Identification method of soybean rhizobium strain 5873 pcr, its primers and application

A technology of 5873PCR and soybean rhizobia, which is applied in biochemical equipment and methods, microbial measurement/testing, DNA/RNA fragments, etc., can solve the problems of intellectual property protection of unfavorable production strains, cumbersome process, poor repeatability, etc.
CN111235291BActive Publication Date: 2022-05-13INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
Publication Date
2022-05-13

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Abstract

The invention relates to a PCR identification method for soybean rhizobium strain 5873, primers used therefor and applications, belonging to the technical field of bacterial strain identification. The method comprises: using the genomic DNA of the bacterial strain to be tested or the pure culture bacterial liquid of the bacterial strain or the root nodule crushing extract after cracking, boiling water bath, and freeze-thawed liquid as a template, and performing PCR amplification with primer 4-4; Perform electrophoresis on the PCR product, and if there is no band of about 355bp, it is not soybean rhizobium strain 5873; if there is a band of about 355bp, continue to use primer Q1 for PCR amplification of the strain to be tested; perform electrophoresis on the above product, and there is a band of about 218bp Soybean Rhizobium strain 5873. The present invention has the following advantages: In terms of strain identification and evaluation, soybean rhizobia strain 5873 can be easily and quickly distinguished, and bacteria or nodules can be directly used as templates, eliminating the cumbersome separation, cultivation, and DNA extraction and identification of nodules. link.
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Description

technical field

[0001] The invention belongs to the technical field of strain identification, and in particular relates to a PCR identification method of soybean rhizobium strain 5873, primers used therein and applications. Background technique

[0002] With the adjustment of the country's agricultural strategy, the development of resource-saving and environment-friendly "two-type agriculture" has become the only way for the sustainable development of modern agriculture. Among them, microbial fertilizers including rhizobia, as new green inputs and priority biological products, can not only repair and improve soil, reduce weight and increase efficiency, but also perform outstandingly in improving crop quality and efficiency. The action plan of reducing one increase provides an important guarantee for the realization of the goal of "one control, two reductions and three basics".

[0003] As an important functional strain in microbial fertilizer products, soybean rhizobia can ...

Claims

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