System and method for researching bioelectrical effect of high-frequency electromagnetic radiation on in-vitro excitable cells

Abstract

The invention relates to a system and a method for researching the bioelectricity effect of high-frequency electromagnetic radiation on in-vitro excitable cells. Terahertz waves are designed and constructed from top to bottom by utilizing an electrophysiological recording system and a microscope observation system for adherent growth culture cells, synchronous detection of real-time irradiation terahertz and electrophysiological characteristics of adherent growth in-vitro culture neurons and other excitable cells can be realized, and the system is simple to operate and high in repeatability. On one hand, a terahertz source is integrated into an electrophysiological recording system and is fixed on an electric three-dimensional micromanipulator, so that the terahertz source can be moved freely and accurately; meanwhile, terahertz waves invisible to naked eyes are combined with the visible light laser positioning sighting device, and the irradiation position of the terahertz waves is determined. And on the other hand, the attenuation degree of terahertz waves in the liquid is reduced by controlling the liquid level height of the electrophysiological recording chamber, namely the culture dish, so that the neurons are irradiated in real time, and electrophysiological indexes are recorded in real time.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to a system and method for studying the bioelectric effect of high-frequency electromagnetic radiation on excitable cells in vitro. Background technique [0002] Terahertz (THz) waves refer to the electromagnetic spectrum region between millimeter waves and infrared light with a frequency range of 0.1 to 10 THz and a wavelength of 3 mm to 30 μm. It is the only last spectrum interval in the electromagnetic spectrum that has not been fully studied and well utilized. It is a "blank" area of ​​the electromagnetic spectrum that has not been fully developed by humans. In terms of energy, terahertz waves are between photons and electrons, and it has many unique properties: ①High permeability, THz has good penetration to many dielectric materials and non-polar substances, such as ceramics and carbon plates , plastics, etc.; ②Low energy, THz photon energy is 4.1meV (millielectron volts), only 10 ...

AI Technical Summary

Problems solved by technology

However, there is still a lack of systems and methods for studying the bioelectric effects of terahertz real-time irradiation and simultaneous detection on excitable cells in vitro, and it is urgent to develop

[0004] However, for the ex vivo culture of excitable cells under instant terahertz irradiation and the simultaneous study of their bioelectric effects, there are the following difficulties: (1) How to organically integrate the terahertz irradiation system into the electrophysiological recording system to ensure that the terahertz irradiation system can be used from time to time Controllable irradiation of isolated neurons in terms of , amplitude, etc., and the electrophysiological recording system can work normally to obtain bioelectrical signals; (2) Terahertz waves are invisible to the naked eye, and the equipment that can be used to detect the spatial distribution of terahertz waves is complex , the space in the existing equipment for detecting cell bioelectrical effects is limited, and it is difficult to integrate the two. How to ensure that the terahertz wave is irradiated to the designated position, which is convenient for electrophysiological recording; The survival of cells depends on the liquid environment, how to ensure that more terahertz waves can be irradiated on the cells as much as possible

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