Ink-jet printing glucose detection test paper immobilized by sol-gel

A sol-gel and detection test paper technology, applied in the field of medical detection, can solve the problems of insufficient sensitivity of indication, short shelf life, uneven color development, etc., and achieve the effects of increased sensitivity, easy operation and uniform color development

Inactive Publication Date: 2020-05-29
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Solve the problems of short shelf life, uneven color development, and insuffi

Method used

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  • Ink-jet printing glucose detection test paper immobilized by sol-gel

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A kind of preparation of glucose detection test paper, according to the blueprint designed in advance such as figure 1 As shown, the diameter of the indication area is 0.7 cm; the channel width is 0.8 cm, and the length is 0.5 cm; the diameter of the liquid area to be tested is 1.0 cm, after printing out the drawing with a paraffin inkjet printer. Place the test paper in an electric blast drying oven at 120 degrees Celsius and heat it for 10 minutes, then take it out for use. Add 100ul sol-gel system to a 1ml centrifuge tube, then add 50ul tetramethylbenzidine solution, then add 20ul 2g / L peroxidase in PBS, 4ul 1g / L glucose oxidase in PBS. Vibrate for 30s with an oscillator until the system is uniform. Add 1ul of the above-mentioned indicator dropwise to the indicator area of ​​the test paper, place it in the air for 30 minutes, vacuum pack it, and put it in a foil bag for storage away from light.

Embodiment 2

[0031] A preparation of glucose detection test paper, according to the previously designed drawings, the diameter of the indication area is 0.7 cm; the width of the channel is 0.5 cm, and the length is 0.5 cm; the diameter of the liquid area to be tested is 0.6 cm, and the drawing is printed with a paraffin inkjet printer after coming out. Place the test paper in an electric blast drying oven at 150 degrees Celsius and heat it for 5 minutes, then take it out for use. Add 200ul sol-gel system to a 1ml centrifuge tube, then add 30ul tetramethylbenzidine solution, then add 10ul 2g / L peroxidase in PBS, 4ul 1g / L glucose oxidase in PBS. Vibrate for 30s with an oscillator until the system is uniform. Add 1 ul of the above-mentioned indicator dropwise to the indicator area of ​​the test paper, and place it in the air for 10 minutes. After vacuum packaging, put it in a foil bag and store it away from light.

Embodiment 3

[0033] A preparation of glucose detection test paper, according to the previously designed drawings, the diameter of the indication area is 0.7 cm; the width of the channel is 0.6 cm, and the length is 0.5 cm; the diameter of the liquid area to be tested is 0.6 cm, and the drawing is printed with a paraffin inkjet printer after coming out. Place the test paper in an electric blast drying oven at 120 degrees Celsius and heat it for 7 minutes, then take it out for later use. Add 90ul sol-gel system to a 1ml centrifuge tube, then add 40ul tetramethylbenzidine solution, then add 15ul 2g / L peroxidase in PBS, 4ul 1g / L glucose oxidase in PBS. Vibrate for 30s with an oscillator until the system is uniform. Add 1ul of the above-mentioned indicator dropwise to the indicator area of ​​the test paper, and place it in the air for 20 minutes. After vacuum packaging, put it in a foil bag and store it away from light.

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Abstract

The invention discloses ink-jet printing glucose detection test paper immobilized by using sol-gel. The ink-jet printing glucose detection test paper is prepared by the following steps: uniformly mixing solutions of glucose oxidase, peroxidase and other reactants with sol-gel with enzyme stability, dropwisely adding into an indication area, and carrying out dark drying treatment on the obtained test paper, thereby obtaining the test paper. An indication area, a channel and a to-be-detected liquid area are constructed on the test paper by virtue of a paraffin ink-jet printing technology; when the test paper is used, a certain amount of to-be-detected liquid is dropwise added into the to-be-detected liquid area, the to-be-detected liquid diffuses to the indication area under the capillary action, detection can be performed through color change of the indication area after a period of time, the sol-gel used for immobilization can maintain the activity of enzyme and the shelf life of the test paper is prolonged to 1-2 months. According to the test paper prepared by the method, the consumption of the indicator is extremely low and the cost is greatly saved. The prepared test paper is uniform in color development and the sensitivity is greatly improved.

Description

technical field [0001] The invention relates to the field of medical detection, in particular to an inkjet printing glucose detection test paper immobilized by sol-gel Background technique [0002] Glucose content is a commonly used indicator in biochemical analysis and is often used in the diagnosis of various diseases, such as diabetes. Currently commonly used glucose detection mainly includes two types, namely blood glucose and urine glucose. The former requires blood collection in order to obtain the sample to be tested. The blood drawing process is inevitably accompanied by a tingling sensation. In addition, blood collection requires certain technology and equipment, which is very inconvenient for ordinary users, and this is a great limitation for instant diagnosis. factor. But in all senses, urine sugar testing is much more convenient. Urine collection is non-invasive and naturally does not cause tingling. At present, the dry chemical method urine test paper used i...

Claims

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Application Information

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IPC IPC(8): G01N21/78
CPCG01N21/78
Inventor 冯亮王振名王昱
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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