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Lyophilized powder with repairing activity as well as preparation method and application thereof

A freeze-dried powder and active technology, which is applied in the field of freeze-dried powder with repairing activity and its preparation, can solve the problems of loss of biological activity, existence of virus infection, etc., and achieves the effect of promoting regeneration, good biological activity, and repairing and healing gastric ulcer wounds. Effect

Pending Publication Date: 2020-05-19
广州市暨源生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Collagen is currently mainly extracted from animal connective tissue through acid and alkali hydrolysis. During the extraction process, it will inevitably lose part of its biological activity. It will cause heterogeneous rejection when it acts on the human body, and there is a hidden danger of virus infection.

Method used

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  • Lyophilized powder with repairing activity as well as preparation method and application thereof
  • Lyophilized powder with repairing activity as well as preparation method and application thereof
  • Lyophilized powder with repairing activity as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] A freeze-dried powder with repairing activity, the freeze-dried powder is compounded by A-phase components and B-phase components with a mass ratio of 1:1, wherein:

[0040] The Phase A component is made from the following raw materials:

[0041]

[0042]

[0043] The B-phase component is made from the following raw materials:

[0044] 10 parts deionized water

[0045] Blue Copper Peptide 0.03 parts

[0046]Recombinant human type Ⅰ collagen 0.2 parts

[0047] The preparation method of the above-mentioned freeze-dried powder with repairing activity comprises the following steps:

[0048] 1. Dissolve the components of Phase A evenly, sterilize at 121°C for 20 minutes, and leave to cool for later use;

[0049] 2. Dissolve the components of Phase B evenly, mix them with Phase A evenly, and put them into vials;

[0050] 3. Put the filled freeze-dried powder liquid into the freeze-dryer plate, and close the door;

[0051] 4. Pre-freezing stage: pre-freezing temper...

Embodiment 2

[0055] A freeze-dried powder with repairing activity, the freeze-dried powder is compounded by A-phase components and B-phase components with a mass ratio of 1:1, wherein:

[0056] The Phase A component is made from the following raw materials:

[0057]

[0058] The B-phase component is made from the following raw materials:

[0059] 10 parts deionized water

[0060] Blue Copper Peptide 0.03 parts

[0061] Recombinant human type Ⅰ collagen 0.5 parts

[0062] The preparation method of the above-mentioned freeze-dried powder with repairing activity comprises the following steps:

[0063] 1. Dissolve the components of Phase A evenly, sterilize at 121°C for 20 minutes, and leave to cool for later use;

[0064] 2. Dissolve the components of Phase B evenly, mix them with Phase A evenly, and put them into vials;

[0065] 3. Put the filled freeze-dried powder liquid into the freeze-dryer plate, and close the door;

[0066] 4. Pre-freezing stage: pre-freezing temperature -35 ℃...

Embodiment 3

[0070] A freeze-dried powder with repairing activity, the freeze-dried powder is compounded by A-phase components and B-phase components with a mass ratio of 1:1, wherein:

[0071] The Phase A component is made from the following raw materials:

[0072]

[0073] The B-phase component is made from the following raw materials:

[0074] 20 parts deionized water

[0075] Blue Copper Peptide 0.08 parts

[0076] Recombinant Human Type Ⅰ Collagen 0.8 parts

[0077] The preparation method of the above-mentioned freeze-dried powder with repairing activity comprises the following steps:

[0078] 1. Dissolve the components of Phase A evenly, sterilize at 121°C for 20 minutes, and leave to cool for later use;

[0079] 2. Dissolve the components of Phase B evenly, mix them with Phase A evenly, and put them into vials;

[0080] 3. Put the filled freeze-dried powder liquid into the freeze-dryer plate, and close the door;

[0081] 4. Pre-freezing stage: pre-freezing temperature -35 ℃...

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Abstract

The invention belongs to the field of cosmetics, and discloses lyophilized powder with repairing activity as well as a preparation method and application thereof. The lyophilized powder is composed ofan A phase component and a B phase component (the mass ratio is 1:1); the A phase component is prepared from the following raw materials in parts by mass: 70-80 parts of deionized water, 3-15 parts of mannitol, 1-4 parts of spirulina maxima, 0.1-0.6 part of disodium hydrogen phosphate, and 0.1-0.4 part of sodium dihydrogen phosphate; and the B phase component is prepared from the following raw materials in parts by mass: 5-15 parts of deionized water, 0.001-0.3 part of glycyl-L-histidyl-L-lysine-copper, and 0.01-1 part of recombinant human type I collagen.

Description

technical field [0001] The invention belongs to the field of cosmetics, in particular to a freeze-dried powder with repairing activity and its preparation method and application. Background technique [0002] Freeze-drying technology, also known as vacuum freeze-drying technology, is to freeze the product below the eutectic point temperature, fully freeze the water, and then use the sublimation principle to remove the water in the product at an appropriate temperature and vacuum degree to obtain a dry product. Technology. Compared with drying methods such as sun-drying and spray-drying, freeze-drying technology has many advantages, which makes its development in food, ceramics, archaeology and other industries change with each passing day. Especially in the preservation of biological products, freeze-drying technology has played an indispensable role. [0003] The instability of biomolecules such as protein, DNA, RNA, and metabolites originates from hydrolysis reactions in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/99A61K8/65A61K8/64A61Q19/00
CPCA61K8/99A61K8/65A61K8/64A61Q19/00A61K2800/84A61K2800/5922
Inventor 黄亚东谢伟权黄志朱德勇肖巧学
Owner 广州市暨源生物科技有限公司
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