Preparation method of mesenchymal stem cells for treating acute liver failure

A technology of acute liver failure and mesenchymal stem cells, which is applied in the field of mesenchymal stem cell preparation, can solve the problems of limited clinical application and uncontrollable concentration of active ingredients in serum of liver damage, and achieve reasonable technical solutions, good multi-directional differentiation potential, and strong The effect of homing ability

Inactive Publication Date: 2020-05-29
WUXI PEOPLES HOSPITAL
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Problems solved by technology

However, the safety issues of genetic engineering and the uncontrollable concentration of active ingredien

Method used

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  • Preparation method of mesenchymal stem cells for treating acute liver failure
  • Preparation method of mesenchymal stem cells for treating acute liver failure
  • Preparation method of mesenchymal stem cells for treating acute liver failure

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Experimental program
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Embodiment

[0054] 1. Take male adult SD rats, 6-8 weeks old, weighing 180-200g, specific-pathogen free (SPF) level, purchased from Changzhou Cavens Experimental Animal Co., Ltd. (permit number: SCXK (Su ) 2016-0010) ﹞, raised in the SPF-level environment of the Animal Experiment Center of Wuxi People's Hospital (experimental unit use license number: SYXK (Su) 2015-0004).

[0055] 2. Preparation of IL-1β pretreated mesenchymal stem cells:

[0056] S1: Rats were killed under anesthesia, rat femurs were aseptically isolated, the bone marrow cavity was washed with sterile PBS, the bone marrow was collected and dispersed, and inoculated in DMEM / F12 medium containing 10% fetal bovine serum (FBS) for culture. After 72 hours The medium was replaced to remove non-adherent cells, and then the medium was changed every 3 days to obtain bone marrow mesenchymal stem cells.

[0057] S2: Bone marrow mesenchymal stem cells were selected, cultured in medium, and subcultured when the cell confluence reach...

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Abstract

The present invention discloses a preparation method of mesenchymal stem cells for treating acute liver failure. The mesenchymal stem cells with higher purity are successfully separated and cultured by using a full bone marrow wall-pasting method, the separated and cultured mesenchymal stem cells are subjected to an IL-1 beta pretreatment, during treatment, the IL-1 beta pretreatment obviously enhances expression of CXCR4 in the mesenchymal stem cells, homing capability of the mesenchymal stem cells to damaged liver is further improved, improvement effects of the mesenchymal stem cells on survival rate, liver functions, hepatic necrosis and hepatic cell apoptosis of rats with acute liver failure are obviously improved, and reconstruction of the liver is also further promoted. The mesenchymal stem cells obtained by the preparation method have excellent curative effects during treatment of the acute liver failure. The preparation method is simple in operation steps and reasonable in technical scheme, the IL-1 beta pretreatment can effectively improve the curative effects on the treatment of the acute liver failure, and the preparation method provides a new direction and a theoreticalbasis for the curative effects of the acute liver failure, and has higher practicability.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for preparing mesenchymal stem cells for treating acute liver failure. Background technique [0002] Acute liver failure (ALF) is a clinical syndrome characterized by hepatic decompensation and coagulation dysfunction caused by massive liver cell necrosis or dysfunction in a short period of time. Although with the development of medical diagnosis and treatment technology, the survival rate of acute liver failure has been significantly improved, but its mortality rate is still as high as 40%-62.2%. [0003] Orthotopic liver transplantation is currently the most effective treatment for acute liver failure, but the lack of donors, immune rejection and other reasons seriously limit its wide application in clinical practice. Hepatocyte transplantation can rapidly synthesize, detoxify, and promote bilirubin excretion, which can partially solve the problem of liver donor shortage....

Claims

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Application Information

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IPC IPC(8): C12N5/0775A61K35/28A61P1/16
CPCC12N5/0663A61K35/28A61P1/16C12N2509/00C12N2501/2301
Inventor 张清淋占强聂贺安方梅
Owner WUXI PEOPLES HOSPITAL
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