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Method for establishing fingerprint spectrum of bactericidal itching-relieving lotion

A technology of sterilization and antipruritic, fingerprint map, applied in the field of establishment of fingerprint map of sterilization and antipruritic lotion, can solve the problems of complex composition of sterilization and itching lotion, incomplete quality control indicators, etc., achieve comprehensive monitoring of product quality, avoid single Sexual and one-sided, high-tech effects

Active Publication Date: 2020-05-19
贵州长生药业有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The ingredients of antibacterial and antipruritic lotion are relatively complex, and it is obviously not comprehensive to use the ingredients of Sophora flavescens as the quality control index, which has certain limitations

Method used

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  • Method for establishing fingerprint spectrum of bactericidal itching-relieving lotion
  • Method for establishing fingerprint spectrum of bactericidal itching-relieving lotion
  • Method for establishing fingerprint spectrum of bactericidal itching-relieving lotion

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Experimental program
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Effect test

Embodiment 1

[0067] A method for establishing the fingerprint of a bactericidal and antipruritic lotion, comprising the following steps:

[0068] S1. Preparation of mixed reference solution:

[0069] Take matrine, astilbin, phellodendron, berberine hydrochloride, osthole, and atractylodes reference substances in appropriate amounts, accurately weigh them, and add methanol to make the concentrations 248.3, 190.7, 154.1, 198.5, 251.4, and 183.6, respectively. μg / mL mixed reference solution.

[0070] S2. Preparation of bactericidal antipruritic lotion sample solution:

[0071] Accurately weigh the bactericidal antipruritic lotion, add anhydrous methanol, ultrasonically extract for 30 minutes, let cool, filter under reduced pressure, take the supernatant and centrifuge at 13000r / min for 10 minutes to obtain the test solution.

[0072] S4. Preparation of single-flavor contrast medicinal material solution:

[0073] Crush Phellodendron Phellodendron, Sophora Sophora, Cnidium Fructus, Smilax Sm...

experiment example

[0086] 1. Inspection of extraction conditions

[0087] 1.1 Investigation of wavelength

[0088] Under the same conditions, compare the chromatograms at different wavelengths, the results are shown in figure 1 .

[0089] Among them, 1: 220nm; 2: 240nm; 3: 260nm; 4: 285nm; 5: 290nm; 6: 300nm; 7: 320nm; 8: 340nm.

[0090] The results showed that considering the number of chromatographic peaks, peak intensity, baseline stability and separation effect, 285nm was determined as the final detection wavelength.

[0091] 1.2 Inspection of chromatographic column

[0092] Taking the same sample solution of antibacterial and antipruritic lotion, under the same chromatographic conditions, the separation effects of the following five different C18 chromatographic columns were investigated, including: Waters XBridge C18 (4.6×250mm, 5μm); Comatex C18-AA (250mm ×4.6mm, 5μm); ZORBAX Eclipse Plus C18 (4.6×250mm, 5μm); Zorbax SB-C18 (4.6×250mm, 5μm); Phenomenex luna C18 (4.6×250mm, 5μm); fig...

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Abstract

The invention provides a method for establishing a fingerprint spectrum of a bactericidal itching-relieving lotion. The method comprises the following steps: S1, preparing a mixed reference substancesolution, namely precisely weighing matrine, astilbin, phellodendrine, berberine hydrochloride, cnidium lactone and atractylodes lancea reference substances respectively, and adding methanol to prepare the mixed reference substance solution; S2, preparing a bactericidal itching-relieving lotion sample solution; S3, preparing a single reference medicinal material solution; and S4, determining. Thefingerprint spectrum calibrates 16 common peaks and determines the five medicinal materials belonging to a prescription, the established fingerprint spectrum has high technical content, and the singleness and one-sidedness of the existing quality control method are avoided. An effective quality control basis is provided for the production of the bactericidal itching-relieving lotion, and a foundation is laid for the research on the correlation between the pharmacodynamic material basis and the pharmacological action of the bactericidal itching-relieving lotion.

Description

technical field [0001] The invention relates to the field of medical detection, in particular to a method for establishing a fingerprint of a bactericidal and antipruritic lotion. Background technique [0002] Bactericidal and antipruritic lotion is exclusively produced by Guizhou Changsheng Pharmaceutical Co., Ltd. It is prepared from 6 traditional Chinese medicines of Cortex Phellodendri, Sophora Sophora, Cnidium, Smilax, Borneol, and Atractylodes. It has the effects of clearing away heat and detoxification, killing insects and relieving itching. Insect, fungal, non-specific vaginitis and genital pruritus caused by profuse leukorrhagia, vulvar itching embolism. [0003] Existing research such as "Study on the HPL Fingerprint of Bactericidal and Antipruritic Lotion" established a C-fingerprint technology for bactericidal and antipruritic lotion, but only for the two characteristic peaks of matrine and sophocarpine. According to the investigation, the characteristic peaks o...

Claims

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Application Information

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IPC IPC(8): G01N30/88G01N30/86G01N30/34
CPCG01N30/88G01N30/8686G01N30/8679G01N30/34G01N2030/8809
Inventor 王冬梅姜楠赵磊程世娟高燕
Owner 贵州长生药业有限责任公司
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