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Nanogold colorimetric method based on anti-agglomeration and determination of silver ions

A nano-gold colorimetric method and silver ion technology, applied in the field of analysis and detection, can solve the problems of high background signal, insufficient precision, and low accuracy, and achieve the effects of broad application prospects, good selectivity, and high sensitivity

Active Publication Date: 2020-05-15
信阳学院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This operation has the characteristics of long time-consuming, high cost, high background signal, etc., and there are many factors affecting the aggregation of nano-gold in the solution, resulting in insufficient precision and accuracy of this method.

Method used

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  • Nanogold colorimetric method based on anti-agglomeration and determination of silver ions
  • Nanogold colorimetric method based on anti-agglomeration and determination of silver ions
  • Nanogold colorimetric method based on anti-agglomeration and determination of silver ions

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Embodiment 1

[0027] Embodiment 1 The specific operation of a kind of nano-gold colorimetric method based on the anti-agglomeration principle of the present invention and the determination of silver ions

[0028] (1) Preparation of calibration curve:

[0029] a. Use deionized water to prepare silver ion solutions with concentrations of 1 nM, 3 nM, 5 nM, 7 nM, and 9 nM for use; prepare 2 μM methimazole solution with deionized water for use; prepare pH 7.5 BR buffer solution for use;

[0030] b. Take 200 μL of nano-gold solution, add 200 μL of 2 μM methimazole solution, then add 400 μL of BR buffer solution with pH 7.5, shake well and incubate for 5 minutes, scan the UV-visible spectrum, and measure the concentration of the solution at 520 nm and 660 nm. The absorbance at nm, the ratio of the absorbance of the solution A 520 / A 660 as A 0 ;

[0031] c. Take 200 μL 2 μM methimazole, 200 μL silver ion standard solution of different concentrations, add 200 μL BR buffer solution, shake w...

Embodiment 2

[0037] Embodiment 2 Feasibility analysis of the present invention

[0038] In order to verify that the present invention is based on the anti-agglomeration principle of nano-gold colorimetric determination of silver ions, the applicant has carried out the following tests.

[0039] Take 200 μL nano-gold and add 600 μL BR buffer solution as solution I; take 200 μL nano-gold and add 200 μL 1 nM silver ion solution, then add 400 μL BR buffer solution as solution II, incubate for 5 minutes; take 200 μL nano-gold Solution, add 200 μL 2 μM methimazole and 400 μL BR buffer solution as solution III, incubate for 5 minutes; take 200 μL 2 μM methimazole, add 200 μL 1 nM silver ion solution and 200 μL BR buffer solution for incubation After 10 minutes, add 200 μL nano-gold as solution IV and incubate for 5 minutes; take solutions I, II, III, and IV for UV-visible spectrum scanning, and the scanning results are as follows: figure 2 ;Depend on figure 2 It can be seen that the ultraviole...

Embodiment 3

[0041] Embodiment 3 The selectivity analysis of the present invention

[0042] Ca was examined experimentally 2+ 、Cd 2+ 、Cu 2+ , Fe 2+ 、K + , Mg 2+ , Mn 2+ 、Al 3+ 、Ni 2+ , Pb 2+ , Zn 2+ , Hg 2+ 、Ag + Respectively to this method (the concrete operation of embodiment 1) i.e. the response of the system, thus investigate this method to Ag + selectivity. In the experiment, Ag + At a concentration of 5 nM, Pb 2+ 、Cu 2+ , Hg 2+ The concentration is 50 nM, and the concentration of other ions is 100 nM. The other experimental conditions are the same. The experimental results are as follows: Figure 5 As shown, compared to other metal ions A 520 / A 660 The ratio of Ag + It shows a significantly high absorbance ratio, indicating that the method has good selectivity to silver ions.

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Abstract

The invention provides a nanogold colorimetric method based on an anti-agglomeration principle and a method for determining the content of silver ions in a water sample by using the nanogold colorimetric method. The method comprises the following steps: taking 200 [mu] L of a nanogold solution prepared by reduction of sodium citrate, adding 200 [mu] L of a methimazole solution with a certain concentration, adding 400 [mu] L of a BR buffer solution, carrying out uniform shaking incubation for 5 min, carrying out ultraviolet-visible spectrum scanning, determining the absorbance of the solution at 520 nm and 660 nm, and taking the absorbance ratio A520 / A660 of the solution as A0; taking 200 [mu] L of methimazole with a certain concentration and 200 [mu] L of a silver ion solution with a certain concentration; adding 200 [mu] L BR buffer solution, shaking incubation for 10 minutes, adding 200 [mu] L of a nanogold solution; shaking and incubating for 5 minutes, carrying out ultraviolet-visible spectrum scanning, determining the absorbance of the solution at 520 nm and 660 nm, using the absorbance ratio A520 / A660 of the solution as A1, and the change of the absorbance ratio after the silver ions are added is in linear relation with the concentration of the silver ions so that the content of the silver ions in the water sample can be rapidly, sensitively and accurately measured. The method is good in selectivity, high in sensitivity and wide in detection range, only an ultraviolet spectrophotometer is needed for measurement without a large instrument, the operation requirement islow and the method has wide application prospects.

Description

technical field [0001] The invention relates to the field of analysis and detection, in particular to an anti-agglomeration-based nano-gold colorimetric method and a method for detecting silver ions in water samples. Background technique [0002] Silver ions are one of the trace elements in human tissues. They have a bactericidal effect and are widely used in cosmetics, building materials, medicine and other industries. Silver ion is also a kind of heavy metal ion. Silver ion in electroplating, electronics, photosensitive and other industries will cause great harm to the environment and human body if it enters the water. Currently, the World Health Organization (WHO) recommends that the maximum concentration of silver ions in drinking water be 0.1 mg / L (927 nM), and the United States Environmental Protection Agency recommends that the maximum concentration of silver ions in water is 0.05 mg / L (464 nM). Therefore, the detection of silver ions is very important. [0003] At ...

Claims

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Application Information

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IPC IPC(8): G01N21/78G01N21/31G01N1/34G01N1/44
CPCG01N21/78G01N21/3151G01N1/34G01N1/44G01N2021/3155
Inventor 毛丽恵王倩倩刘仁植杨潇剑王大祥雒宇豪
Owner 信阳学院
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