Preparation method and application of CHIR99021 and FGF1 nanoparticle combined myocardial tissue block

A technology of CHIR99021, 1. CHIR99021 is applied in the application field of treating ischemic heart disease in animals, and can solve problems such as the survival rate of small myocardial block transplantation

Pending Publication Date: 2020-06-19
THE SECOND XIANGYA HOSPITAL OF CENT SOUTH UNIV
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  • Abstract
  • Description
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Problems solved by technology

[0004] Although human induced pluripotent stem cell (hiPSC) technology and heart tissue engineered myocardial patch (Patch)-based methods have made significant medical progress in cell therapy of myocardial infarction, most hiPSC-CMs myocardial patches are small and the graft survival rate is too high. Low

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  • Preparation method and application of CHIR99021 and FGF1 nanoparticle combined myocardial tissue block
  • Preparation method and application of CHIR99021 and FGF1 nanoparticle combined myocardial tissue block
  • Preparation method and application of CHIR99021 and FGF1 nanoparticle combined myocardial tissue block

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Embodiment Construction

[0047] The following examples are intended to further illustrate the present invention, rather than limit the present invention.

[0048] 1. Materials and methods

[0049] 1 Experimental materials

[0050] 1.1 Experimental animals

[0051] The experimental animals were 16-week-old female NOD-SCID mice with a body weight of 25-30 g, which were purchased from Jackson Company. Further husbandry and breeding were performed at the Animal Care Center, Department of Biomedical Engineering, University of Alabama at Birmingham.

[0052] 1.2 Experimental cells

[0053] hiPSCs are re-encoded from cardiac fibroblasts of human volunteers. Four transcription factors, Sox2, Klf4, Oct3 / 4 and C-Myc, are transfected into the above-mentioned cardiac fibroblasts by using lentivirus as a vector, and then re-encoded into hiPSCs.

[0054] 1.3 Main reagents, antibodies and instruments

[0055] List of experimental reagents

[0056]

[0057]

[0058] List of experimental antibodies (primary...

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Abstract

The invention aims to provide a preparation method and application of a CHIR99021 and FGF1 nanoparticle combined myocardial tissue block. The preparation method of the CHIR99021 and FGF1 nanoparticlecombined myocardial tissue block comprises the following steps of: 1) preparing CHIR99021 and FGF1 nanoparticles; and 2) preparing a human induced pluripotent stem cell-derived myocardial tissue block(iCM-C + F-NP Patch) containing the CHIR99021 and FGF1 nanoparticles. The prepared human induced pluripotent stem cell-derived myocardial tissue block (iCM-C + F-NP Patch) containing the CHIR99021 and FGF1 nanoparticles is applied to treatment of animal ischemic heart disease. The CHIR99021 and FGF1 are combined for use, so that proliferation of hiPSC-CMs can be promoted and apoptosis can be reduced through synergistic effect; when the CHIR99021 and FGF1 are used for treating the animal ischemic heart disease, more hiPSC-CMs can be survived after transplantation, myocardial infarction can beeffectively relieved, periodical activity of myocardial infarction transplantation cells can be promoted, angiogenesis can be promoted, apoptosis reaction can be reduced, and the CHIR99021 and FGF1 nanoparticle combined myocardial tissue block can be applied to clinical treatment of the myocardial infarction.

Description

technical field [0001] The invention belongs to the technical field of cell therapy, in particular to the PLGA nanoparticles of two small molecule drugs CHIR99021 and FGF1 and the preparation method of human induced pluripotent stem cell-derived myocardial (hiPSC-CMs) tissue blocks and their application in the treatment of ischemic diseases in animals. Applications in Cardiology. Background technique [0002] Ischemic heart disease, caused by coronary artery blockage, accounts for 80% of cardiovascular disease deaths. The limited regeneration of myocardium in the adult mammalian heart and the shortage of donor hearts are major obstacles to conventional cardiac therapy. In the field of cardiovascular tissue engineering, the preparation and / or regeneration of engineered myocardium is the most difficult task. FGF1, also known as acidic fibroblast growth factor (αFGF), is involved in a range of physiological processes including development, morphogenesis, wound healing and cel...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/18A61K31/506A61K35/545A61K9/14A61K47/34A61P9/10B82Y5/00
CPCA61K38/1825A61K31/506A61K35/545A61K9/146A61P9/10B82Y5/00A61K2300/00
Inventor 范成铭吴逊杨进福
Owner THE SECOND XIANGYA HOSPITAL OF CENT SOUTH UNIV
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