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Microscopic breeding method for aurelia aurita hydranth in laboratory

A polyp and laboratory technology, which is applied in the field of microscopic feeding of the hydra of the sea moon jellyfish in the laboratory, can solve the problem that the constant temperature control of the water tank does not meet the standard of experimental animal breeding, and the scientific research experiment of accurate quantitative polyp cannot be carried out. , large water body in the breeding tank, etc., to achieve the effects of easy promotion, saving experimental costs, and fast reproduction

Active Publication Date: 2020-06-19
THE NAVAL MEDICAL UNIV OF PLA
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Regarding the breeding of polyps, the general feeding method in the laboratory is the feeding method of corrugated plate attachment + circulating filter water tank, but this feeding method is relatively rough and has obvious defects: first, the water tank is under constant temperature control, precise and quantitative feeding, and water quality. Aspects such as purification all can't reach the standard that experimental animal is raised; Secondly, the traditional way of raising is because the water body of rearing tank is too big, and the polyp body quantity that corrugated plate is attached is numerous (referring to figure 1 ) and other shortcomings, resulting in the inability to carry out accurate and quantitative scientific research experiments related to polyps

Method used

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  • Microscopic breeding method for aurelia aurita hydranth in laboratory
  • Microscopic breeding method for aurelia aurita hydranth in laboratory
  • Microscopic breeding method for aurelia aurita hydranth in laboratory

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Embodiment 1 6

[0035] The feeding of the moon jellyfish polyp in the six-hole cell culture plate of embodiment 1

[0036] 1. Preparation of artificial seawater suitable for the survival of the polyps of the moon jellyfish

[0037] Prepare artificial seawater with a theoretical salinity of 3.00% according to Table 1:

[0038] Table 1 Preparation table of artificial seawater (salinity is about 3.00%)

[0039]

[0040] After the configuration is complete, turn on the air pump and extend it into the prepared artificial seawater to pump air into the seawater. After 2 hours, take out the air pump. Use a salinity meter to measure the salinity between 2.8 and 3.2 (about 3.00%), add seawater or distilled water appropriately according to the salinity, let it settle for 5 minutes, and take the supernatant for use.

[0041] 2. Preparation of the bait for the polyp of the sea moon jellyfish (brine shrimp Artemia)

[0042] Pour the prepared 1L of artificial seawater and appropriate amount of brine s...

Embodiment 2

[0064] The raising of jellyfish polyp in embodiment 2 petri dish

[0065] The difference between this embodiment 2 and embodiment 1 is only in step 3. After the impurities attached to the polyp are removed, the cleaned polyp is sucked into a clean beaker equipped with artificial seawater, and it is left to stand for 30 minutes. To restore the state, select the polyps with stretched tentacles and good vitality and put them into a petri dish filled with artificial seawater. There are 20 to 30 polyps in each culture plate, with the feet down and the tentacles up or sideways. Put the lid on, and put a label on the lid to record the date of planting, such as image 3 As shown, A and B show the polyps of the jellyfish with different planting dates.

[0066] According to the comparison of the hydra of the jellyfish and the hydra of the jellyfish raised in the embodiment of the present invention 1 and 2 and the traditional feeding method Figure 4 shown, according to Figure 4 , th...

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Abstract

The invention relates to the technical field of hydranth breeding in a laboratory, and provides a microscopic breeding method for aurelia aurita hydranth in a laboratory. The microscopic breeding method comprises seven steps of artificial seawater preparation, bait preparation, aurelia aurita hydranth transfer, aurelia aurita hydranth attachment, aurelia aurita hydranth feeding, artificial seawater replacement and subculture. According to the method, a six-hole culture plate or a single culture dish is selected as a culture utensil, the number of aurelia aurita hydranths is controllable and countable, and operations such as feeding and water replacement are convenient and easy to reach the standard; meanwhile, a biochemical incubator with higher control precision is selected as a culture instrument, on one hand, the problem that a current laboratory breeding water tank cannot reach the standard of experimental animal breeding in the aspects of constant temperature control, accurate quantitative feeding, water quality purification and the like is solved; and on the other hand, hydranth quantification and subculture are easy, the vitality of the hydranths is improved, and furthermore, the problem that a traditional breeding mode cannot carry out accurate quantitative scientific research experiments related to the hydranths is solved.

Description

technical field [0001] The invention belongs to the field of laboratory cultivation of polyps of jellyfish jellyfish, and in particular relates to a microcosmic breeding method of polyps of jellyfish jellyfish in a laboratory. Background technique [0002] The polyp is the most important generation in the growth cycle of the jellyfish of the cnidaria phylum. It is an invertebrate, and it is mostly found in seawater, and a few species are produced in freshwater. The polyp of the jellyfish is often used as an animal model for basic research because of its simple biological structure, fast reproduction speed, and strong regeneration ability. Therefore, the polyps of the jellyfish are a class of animals with great research potential in the fields of biology, chemistry, medicine and other scientific research. [0003] Regarding the breeding of polyps, the general feeding method in the laboratory is the feeding method of corrugated plate attachment + circulating filter water tank...

Claims

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Application Information

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IPC IPC(8): A01K61/20A01K61/59A23K50/80A23K10/22A01K63/00A01K63/04A01K61/17
CPCA01K61/20A01K61/59A23K50/80A23K10/22A01K63/003A01K63/04A01K61/17Y02A40/81
Inventor 肖良陈新彤梁洪昱刘文文李晓亚姜贵先李新书崔建国张晶徐拯
Owner THE NAVAL MEDICAL UNIV OF PLA
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