Fucose-rich exopolysaccharide as well as preparation method and application thereof

A technology of exopolysaccharides and fucose, applied in the field of exopolysaccharides, can solve problems such as the structure and application research of uncapsulated exopolysaccharides, and achieve the effect of strong application potential and strong antibacterial activity

Active Publication Date: 2020-06-05
SOUTHWEST UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But there is no in-depth structure and application research on the capsular exopolysaccharide produced by Kosakonia sp.

Method used

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  • Fucose-rich exopolysaccharide as well as preparation method and application thereof
  • Fucose-rich exopolysaccharide as well as preparation method and application thereof
  • Fucose-rich exopolysaccharide as well as preparation method and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1, the preparation of microbial exopolysaccharide (EPS)

[0031] EPS is fermented and produced by Kosakonia sp.CCTCC M2018092 strain under fed-batch conditions. The specific steps are as follows: take Kosakonia sp.CCTCC M2018092 strain and culture it in a 250mL shake flask containing 30mL medium at 30°C and 200rpm for 20 hours , and then 30ml of bacterial culture solution was transferred to a 15L size fermenter at 30°C and 300rpm for pre-growth culture for 13 hours (air flow 1.5m 3 / h). Afterwards, get 3L pre-growth bacterial liquid and transfer in the 50L fermenter (containing 30L culture medium) to carry out fed-batch fermentation. According to the amount of residual sugar, a peristaltic pump was used to feed in 200 g / L glucose solution in batches at a rate of 0.9-3.8 rpm 13 hours after the fermentation started. The ventilation volume of the fermenter is 1.5m 3 / h, the dissolved oxygen concentration is controlled above 10% by automatically adjusting the s...

Embodiment 2

[0037] Embodiment 2, the structural identification of AH-EPS

[0038] The weight average molecular weight (Mw) of EPS and AH-EPS was measured by gel permeation chromatography (GPC). A PL-GPC50 GPC Integrated System (Agilent) equipped with a PLAquagel-OH mixed-H 8 μm column and a differential detector was used. at 30 °C with 0.1 M NaNO 3 and 500ppm NaN 3 A sample with an appropriate concentration is separated as an eluent. The results showed that the EPS produced from Kosakonia sp.CCTCC M2018092 is a heterogeneous high molecular weight polysaccharide with a Mw of about 3.65×10 5 Da (Mw / Mn=1.7). AH-EPS is a homogeneous EPS with an average mass of 3.47×10 4 Da (Mw / Mn=1.2). Due to the advantages of large-scale production and homogeneity of AH-EPS, a comprehensive structural characterization of AH-EPS was carried out.

[0039] 1. High-performance liquid chromatography (HPLC) analysis of glycan composition

[0040] The monomer composition of AH-EPS was determined after compl...

Embodiment 3

[0073] Preparation and antibacterial test of embodiment 3, EPS / nanometer silver film

[0074] AH-EPS solutions with different concentrations (0.01mg / mL, 0.03mg / mL, 0.05mg / mL, 0.10mg / mL, 0.20mg / mL, 0.50mg / mL) and 2mM AgNO 3 The solution was mixed into 5 mL of water and shaken for 30 minutes. Afterwards, the solution was irradiated under a 354nm UV lamp for 12 minutes to prepare silver nanoparticles, and the nano-silver solution was added (0mL, 1.5mL, 3.0mL, 6.0mL) to 25mLEPS (1.5wt%) solution, and then the solution was poured Put it into a plastic flat plate (d=6.5cm), and form a film after drying at 50°C for 12h. Using the Kirby-Bauer method, the films were peeled off and cut into slice shapes (d=0.5 cm) for antibacterial testing, using Gram-positive Staphylococcus aureus (ATCC29213) as the test bacteria. The absorption spectra of silver nanoparticles solutions prepared by different concentrations of AH-EPS are as follows: Figure 9 Shown in A. The results showed that when...

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Abstract

The invention discloses fucose-rich exopolysaccharide as well as a preparation method and application thereof. The L-fucose, D-glucose, D-galactose, D-glucuronic acid and a pyruvic acid unit are usedas raw materials. Molar ratio of 2.03: 1 1.00: 1.18: 0.64: 0.67, mw is 3.65 * 10 < 5 > Da; the strain is prepared by fermenting a Kosakonia sp. CCTCC (China Center For Type Culture Collection) M2018092 strain, the acid hydrolysis exopolysaccharide has reducibility and can be used as a reducing agent and a stabilizing agent, nano-silver with uniform size is prepared through a green method, the EPS/nano-silver antibacterial film is further prepared, and the prepared film has an antibacterial effect and has good application potential in the aspect of biodegradable antibacterial films.

Description

technical field [0001] The invention relates to exopolysaccharides, in particular to exopolysaccharides rich in fucose, and to a preparation method and application of the exopolysaccharides. Background technique [0002] Natural polysaccharides are ubiquitous high molecular weight biopolymers secreted by plants or microorganisms during growth. Recently, microbial exopolysaccharides (EPS) with high production capacity and diverse properties have been extensively studied. EPS secreted by bacteria, yeasts, fungi or molds vary greatly in composition, and EPS contains one or more monosaccharide residues, which are linked by straight or branched chains. Studies have shown that different components make different EPS have different application potentials in the fields of pharmaceuticals, nutrition and health care, functional foods and cosmetics. For example, xanthan gum has been used in the food industry, personal care products and pharmaceutical industries as a thickener, stabil...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A01N43/16A01N59/16A01P1/00C12P19/04C12R1/01
CPCA01N43/16A01N59/16C08B37/0003C08B37/006C12P19/04
Inventor 邹祥李姗姗夏海钦谢爱卿
Owner SOUTHWEST UNIVERSITY
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