Unlock instant, AI-driven research and patent intelligence for your innovation.

A method that greatly accelerates the enzyme-catalyzed conversion of carbon dioxide to formic acid

A carbon dioxide and formic acid technology, applied in the field of enzyme catalysis reaction, can solve the problems of accelerated reusability, acceleration, poor reusability, etc., and achieve the effects of improving reusability, simple and easy operation, and simplified preparation method

Active Publication Date: 2021-11-23
BEIJING INSTITUTE OF TECHNOLOGYGY
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the PDA / PEI-SiO as the carrier 2 Both CA and CA can accelerate the enzymatic conversion of carbon dioxide into formic acid, and CA can also overcome the PDA / PEI-SiO 2 The disadvantage of poor reusability, the synergy between the two finally achieved unprecedented acceleration effect and excellent reusability

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A method that greatly accelerates the enzyme-catalyzed conversion of carbon dioxide to formic acid
  • A method that greatly accelerates the enzyme-catalyzed conversion of carbon dioxide to formic acid
  • A method that greatly accelerates the enzyme-catalyzed conversion of carbon dioxide to formic acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0072] A method that can greatly accelerate the enzyme-catalyzed conversion of carbon dioxide into formic acid, where the enzyme-catalyzed CO 2 In the reaction system converted to formic acid, add PDA / PEI-SiO 2 -CA, then into CO 2 The gas is maintained at a certain pressure and reacts in a constant temperature oscillator. The reaction system contains 1mg / mL FDH and 50mM NADH, and the reaction is carried out at a concentration of 0.05M phosphate buffer (pH6.0), at a temperature of 37°C, a pressure of 0.5MPa, and an oscillator speed of 170rpm; PDA / PEI-SiO 2 -The consumption of CA is 0.2g / mL, after reacting 10min, formic acid output is 0.235mM, and reaction rate is 1.41mM / h, is 47 times of control experiment system (see attached Figure 4 ).

[0073] The formic acid output of the control experimental system without any addition was 0.05mM after 10min of reaction; adding the same mass (0.2g / mL) of PDA / PEI-SiO 2 In the system, the formic acid output after 10min of reaction was ...

Embodiment 2

[0078] A method that can greatly accelerate the enzyme-catalyzed conversion of carbon dioxide into formic acid, where the enzyme-catalyzed CO 2 In the reaction system converted to formic acid, add PDA / PEI-SiO 2 -CA, then into CO 2 The gas is maintained at a certain pressure and reacts in a constant temperature oscillator. The reaction system contains 0.2mg / mL FDH and 100mM NADH, and the reaction is carried out in 0.1M phosphate buffer (pH6.5), the temperature is 30°C, the pressure is 2MPa, the oscillator speed is 120rpm, PDA / PEI-SiO 2 -The dosage of CA is 0.5g / mL, after 30 minutes of reaction, the output of formic acid is 0.142mM, and the reaction rate is 0.852mM / h, which is obtained by adding PDA / PEI-SiO 2 -30 times before CA. PDA / PEI-SiO 2 - After CA was centrifuged from the reaction solution, it was washed 2-3 times with 0.1M phosphate buffer (pH 6.5) and then used again. When it was used for the 10th time, the effect was 93% of that when it was used for the first time....

Embodiment 3

[0082] A method that can greatly accelerate the enzyme-catalyzed conversion of carbon dioxide into formic acid, where the enzyme-catalyzed CO 2 In the reaction system converted to formic acid, add PDA / PEI-SiO 2 -CA, then into CO 2 The gas is maintained at a certain pressure and reacts in a constant temperature oscillator. The reaction system contains 2mg / mL FDH and 10mM NADH, and the reaction is carried out in 0.2M phosphate buffer (pH6.8), the temperature is 40°C, the pressure is 1MPa, the oscillator speed is 150rpm, PDA / PEI-SiO 2 -The dosage of CA is 0.4g / mL, after 20min of reaction, the output of formic acid is 0.314mM, and the reaction rate is 0.942mM / h, which is obtained by adding PDA / PEI-SiO 2 -42 times before CA. PDA / PEI-SiO 2 - After CA was centrifuged from the reaction solution, it was washed with 0.2M phosphate buffer (pH6.8) for 2 to 3 times and then used again. When it was used for the 10th time, the effect was 96% of that when it was used for the first time. ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
sizeaaaaaaaaaa
Login to View More

Abstract

The invention relates to a method capable of greatly accelerating the conversion of carbon dioxide into formic acid by enzyme catalysis, belonging to the field of enzyme catalysis. The purpose of the present invention is to provide a method that can greatly accelerate the conversion of enzyme-catalyzed carbon dioxide into formic acid; the method adds PDA / PEI-SiO 2 ‑CA accelerators catalyze the conversion of carbon dioxide to formic acid. Due to the PDA / PEI‑SiO as carrier 2 Both can accelerate the enzyme-catalyzed conversion of carbon dioxide to formic acid, and CA can also overcome the PDA / PEI‑SiO 2 The disadvantage of poor reusability, the synergy between the two finally achieved unprecedented acceleration effect and excellent reusability.

Description

technical field [0001] The invention relates to a method capable of greatly accelerating the conversion of carbon dioxide into formic acid by enzyme catalysis, belonging to the field of enzyme catalysis. Background technique [0002] CO 2 It is one of the most important greenhouse gases, which contribute to the increase of global temperature due to its large emission. CO 2 Catalytic hydrogenation or reaction with other raw materials to synthesize high value-added chemicals is an important way to reuse greenhouse gases. Compared with the traditional catalytic reaction process usually carried out under high temperature and pressure, the enzyme catalytic method has the advantages of high efficiency, high selectivity, mild reaction conditions, and no pollution to the environment, which is in line with the development trend of today's society. [0003] with CO 2 As a raw material or one of the raw materials, it can synthesize formic acid, methanol, malic acid, isocitric acid,...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/40
CPCC12P7/40
Inventor 刘文芳王彩红翟婷婷
Owner BEIJING INSTITUTE OF TECHNOLOGYGY