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Preparation method of amino acid foliar fertilizer

A foliar fertilizer and amino acid technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, nitrogen fertilizer, etc., can solve the problems of poor pertinence, low content of functional nutritional factors, and differences in amino acid components of different crop nutritional characteristics Major problems, to achieve the effect of solving environmental pollution and waste of resources, overcoming the deterioration of amino acids, and improving the high

Inactive Publication Date: 2020-06-02
NANTONG LICHENG BIOLOGICAL ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Amino acid foliar fertilizers currently on the market are mainly made by hydrolyzing protein with acid or protease, which has high production costs, and most products have large differences in amino acid components, high content of ineffective components, and low content of functional nutritional factors. Features are not well targeted

Method used

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  • Preparation method of amino acid foliar fertilizer
  • Preparation method of amino acid foliar fertilizer
  • Preparation method of amino acid foliar fertilizer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Mix and stir corn steep liquor and γ-aminobutyric acid fermentation waste liquid according to the ratio of 1:3, add molasses in a ratio of 3%, and obtain the fermentation medium; The addition ratio is 3.5: 2: 4.5 and mixed uniformly to obtain mixed flora seed liquid; the mixed flora seed liquid is inoculated into the shake flask co-culture medium in an amount of 3%, and the bacterial concentration reaches the logarithmic growth phase to obtain first-class seeds; 8% of the total amount of the first-grade seed strains was inoculated into a 100L seed tank to expand and cultivate to obtain the second-grade seeds; 10% of the total amount of the second-grade seed strains was inoculated into the fermentation medium to ferment, at a temperature of 28°C and a stirring speed of After fermentation and cultivation for 55 hours under the conditions of 120r / min and 3L / min aeration, the fermentation broth was collected. Finally, the fermentation liquid is separated by a decanter centr...

Embodiment 2

[0031]Mix and stir corn steep liquor and γ-aminobutyric acid fermentation waste liquid according to the ratio of 1:10, add molasses at a ratio of 1%, to obtain a fermentation medium; The addition ratio is 5: 2: 3 and the mixed flora seed liquid is uniformly mixed; the mixed flora seed liquid is inoculated into the shake flask co-culture medium in an amount of 5%, and the bacterial concentration reaches the logarithmic growth phase to obtain first-class seeds; 10% of the total bacterial strains of the first-grade seeds are inoculated into a 100L seed tank to expand and cultivate to obtain secondary seeds; 5% of the total bacterial strains of the secondary seeds are inoculated into the fermentation medium for fermentation, at a temperature of 35°C and a stirring speed of After 60 hours of fermentation and cultivation under the conditions of 200r / min and an aeration rate of 5L / min, the fermentation liquid was collected. Finally, the fermentation liquid is separated by a decanter ...

Embodiment 3

[0033] The steps are the same as in Example 1, except that the fermentation temperature is 33°C, the stirring speed is 160r / min, and the time is 58h. After testing, the prepared amino acid foliar fertilizer contains 1.2% of active polypeptide content, 14% of total amino acid content, 0.6% of γ-aminobutyric acid content, 3.5% of glutamic acid content, 3.1% of threonine content, and 3.1% of arginine content. 1.5%, proline content 0.6%, serine content 2%, tyrosine content 0.8%, other amino acid content 3.6%.

[0034] Investigate the content of various amino acids and active polypeptides in the amino acid foliar fertilizer prepared by the mass ratio between the fermentation medium raw material, the fermentation medium raw material, and the mass percentage of added molasses, as follows:

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Abstract

The invention discloses a preparation method of an amino acid foliar fertilizer. The method comprises the following steps: (1) mixing and stirring corn steep liquor and gamma-aminobutyric acid fermentation waste liquid, and adding molasses to obtain a fermentation culture medium; (2) preparing a mixed flora seed solution; (3) inoculating the mixed flora seed solution into a liquid shake flask co-culture medium to obtain primary seeds; (4) inoculating the primary seed strains into a co-culture medium in a seed tank, and carrying out enlarged culture to obtain secondary seeds; (5) inoculating the secondary seed strains into a fermentation culture medium for fermentation; and (6) carrying out centrifuging, separating and filtering on the fermentation liquor to obtain the amino acid foliar fertilizer. According to the mixed flora formula and the efficient secondary fermentation process, the content of total amino acid and functional component gamma-aminobutyric acid in the amino acid foliar fertilizer is high, meanwhile, the harm of gamma-aminobutyric acid fermentation waste liquid to the environment is reduced, and value increase and resource utilization of waste are achieved.

Description

technical field [0001] The invention relates to a preparation method of foliar fertilizer, in particular to a preparation method of amino acid foliar fertilizer. Background technique [0002] γ-aminobutyric acid (γ-aminobutyric acid, GABA) because of its unique physiological functions and a wide range of applications, the market demand is increasing. Microbial fermentation is the mainstream method for preparing GABA, but at the same time, a large amount of fermentation waste liquid is produced, and its main components are various proteins, residual sugars, polypeptides, inorganic salts and other nutrients and residual functional factors GABA. Moreover, the COD concentration of the fermentation waste liquid is as high as 50,000-100,000mg / L, and direct discharge will cause serious environmental pollution, and discharge into the sewage treatment tank will increase the difficulty of treatment and increase the cost of environmental protection investment for enterprises. There is...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G1/00C05G5/23C12N1/20C12N1/16C12R1/11C12R1/085C12R1/74
CPCC05C11/00C12N1/16C12N1/20
Inventor 李古月李江波
Owner NANTONG LICHENG BIOLOGICAL ENG
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