Cartilage composite scaffold and preparation method thereof
A composite scaffold and cartilage technology, applied in medical science, prosthesis, tissue regeneration, etc., can solve the problems of difficult to meet the patient's exercise requirements, difficult to self-repair, etc., achieve high cartilage damage regeneration and repair effect, promote cartilage regeneration and repair, good The effect of biomechanical properties
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[0061]In step S110, the pine bone is commercially available or prepared by methods known in the art. In some preferred embodiments, the preparation method of the pine bone may include:
[0062]S111, take fresh tubular bones, remove bone, cartilage and soft tissue, take the pine bone portion, and obtain initial cancer.
[0063]In step S111, the tubular bone can be taken from the human body or other animals such as pigs, rabbits, sheep, cattle, and the like. The blade can be used to remove the bone, cartilage and soft tissue of the fresh tubular bone. Further, the initial pine bone can also be prepared into a small piece to facilitate subsequent processing steps. The size and shape of the bone block can be determined according to the actual needs. For example, the bone block can be a circular shape, a cylindrical, a block, and the like. Preferably, the bone block has a thickness of 10 mm or less, such as 2 mm, 5 mm, 8 mm, and the like. As an example, the bone block is a cylindrical shape of...
Embodiment 1
[0152]1, DCB preparation
[0153](1) Pig fresh tubular bone, eliminate bone, cartilage and soft tissue, take the pine bone portion, and prepared a block shape of approximately 10 mm × 10 mm × 10 mm.
[0154](2) The water repeatedly rinsing the bone block, initially removing the bone marrow, blood and surface grease.
[0155](3) Place the bone block in an acidic EOW for 24 hours, further remove bone marrow, blood and surface grease, simultaneously to sterilize.
[0156](4) High pressure water gun rinsing the bone block, further remove bone marrow, blood and surface grease.
[0157](5) Use anhydrous ethanol to dehydrate for 4 h to 6h, air dry, and obtain loose bones.
[0158](6) soak the pine bone in chloroform / methanol (volume ratio 1: 1) solution, and the degreased degreasing 24 h in the thermostatic clocker at 37 ° C, the oscillating speed is 60 rpm.
[0159](7) Soak the pine bone in 75% medical ethanol solution, and washed in a thermostatic clocker at 37 ° C, and the liquid was evolved, and the osci...
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