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A method for raising seedlings of Astragalus membranaceus with high content of astragaloside IV

A technology of astragaloside IV content, applied in the field of plant cultivation, can solve problems such as quality degradation, soil micro-ecological damage, and "pesticide residue" exceeding the standard, and achieve the effects of preventing pollution, improving survival rate, and improving permeability

Inactive Publication Date: 2021-09-21
内蒙古自治区生物技术研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to production needs, "continuous stubble" planting is usually used, so a large amount of chemical fertilizers and pesticides have to be used, resulting in serious damage to soil micro-ecology, quality degradation, and excessive "pesticide residues"

Method used

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  • A method for raising seedlings of Astragalus membranaceus with high content of astragaloside IV
  • A method for raising seedlings of Astragalus membranaceus with high content of astragaloside IV

Examples

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Effect test

Embodiment 1

[0026] A method for cultivating Astragalus seedlings with high content of astragaloside IV, comprising the following steps:

[0027] (1) Take an appropriate amount of Astragalus mongolica seeds, add 4 times the weight of water, add an appropriate amount of sodium hydroxide to adjust the pH value to 12, treat at room temperature for 60 minutes, pour off the water and leave the seeds;

[0028] (2) Add 70% ethanol solution to the astragalus seeds in step (1), after ultrasonic treatment time 40s; wash with water 5 times;

[0029] (3) Add 4 times the weight of 8% sodium hypochlorite solution to the Astragalus seeds in step (2) and soak for 15 minutes, wash with water 5 times and drain;

[0030] (4) Put the Astragalus membranaceus seeds in step (3) into the first MS medium for cultivation, the cultivation temperature is 20° C., the humidity is 60%, and they are cultivated for 12 days to germinate, and the first MS medium is replaced at intervals of 4 days;

[0031] Each liter of th...

Embodiment 2

[0035] A method for cultivating Astragalus seedlings with high content of astragaloside IV, comprising the following steps:

[0036] (1) Take an appropriate amount of Astragalus membranaceus seeds, add 3 times the weight of water, add an appropriate amount of potassium hydroxide to adjust the pH value to 11, treat at room temperature for 50 minutes, pour off the water and leave the seeds;

[0037] (2) Add 70% ethanol solution to the astragalus seeds in step (1), after ultrasonic treatment time 60s; wash with water 5 times;

[0038] (3) Add 4 times the weight of 1% mercuric chloride solution to the Astragalus seeds in step (2) and soak for 15 minutes, wash with water 5 times and drain;

[0039] (4) Put the Astragalus membranaceus seeds in step (3) into the first MS medium for cultivation, the cultivation temperature is 20° C., the humidity is 50%, and they are cultivated for 12 days to germinate, and the first MS medium is replaced at intervals of 4 days;

[0040]Each liter of...

Embodiment 3

[0044] A method for cultivating Astragalus seedlings with high content of astragaloside IV, comprising the following steps:

[0045] (1) Take an appropriate amount of Astragalus mongolica seeds, add 3 times the weight of water, add an appropriate amount of sodium hydroxide to adjust the pH value to 10, treat at room temperature for 40 minutes, pour off the water and leave the seeds;

[0046] (2) Add 60% ethanol solution to the astragalus seeds in step (1), after ultrasonic treatment time 40s; wash with water 4 times;

[0047] (3) Add 4 times the weight of 8% sodium hypochlorite solution to the Astragalus seeds in step (2) and soak for 10 minutes, wash with water 4 times and drain;

[0048] (4) Put the Astragalus membranaceus seeds in the step (3) into the first MS medium for cultivation, the cultivation temperature is 25° C., the humidity is 55%, and they are cultivated for 8 days to germinate, and the first MS medium is replaced at intervals of 4 days;

[0049] Each liter of...

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Abstract

The invention provides a method for growing seedlings of Astragalus membranaceus with high content of astragaloside IV. Astragalus seeds are soaked in alkaline solution and high-concentration ethanol, and ultrasonically treated to improve the germination rate of Astragalus seeds, so that the seedlings have strong vitality; and by applying 5- 8% sodium hypochlorite solution or 1% mercuric chloride solution, aseptic water is selected, and the medium is sterilized to provide a sterile growth environment, which greatly improves the survival rate of seedlings; at the same time, through The application of different MS medium during the germination of Astragalus seeds and different stages of seedling growth, especially the application of N elements and trace organic substances (inositol) during the seedling growth stage, greatly increased the content of astragaloside IV.

Description

technical field [0001] The invention relates to a method for raising plant seedlings, in particular to a method for raising astragalus seedlings with high content of astragaloside IV, and belongs to the technical field of plant cultivation. Background technique [0002] Astragalus is a perennial herb belonging to the leguminous family, and its roots are used as medicine. The main pharmacological effects of Astragalus include invigorating qi and strengthening the surface, diuresis, detoxification and expulsion of pus, and promoting muscle growth. It is mainly used to treat shortness of breath, collapse, palpitations, spontaneous sweating, physical weakness and edema, chronic nephritis, dehydration, chronic diarrhea, qi deficiency, and insufficient qi and blood. , Sinking of Qi, has antibacterial and anti-inflammatory, antihypertensive and diuretic, detoxifying and choleretic effects, and is a commonly used Chinese herbal medicine. The domestic market has a great demand for A...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G31/00A01C1/00A01C1/08A01G24/10A01G24/30C05G5/23C05G1/00
CPCA01C1/00A01C1/08A01G31/00A01G24/10A01G24/30C05B7/00
Inventor 白朕卿张秀娟随洋吴佳文田雨王睿陈启渊焦巍
Owner 内蒙古自治区生物技术研究院