Mix the cultured enzyme-producing bacteria and associated bacteria seed solution according to the mixing ratio of 1:0.5, 1:1.5, 1:2.0 and 1:4, and then inoculate and ferment; the inoculation amount is 10%, the stirring speed is 180rpm, and the temperature is 37 ℃, fermented for 48 hours, and the fermentation broth was taken regularly for detection of chitin deacetylase enzyme activity, and at the same time, the enzyme production by fermentation of a single strain was used as a control;

[0044] Fermentation medium: ...

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Abstract

The invention belongs to the technical field of biologics and in particular relates to a method for increasing the yield of chitin deacetylase through mixed bacterium fermentation. The method comprises the following steps: adding staphylococcus epidermidis ATCC12228 (an accompanying strain) in the fermentation process of rhodococcus equi CGMCC No.14861 (an enzyme generation strain), and performingmixed bacterium fermentation, wherein the enzyme activity of each mL of a fermentation broth is up to 2996.2U. The enzyme yield of fermentation is increased by 20.2 times when being compared with that of single bacterium fermentation of enzyme generation strains, and compared with the prior art, the mixed bacterium fermentation process provided by the invention is capable of rapidly accumulatingchitin deacetylase, achieves a high-yield effect, and has wide industrial application prospects.

Description

Technical field: [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for increasing the yield of chitin deacetylase by mixed bacteria fermentation. Background technique: [0002] Chitin, also known as chitin, scientifically named (1,4)-2-acetylamino-2-deoxy-β-D-glucan, is a natural biological content second only to cellulose Amino polysaccharides mainly exist in invertebrates such as shrimp, insects, seaweed, fungi and yeast. However, chitin is insoluble in water, acid, alkali and organic solvents, so it has little commercial value, and the product chitosan after deacetylation is widely used in medicine, food, chemical industry, cosmetics and other industries. There are many problems in the chemical method currently used in the production of chitosan, such as long reaction time, high energy consumption, unstable product quality, and especially the discharge will cause huge environmental pollution. [0003] Chitin deacetylase ca...

Claims

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Application Information

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IPC IPC(8): C12N9/80C12N1/20C12R1/01C12R1/45
CPCC12N9/80C12Y305/01041C12N1/20
Inventor 马钦元王敏申雁冰屠琳娜张兴郭亚波
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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