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N-terminal peptide of brown planthopper vitellogenin with ovarian targeting function and use thereof

A vitellogenin, brown planthopper technology, applied in the direction of DNA/RNA fragments, peptides, peptide sources, etc., can solve the problems of large molecular weight, non-transportable carrier development, difficult fusion expression and purification of target proteins, etc., and achieves the effect of broad application prospects.

Active Publication Date: 2021-12-31
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The molecular weight of insect Vg protein is usually relatively large (>150kDa), and it is difficult to express and purify the fusion with the target protein, so it cannot be directly used in the development of transport vectors

Method used

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  • N-terminal peptide of brown planthopper vitellogenin with ovarian targeting function and use thereof
  • N-terminal peptide of brown planthopper vitellogenin with ovarian targeting function and use thereof
  • N-terminal peptide of brown planthopper vitellogenin with ovarian targeting function and use thereof

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Embodiment Construction

[0032] The specific implementation manners of the present invention will be further described in detail below in conjunction with the accompanying drawings. It should be understood that these examples are used to illustrate the present invention and not to limit the scope of the present invention. The implementation conditions used in the examples can be further adjusted according to the specific application conditions, and the unspecified implementation conditions are usually the conditions in routine experiments. In the present invention, unless otherwise specified, the scientific and technical terms used herein have the meanings commonly understood by those skilled in the art. Moreover, the laboratory operation steps of cell culture, molecular genetics, nucleic acid chemistry, and immunology used herein are all routine steps widely used in the corresponding fields. Meanwhile, in order to better understand the present invention, definitions and explanations of relevant term...

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Abstract

The invention relates to high-efficiency biomolecule delivery technology, and aims to provide an N-terminal peptide of brown planthopper vitellogenin with ovarian targeting function and its application. The amino acid sequence of the peptide is shown in SEQ ID NO:1. The use of the peptide segment as a transport carrier for targeting biomolecules in the ovary of the brown planthopper is to use the N-terminal peptide segment of the vitellogenin of the brown planthopper to carry the biological molecule into the egg through the maternal hemolymph of the brown planthopper; the biological molecule is a nucleic acid, an oligopeptide , polypeptides, proteins, carbohydrates, lipids, nanoparticles, or small molecule compounds, or any complexes of them. The peptides provided by the present invention can be used as biomolecule transport carriers, especially as gene editing element transport carriers to carry biomolecules into the ovary of brown planthopper. Compared with other exogenous polymer materials or exogenous proteins, VgNPP is derived from the brown planthopper itself, so it has no toxic and side effects on the brown planthopper and has a very broad application prospect.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to an N-terminal peptide of vitellogenin from brown planthopper with ovarian targeting function and its use, and in particular to a technical method for high-efficiency delivery of biomolecules. Background technique [0002] Brown planthopper (Nilaparvata lugens (Stal)), Hemiptera, Planthopperidae, monophagous pest, directly sucks phloem sap of rice and spreads rice virus to harm rice. The outbreak of brown planthopper can lead to "lice burning" of large areas of rice. At present, brown planthopper has become an important pest in rice growth in many Asian countries. Due to its important economic value and ecological significance, for a long time, the main research on brown planthopper has focused on reproductive development, pesticide resistance, wing type differentiation, long-distance migration and so on. The RNA interference technology applied to the study of gene function plays an ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/435C07K19/00C12N15/12C12N15/62A61K47/42
CPCC07K14/43563A61K47/42C07K2319/00
Inventor 徐海君薛文华王琪琦
Owner ZHEJIANG UNIV