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Method for extracting melanin from palm meal

A technology for palm meal and melanin, applied in chemical instruments and methods, azo dyes, organic dyes, etc., can solve the problems of affecting the molecular structure of melanin, high cost, and large amount of chemical reagents, and achieve high enzymatic hydrolysis efficiency and mild action conditions. , which is conducive to the effect of subsequent extraction

Inactive Publication Date: 2020-05-19
WUHAN SUNHY BIOLOGICAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there are methods for extracting melanin from black beans, black sesame, tea leaves, ganoderma lucidum, ebony leaves, etc., most of which are conventional solvent methods. The disadvantage is that the amount of chemical reagents is large, resulting in high cost, and the extraction process will affect the molecular structure of melanin.

Method used

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  • Method for extracting melanin from palm meal

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Crush the palm meal into a powder with a particle size of 60 mesh and add it to soak in water to form a mixed solution. The mass ratio of the palm meal powder to water is 1:10; add β-mannanase that accounts for 4.0% of the mass of the palm meal powder in the compound enzyme preparation , xylanase accounting for 1.0% of the mass of palm meal powder, cellulase accounting for 4.0% of the mass of palm meal powder, pectinase accounting for 1.0% of the mass of palm meal powder, and α-amylase accounting for 1.0% of the mass of palm meal powder , lipase accounting for 3.0% of the mass of palm meal powder and protease accounting for 1.0% of the mass of palm meal powder, carrying out oscillating enzymolysis on the mixed solution at 35° C. for 8 hours, and filtering the mixed solution after oscillating enzymolysis to obtain filter residue and filtrate, After soaking the filter residue with a concentration of 20g / L sodium hydroxide solution, filter and collect the filtrate produced ...

Embodiment 2

[0030] Crush the palm meal into a powder with a particle size of 80 mesh and add it to soak in water to form a mixed solution. The mass ratio of the palm meal powder to water is 1:10; add β-mannanase that accounts for 3.0% of the mass of the palm meal powder in the compound enzyme preparation , Xylanase accounting for 0.5% of the mass of palm meal powder, cellulase accounting for 3.0% of the mass of palm meal powder, pectinase accounting for 0.5% of the mass of palm meal powder, and α-amylase accounting for 1.0% of the mass of palm meal powder , lipase accounting for 2.0% of the mass of palm meal powder and protease accounting for 1.0% of the mass of palm meal powder, carrying out oscillating enzymolysis on the mixed solution at 35° C. for 8 hours, and filtering the mixed solution after oscillating enzymolysis to obtain filter residue and filtrate, After soaking the filter residue with a concentration of 25g / L sodium hydroxide solution, filter and collect the filtrate produced ...

Embodiment 3

[0032] Crush the palm meal into a powder with a particle size of 80 mesh and add it to soak in water to form a mixed solution. The mass ratio of the palm meal powder to water is 1:10; add β-mannanase that accounts for 0.8% of the mass of the palm meal powder in the compound enzyme preparation , Xylanase accounting for 0.2% of the mass of palm meal powder, cellulase accounting for 1.0% of the mass of palm meal powder, pectinase accounting for 0.1% of the mass of palm meal powder, 0.5% of the mass of palm meal powder α-amylase 1. Lipase accounting for 1% of the mass of palm meal powder and protease accounting for 0.5% of the mass of palm meal powder, carrying out oscillating enzymolysis on the mixed solution at 35° C. for 9 hours, and filtering the mixed solution after oscillating enzymolysis to obtain filter residue and filtrate, After soaking the filter residue with a concentration of 15g / L sodium hydroxide solution, filter and collect the filtrate produced after soaking the fi...

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Abstract

The invention provides a method for extracting melanin from palm meal. The method comprises the following steps: weighing palm meal powder, adding the palm meal powder into water, and soaking to forma mixed solution; adding a compound enzyme preparation, and carrying out oscillation enzymolysis on the mixed solution at a first preset temperature; filtering the mixed solution subjected to oscillation enzymolysis to obtain filter residues and filtrate; and soaking the filter residues in an alkali liquor, filtering, collecting the filtrate generated after the filter residues soaked in the alkaliliquor are filtered, evaporating and concentrating the filtrate generated after the filter residues soaked in the alkali liquor at a second preset temperature to form a concentrated solution, addinga hydrochloric acid solution into the concentrated solution to separate out precipitates, filtering, and drying.

Description

【Technical field】 [0001] The invention relates to the technical field of melanin extraction, in particular to a method for extracting melanin from palm dregs. 【Background technique】 [0002] The structure of melanin is stable, easily soluble in alkaline solution and ethanol, insoluble in water and acidic solution; it has anti-oxidation, anti-radiation, anti-ultraviolet and immune regulation functions, and has strong adhesion. At present, there are methods to extract melanin from black beans, black sesame seeds, tea leaves, ganoderma lucidum, ebony leaves, etc., most of which are conventional solvent methods. The disadvantage is that the amount of chemical reagents is large, resulting in high cost, and the extraction process will affect the molecular structure of melanin. [0003] In view of this, it is necessary to provide a novel method for extracting melanin from palm dregs to overcome the above defects. 【Content of invention】 [0004] The purpose of the present inventi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09B61/00C09B67/54
CPCC09B61/00C09B67/0096
Inventor 刘营周明苏丹王冠张成杰周樱詹志春
Owner WUHAN SUNHY BIOLOGICAL
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