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Reaction cup, molecular hybridization instrument, application of reaction cup and detection method

A technology of reaction cups and reaction plates, which is applied in the field of molecular hybridization instruments and detection, can solve the problems of increasing the risk of reaction system contamination, increasing the complexity of taking and placing membrane strips, complexity and high cost, and saving desktop space, saving overall Machine space, reduce design complexity and cost

Pending Publication Date: 2020-06-09
ACON BIOTECH (HANGZHOU) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the addition of the tank cover increases the complexity of the operation when taking and placing the film strips.
If the tank cover is not tightly sealed, the liquid in the cuvette may still overflow, increasing the risk of contamination of the reaction system
On the other hand, the horizontal reaction tank adopts a linear reciprocating mechanism, which is complex in design and high in cost, and the reliability of the mechanism movement is poor.

Method used

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  • Reaction cup, molecular hybridization instrument, application of reaction cup and detection method
  • Reaction cup, molecular hybridization instrument, application of reaction cup and detection method
  • Reaction cup, molecular hybridization instrument, application of reaction cup and detection method

Examples

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Comparison scheme
Effect test

Embodiment 1

[0054] Example 1 Application of the cuvette 10 of the present invention in an automatic nucleic acid molecular hybridization instrument.

[0055] Such as Image 6 As shown, the main components of the automatic nucleic acid molecular hybridization instrument include: a reaction plate 1, a liquid injection needle 2, a lifting mechanism 3, a liquid suction needle 4, a liquid injection pump 6 and a reagent bottle 7.

[0056] The working process of the automatic nucleic acid molecular hybridization instrument is described as follows:

[0057] 1. Place the cuvette 10 of the present invention with the film strip 20 in the cuvette on the reaction plate 1 . 48 cuvettes 10 can be placed in the instrument described in this embodiment.

[0058] 2. The instrument uses the injection pump 6 to inject the solution I in the reagent bottle 7 into the cuvette 10 through the injection needle 2 .

[0059] 3. After the solution I is injected into the cuvettes 10, the samples to be analyzed are s...

Embodiment 2

[0092] Example 2 Detection of human papillomavirus genotypes in samples by reverse dot hybridization

[0093] The membrane strip immobilized with 14 high-risk HPV genotype-specific probes is placed vertically in the upright position of the membrane strip of the cuvette of the present invention. The 14 high-risk types: HPV16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 68, and 73. Place the cuvette with the film strip in the cup groove on the reaction plate of the automatic nucleic acid molecular hybridization instrument, turn on the automatic nucleic acid molecular hybridization instrument, and the working process of the hybridization instrument is carried out in the manner of Example 1. After the hybridization instrument finishes the working process described in Example 1, the membrane strip is taken out, and it is judged whether the detected sample contains the existence of these HPV genotypes according to the hybridization signal on the membrane strip. The detection result...

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Abstract

The invention provides a reaction cup. The reaction cup comprises a cup wall and a cup bottom; the cup wall is further provided with retaining parts; the retaining parts can make dot blot membranes stand upright in the reaction cup, and a dot blot membrane upright placement position is formed in a region of connecting lines of two opposite retaining parts. The invention further provides a nucleicacid molecular hybridization instrument comprising the reaction cup, and a detection method for detecting an analyte. The new reaction cup provided by the invention can vertically hold the dot blot membranes, and can be operated in an open state, so that the area of the reaction cup on the molecular hybridization instrument is reduced; and more reaction cups can be placed on the molecular hybridization instrument once, so that the working efficiency of the molecular hybridization instrument to detect the analyte is improved.

Description

technical field [0001] The invention relates to the technical field of molecular hybridization, in particular to a cuvette applicable to a molecular hybridization instrument, a molecular hybridization instrument using the cuvette, and a detection method. Background technique [0002] Nucleic acid molecular hybridization is one of the most basic methods of genetic diagnosis. Its basic principle is: complementary DNA single strands can be combined into double strands under certain conditions, that is, capable of hybridization. This combination is specific, that is, it is carried out strictly according to the principle of base complementarity, and it can not only be carried out between DNA and DNA, but also between DNA and RNA. Therefore, when a nucleic acid sequence of a known gene is used to make a probe and contact with the denatured single-stranded genomic DNA, if the bases of the two are completely paired, they will complementarily combine into a double strand, thereby in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/00C12Q1/6834
CPCC12Q1/6834C12Q2565/625C12Q1/708B01L2200/025B01L3/5082B01L2300/0825B01L3/0293B01L9/06B01L2200/141B01L2300/0609B01L2300/0851B01L2200/16C12Q1/6837
Inventor 张昊史亮羊益刚赵岩王志恒朱秀峰王志海缪国超谢小恒
Owner ACON BIOTECH (HANGZHOU) CO LTD
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