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Water-soluble cross-linking agent, preparation method thereof and prepared electrospun collagen fibers

A cross-linking agent, water-soluble technology, applied in the field of electrospun collagen fiber preparation, can solve the problems of poor electrospinning, poor structural stability of electrospun collagen fibers, difficult molecular chain entanglement, etc., and achieves increased chain entanglement and good structure. Effects of stability and mechanical properties

Active Publication Date: 2020-06-05
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the above-mentioned deficiencies in the prior art, the present invention provides a water-soluble cross-linking agent and its preparation method and the prepared electrospun collagen fiber, which can effectively solve the problem that the existing collagen has poor electrospinning property and is difficult to form sufficient molecular chains. Entanglement, the problem of poor structural stability of electrospun collagen fibers

Method used

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  • Water-soluble cross-linking agent, preparation method thereof and prepared electrospun collagen fibers
  • Water-soluble cross-linking agent, preparation method thereof and prepared electrospun collagen fibers
  • Water-soluble cross-linking agent, preparation method thereof and prepared electrospun collagen fibers

Examples

Experimental program
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Effect test

Embodiment 1

[0039] A preparation method of electrospun collagen fibers, comprising the following steps:

[0040] (1) Dissolve 0.025 parts of aldehyde-containing norbornene-based hydrophobic monomer NCHO and 0.5 part of norbornene-based hydrophilic monomer NTEG containing triethylene glycol structure in 0.25 and 5 parts of dry dichloromethane, respectively ;

[0041] (2) the 3rd generation catalyst of 0.01 part of Grubbs is dissolved in the flask that 0.3 part of dry dichloromethane is housed under nitrogen protection; Stir vigorously at room temperature and react under nitrogen protection for 20 minutes;

[0042] (3) Add the norbornene-based hydrophilic monomer solution containing triethylene glycol structure into the reaction system quickly, and further react vigorously at room temperature for 20 minutes; then inject 0.5 parts of vinyl ether into the flask to deactivate the catalyst, Diethyl ether was used to promote product precipitation, and the precipitate was collected by filtratio...

Embodiment 2

[0046] A preparation method of electrospun collagen fibers, comprising the following steps:

[0047] (1) Dissolve 0.05 parts of aldehyde-containing norbornene-based hydrophobic monomer NCHO and 1 part of norbornene-based hydrophilic monomer NTEG containing triethylene glycol structure in 0.5 and 10 parts of dry dichloromethane, respectively ;

[0048] (2) the 3rd generation catalyst of 0.015 part of Grubbs is dissolved in the flask that 0.4 part of dry dichloromethane is housed under nitrogen protection; Stir vigorously at room temperature and react under nitrogen protection for 10 minutes;

[0049] (3) Add the norbornene-based hydrophilic monomer solution containing triethylene glycol structure quickly into the reaction system, further react vigorously at room temperature for 10 minutes, then inject 0.5 parts of vinyl ether into the flask to deactivate the catalyst, Diethyl ether was used to promote product precipitation, and the precipitate was collected by filtration, com...

Embodiment 3

[0053] A preparation method of electrospun collagen fibers, comprising the following steps:

[0054] (1) Dissolve 0.04 parts of aldehyde-containing norbornene-based hydrophobic monomer NCHO and 0.8 parts of norbornene-based hydrophilic monomer NTEG containing triethylene glycol structure in 0.4 and 8 parts of dry dichloromethane, respectively ;

[0055] (2) the 3rd generation catalyst of 0.012 parts of Grubbs is dissolved in the flask that 0.32 parts of dry methylene chloride is housed under nitrogen protection; Stir vigorously at room temperature and react under nitrogen protection for 15 minutes;

[0056] (3) Add the norbornene-based hydrophilic monomer solution containing triethylene glycol structure quickly into the reaction system, further react vigorously at room temperature for 15 minutes, then inject 0.5 parts of vinyl ether into the flask to deactivate the catalyst, And use diethyl ether to promote the precipitation of the product, filter to take the precipitate, an...

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Abstract

The invention discloses a water-soluble cross-linking agent, a preparation method of the water-soluble cross-linking agent and an electrospun collagen fiber prepared from the water-soluble cross-linking agent. The preparation method of the cross-linking agent comprises the following steps: (1) in a protective gas atmosphere, mixing an aldehyde group-containing norbornene hydrophobic monomer solution with a catalyst solution, carrying out a stirring reaction at room temperature for 10-20 min, then adding a triethylene glycol-containing norbornene hydrophilic monomer, and continuing the stirringreaction at room temperature for 10-20 min; and (2) adding a reaction terminator to terminate the reaction, adding an organic reagent to precipitate a reactant, filtering, and purifying and drying the solid-phase product. The cross-linking agent prepared by the invention can improve the stability of collagen, so that the prepared electrospun collagen nanofiber can also have better structural stability and mechanical properties.

Description

technical field [0001] The invention belongs to the technical field of preparation of electrospun collagen fibers, and in particular relates to a water-soluble crosslinking agent, a preparation method thereof, and the prepared electrospun collagen fibers. Background technique [0002] Collagen is the main component of the extracellular matrix and the most abundant structural protein in animals. It has inherent advantages in the preparation of biomedical materials such as tissue engineering scaffolds and drug carriers. However, the thermal stability and mechanical properties of natural collagen are often difficult to meet the needs of practical applications. Certain modification methods are often used to endow collagen with better structural stability and functional properties. Among them, chemical crosslinking is one of the most effective means of modification. At present, the chemical cross-linking agents for collagen reported in the literature mainly include glutaraldehyd...

Claims

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Application Information

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IPC IPC(8): C08F232/08D01F4/00D01F1/10
CPCC08F232/08D01F1/10D01F4/00
Inventor 刘文涛李培玉白聪辜海彬
Owner SICHUAN UNIV
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