Bifidobacterium longum BL986, freeze-dried powder prepared from same and application of freeze-dried powder
A technology of Bifidobacterium longum and BL986, applied in the direction of bifidobacteria, bacteria used in food preparation, application, etc., can solve the problems of uneven distribution, difficult dissolution, etc., to reduce the difficulty of dispersion, good dispersion effect, and protect the activity. Effect
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Embodiment 1
[0039] (1) Preparation of medium: Dissolve 15g of peptone, 8g of yeast extract, 15g of glucose and 0.5mL of Tween 80 in 1000mL of distilled water, and sterilize at 125°C for 15 minutes;
[0040] (2) Activation of lactic acid bacteria: insert Bifidobacterium longum BL986 into the sterilized medium, so that the number of original bacteria is 1.0×10 5 cfu / ml; ferment at 44°C for 72 hours to make the number of bacteria >5.0×10 9 cfu / ml;
[0041] (3) Cell collection: collect the lactic acid bacteria fermentation liquid, centrifuge at 7500r / min, 4°C for 12 minutes, discard the supernatant to collect the cell sediment;
[0042] (4) Freeze-drying of bacteria slime: Add freeze-drying protective agent and water to the precipitate collected in step (3), 90% of bacteria slime, 2% of skim milk powder, 8% of water, spread the thickness of 3cm, at -85°C Freezing overnight, vacuum freeze-drying with a vacuum freeze dryer for 30 hours to obtain a freeze-dried powder semi-finished product, th...
Embodiment 2
[0045] (1) Preparation of medium: Dissolve 8 g of peptone, 3 g of yeast extract, 15 g of glucose and 2 mL of Tween 80 in 1000 mL of distilled water, and sterilize at 120°C for 15 minutes;
[0046] (2) Activation of lactic acid bacteria: insert Bifidobacterium longum BL986 into the sterilized medium, so that the number of original bacteria is 1.0×10 4 cfu / ml; ferment at 35°C for 24 hours to make the number of bacteria >6.0×10 9 cfu / ml;
[0047] (3) Bacteria collection: collect the lactic acid bacteria fermentation liquid, centrifuge at 7500r / min, 4°C for 4 minutes, discard the supernatant and collect the bacterium sediment;
[0048] (4) Freeze-drying of bacteria slime: add freeze-drying protective agent and water to the precipitate collected in step (3), 80% of bacteria slime, 1% of trehalose, 18% of water, the thickness of the plate is 1.0cm, -75°C Freeze overnight, and vacuum freeze-dry for 18 hours with a vacuum freeze dryer to obtain a semi-finished freeze-dried powder. T...
Embodiment 3
[0051] (1) Preparation of medium: dissolve 10 g of peptone, 5 g of yeast extract, 20 g of glucose and 1.5 mL of Tween 80 in 1000 mL of distilled water, and sterilize at 122°C for 20 minutes;
[0052] (2) Activation of lactic acid bacteria: insert Bifidobacterium longum BL986 into the sterilized medium, so that the number of original bacteria is 1.0×10 4 cfu / ml; ferment at 40°C for 60 hours to make the number of bacteria >3.0×10 9 cfu / ml;
[0053] (3) Bacteria collection: collect the lactic acid bacteria fermentation liquid, centrifuge at 6000r / min, 4°C for 8 minutes, discard the supernatant and collect the bacterium sediment;
[0054] (4) Freeze-drying of bacteria slime: add freeze-drying protective agent and water to the precipitate collected in step (3), 90% of bacteria slime, VC0.3%, 0.8% of soybean lecithin, 8.9% of water, and the thickness of the plate is 2.0% cm, frozen overnight at -80°C, vacuum freeze-dried for 24 hours with a vacuum freeze dryer to obtain a semi-fin...
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