Method for detecting PBMC activity under in-vitro 3D culture condition by using resazurin reagent
A culture condition, resazurin technology, applied in biochemical equipment and methods, measuring devices, and microbial determination/inspection, etc., can solve problems such as limited cell yield, and achieve the effects of convenient operation and high detection sensitivity
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Embodiment 1
[0025] A method for detecting PBMC activity under in vitro 3D culture conditions using a resazurin reagent, the specific steps are as follows:
[0026] Step 1, preparation of resazurin mother liquor: take 1 g of resazurin sodium and add 200 mL of three-distilled water to prepare a solution with a final concentration of 0.5%, and use a disposable filter with a pore size of 0.22 μm to sterilize the resazurin solution. Then put the sterilized resazurin solution into a 4°C refrigerator and store it for later use;
[0027] Step 2, preparation of resazurin detection reagent: according to the needs of the experiment, take an appropriate amount of resazurin mother solution, add a certain amount of three-distilled water, and prepare a resazurin solution with a final concentration of 0.15%, which is the resazurin detection Reagent;
[0028] Step 3: Determination of PBMC activity under in vitro 3D culture conditions: according to the ratio of the volume of the 3D agarose hydrogel medium...
Embodiment 2
[0031] A method for detecting PBMC activity under in vitro 3D culture conditions using a resazurin reagent, the specific steps are as follows:
[0032] Step 1, preparation of resazurin mother liquor: take 1 g of resazurin sodium and add 200 mL of three-distilled water to prepare a solution with a final concentration of 0.5%, and use a disposable filter with a pore size of 0.22 μm to sterilize the resazurin solution. Then put the sterilized resazurin solution into a 4°C refrigerator and store it for later use;
[0033] Step 2, preparation of resazurin detection reagent: according to the needs of the experiment, take an appropriate amount of resazurin mother solution, add a certain amount of three-distilled water, and prepare a resazurin solution with a final concentration of 0.17%, which is the resazurin detection Reagent;
[0034]Step 3: Determination of PBMC activity under in vitro 3D culture conditions: according to the ratio of the volume of the 3D methylcellulose hydrogel...
Embodiment 3
[0037] A method for detecting PBMC activity under in vitro 3D culture conditions using a resazurin reagent, the specific steps are as follows:
[0038] Step 1, preparation of resazurin mother liquor: take 1 g of resazurin sodium and add 200 mL of three-distilled water to prepare a solution with a final concentration of 0.5%, and use a disposable filter with a pore size of 0.22 μm to sterilize the resazurin solution. Then put the sterilized resazurin solution into a 4°C refrigerator and store it for later use;
[0039] Step 2, preparation of resazurin detection reagent: according to the needs of the experiment, take an appropriate amount of resazurin mother solution, add a certain amount of three-distilled water, and prepare a resazurin solution with a final concentration of 0.2%, which is the resazurin detection Reagent;
[0040] Step 3: Determination of PBMC activity under in vitro 3D culture conditions: according to the ratio of the volume of the 3D collagen hydrogel medium...
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